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Applied Microbiology and Biotechnology

, Volume 102, Issue 5, pp 2235–2249 | Cite as

Toxoplasma gondii antigen SAG2A differentially modulates IL-1β expression in resistant and susceptible murine peritoneal cells

  • Jamilly Azevedo Leal-Sena
  • Jane Lima dos Santos
  • Thaise Anne Rocha dos Santos
  • Edson Mário de Andrade
  • Tiago Antônio de Oliveira Mendes
  • Juliano Oliveira Santana
  • Tiago Wilson Patriarca Mineo
  • José Roberto Mineo
  • Jair Pereira da Cunha-Júnior
  • Carlos Priminho Pirovani
Genomics, transcriptomics, proteomics

Abstract

The cell surface of Toxoplasma gondii is covered by antigens (SAGs) from the SRS family anchored by glycosylphosphatidylinositol (GPI) and includes antigens from the SAG2 family. Among these, the SAG2A surface antigen shows great potential in activating humoral responses and has been used in characterizing the acute phase of infection and in the serological diagnosis of toxoplasmosis. In this study, we aimed to evaluate rSAG2A-induced proteins in BALB/c and C57BL/c mice macrophages and to evaluate the phenotypic polarization induced in the process. We treated the peritoneal macrophages from mouse strains that were resistant or susceptible to T. gondii with rSAG2A to analyze their proteomic profile by mass spectrometry and systems biology. We also examined the gene expression of these cells by RT-qPCR using the phenotypic markers of M1 and M2 macrophages. Differences were observed in the expression of proteins involved in the inflammatory process in both resistant and susceptible cells, and macrophages were preferentially induced to obtain a pro-inflammatory immune response (M1) via the overexpression of IL-1β in mice susceptible to this parasite. These data suggest that the SAG2A antigen induces phenotypic and classical activation of macrophages in both resistant and susceptible strains of mice during the acute phase of the disease.

Keywords

Toxoplasmosis SAG2A Macrophage polarization Pro-inflammatory immune response 

Notes

Author contributions

Conceived and designed the experiments: JLS, JALS, and CPP. Performed the experiments: JALS, JLS, and TARS. Analyzed the data: JALS, JLS, TAOM, CPP, TWPM, JRM, and JPCJ. Contributed reagents/material/analysis tools: CPP, JLS, EMA, and TM. Wrote the paper: JALS, JLS, and CPP. All the authors read and approved the final version of the manuscript.

Compliance with ethical standards

The animals were bred and obtained from the Central Biotherium of the Federal University of Minas Gerais (UFMG), Belo Horizonte, Brazil, and all the animals were maintained under standard conditions according to the institutional guide to animal ethics approved by the Animal Ethics Committee of the State University of Santa Cruz (protocol number 04/2011).

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

253_2018_8759_MOESM1_ESM.pdf (789 kb)
ESM 1 (PDF 788 kb)
253_2018_8759_MOESM2_ESM.xlsx (62 kb)
ESM 2 (XLSX 62 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Jamilly Azevedo Leal-Sena
    • 1
  • Jane Lima dos Santos
    • 1
  • Thaise Anne Rocha dos Santos
    • 1
  • Edson Mário de Andrade
    • 1
  • Tiago Antônio de Oliveira Mendes
    • 2
  • Juliano Oliveira Santana
    • 1
  • Tiago Wilson Patriarca Mineo
    • 3
  • José Roberto Mineo
    • 3
  • Jair Pereira da Cunha-Júnior
    • 3
  • Carlos Priminho Pirovani
    • 1
  1. 1.Biothecnology and Genetic CenterState University of Santa CruzIlhéusBrazil
  2. 2.Federal University of ViçosaViçosaBrazil
  3. 3.Federal University of UberlândiaUberlândiaBrazil

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