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Applied Microbiology and Biotechnology

, Volume 102, Issue 5, pp 2279–2288 | Cite as

Cold atmospheric pressure plasma causes protein denaturation and endoplasmic reticulum stress in Saccharomyces cerevisiae

  • Koki Itooka
  • Kazuo Takahashi
  • Yukio Kimata
  • Shingo Izawa
Applied microbial and cell physiology

Abstract

Cold atmospheric pressure plasma (CAP) does not cause thermal damage or generate toxic residues; hence, it is projected as an alternative agent for sterilization in food and pharmaceutical industries. The fungicidal effects of CAP have not yet been investigated as extensively as its bactericidal effects. We herein examined the effects of CAP on yeast proteins using a new CAP system with an improved processing capacity. We demonstrated that protein ubiquitination and the formation of protein aggregates were induced in the cytoplasm of yeast cells by the CAP treatment. GFP-tagged Tsa1 and Ssa1, an H2O2-responsive molecular chaperone and constitutively expressed Hsp70, respectively, formed cytoplasmic foci in CAP-treated cells. Furthermore, Tsa1 was essential for the formation of Ssa1-GFP foci. These results indicate that the denaturation of yeast proteins was caused by CAP, at least partially, in a H2O2-dependent manner. Furthermore, misfolded protein levels in the endoplasmic reticulum (ER) and the oligomerization of Ire1, a key sensor of ER stress, were enhanced by the treatment with CAP, indicating that CAP causes ER stress in yeast cells as a specific phenomenon to eukaryotic cells. The pretreatment of yeast cells at 37 °C significantly alleviated cell death caused by CAP. Our results strongly suggest that the induction of protein denaturation is a primary mechanism of the fungicidal effects of CAP.

Keywords

Cold atmospheric pressure plasma Fungicidal efficacy Sterilization Endoplasmic reticulum stress Protein denaturation Saccharomyces cerevisiae 

Notes

Acknowledgments

We are grateful to N. Kawazoe for her constructive advice.

Funding information

This study was supported by the Japan Society for the Promotion of Science [grant number 26292039] and the Kyoto Lifetech Innovation Support Center.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Laboratory of Microbial Technology, Graduate School of Science and TechnologyKyoto Institute of TechnologyKyotoJapan
  2. 2.Faculty of Electrical Engineering and ElectronicsKyoto Institute of TechnologyKyotoJapan
  3. 3.Graduate School of Biological SciencesNara Institute of Science and TechnologyNaraJapan

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