Applied Microbiology and Biotechnology

, Volume 102, Issue 6, pp 2607–2620 | Cite as

Production of extracellular heterologous proteins in Streptomyces rimosus, producer of the antibiotic oxytetracycline

  • Andrés Felipe Carrillo Rincón
  • Vasilka Magdevska
  • Luka Kranjc
  • Štefan Fujs
  • Rolf Müller
  • Hrvoje Petković
Biotechnological products and process engineering


Among the Streptomyces species, Streptomyces lividans has often been used for the production of heterologous proteins as it can secrete target proteins directly into the culture medium. Streptomyces rimosus, on the other hand, has for long been used at an industrial scale for oxytetracycline production, and it holds ‘Generally Recognised As Safe’ status. There are a number of properties of S. rimosus that make this industrial strain an attractive candidate as a host for heterologous protein production, including (1) rapid growth rate; (2) growth as short fragments, as for Escherichia coli; (3) high efficiency of transformation by electroporation; and (4) secretion of proteins into the culture medium. In this study, we specifically focused our efforts on an exploration of the use of the Sec secretory pathway to export heterologous proteins in a S. rimosus host. We aimed to develop a genetic tool kit for S. rimosus and to evaluate the extracellular production of target heterologous proteins of this industrial host. This study demonstrates that S. rimosus can produce the industrially important enzyme phytase AppA extracellularly, and analogous to E. coli as a host, application of His-Tag/Ni-affinity chromatography provides a simple and rapid approach to purify active phytase AppA in S. rimosus. We thus demonstrate that S. rimosus can be used as a potential alternative protein expression system.


Streptomyces rimosus Heterologous protein production Gene expression Gene tools Reporter system Phytase 



This study was funded by the Ministry of Higher Education, Science and Technology (Slovenian Research Agency, ARRS, grant no. L4-7117) to HP and postdoctoral fellowship grant no. C3330-17-529038 ‘Raziskovalci-2.0-UL-BF-529038’ by the Ministry of Higher Education, Science and Technology—Republic of Slovenia to L.K., Slovene Human Resources, Development and Scholarship Funds for the award of the PhD fellowship (grant no. 58-T-003) to V.M. and PhD fellowship to A.F.C.R. in the scope of ‘Augusto González Linares’ (University of Cantabria, Spain).


This study was funded by the Ministry of Higher Education, Science and Technology, Slovenian Research Agency (grant no. L4-7117), grant no. C3330-17-529038 ‘Raziskovalci-2.0-UL-BF-529038’ by the Ministry of Higher Education, Science and Technology—Republic of Slovenia, Slovene Human Resources, Development and Scholarship Funds (grant no. 58-T-003) and ‘Augusto González Linares’ program (University of Cantabria, Spain).

Compliance with ethical standards

This article does not contain any studies with human participants or animals performed by any of the authors.

Conflict of interest

A.F.C.R. declares that he has no conflict of interest. V.M. declares that she has no conflict of interest. L.K. declares that he has no conflict of interest. Š.F. is a shareholder of Acies Bio d.o.o.. R.M. declares that he has no conflict of interest, and H.P. declares that he has no conflict of interest.

Supplementary material

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Instituto de Biomedicina y Biotecnología de Cantabria (IBBTEC)Universidad de Cantabria, CSICSantanderSpain
  2. 2.Department of Microbial Natural Products, Helmholtz-Institute for Pharmaceutical Research Saarland (HIPS), Helmholtz Centre for Infection Research (HZI) and Pharmaceutical BiotechnologySaarland UniversitySaarbrückenGermany
  3. 3.Biotechnical FacultyUniversity of LjubljanaLjubljanaSlovenia
  4. 4.Acies BioLjubljanaSlovenia

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