Applied Microbiology and Biotechnology

, Volume 102, Issue 5, pp 2143–2154 | Cite as

Immunological features and efficacy of the recombinant subunit vaccine LTB-EMY162 against Echinococcus multilocularis metacestode

Biotechnological products and process engineering
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Abstract

Alveolar echinococcosis is a zoonotic disease caused by the infection of the larval stage Echinococcus multilocularis with worldwide distribution especially in the northwest China. It is important to develop a well-tolerated immunoprophylaxis against E. multilocularis for alveolar echinococcosis control. In this study, a prokaryotic expression system for recombinant immunogen LTB-EMY162 was established, and the immunological features, sensitized lymphocyte, IL-4/IFN-γ secreted, prophylactic effect, and therapeutic effect were also evaluated. Arctic Express (DE3) system, Ni2+-charged and molecular sieve chromatography were used to obtain a high-purity 29 kDa protein. The ELISA and lymphocyte proliferation assay showed that LTB-EMY162 induced high-titer specific IgG against EMY162 and E. multilocularis protoscoleces protein in BALB/c mice and promoted sensitized T lymphocyte cell proliferation, and LTB-EMY162 stimulated Th cell to secrete IL-4 and IFN-γ and induced a Th1/Th2 mixed type immunological response. We also found that LTB-EMY162 significantly inhibited the cysts formation by challenging with 1000 E. multilocularis protoscoleces. The growth of protoscoleces and cysts were also significantly decreased by treating with LTB-EMY162 in 1000 protoscoleces intraperitoneal injection therapeutic mice model. In conclusion, we have constructed a subunit vaccine LTB-EMY162 which has prevention and therapeutic effect against E. multilocularis infection.

Keywords

Echinococcus multilocularis Alveolar echinococcosis Subunit vaccine EMY162 

Notes

Acknowledgments

We thank all of the staffs for their helpful suggestions and advice during this project. We would like to thank the editor for the helpful comments on the manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interests.

Ethical approval

All experiments were conducted with adherence to the NIH Guide for the Care and Use of Laboratory Animals, and the protocol was approved by the Animal Ethical and Experimental Committee of Qinghai University (QHDX-2014-07). This article does not contain any studies with human participants performed by any of the authors.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Research Center for High Altitude MedicineQinghai UniversityXiningChina
  2. 2.Qinghai-Utah Joint Research Key Lab for High Altitude MedicineXiningChina
  3. 3.Qinghai Key Laboratory of Science and Technology for High Altitude MedicineXiningChina
  4. 4.Qinghai Key Laboratory for EchinococcosisXiningChina
  5. 5.Ningxia Key Laboratory of Clinical and Pathogenic MicrobiologyGeneral Hospital of Ningxia Medical UniversityYinchuanChina
  6. 6.Ningxia Key Laboratory of Cerebrocranial Diseases, School of Laboratory MedicineNingxia Medical UniversityYinchuanChina
  7. 7.Research Center for High Altitude ZoonosisQinghai UniversityXiningChina

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