Current state and perspectives in hydrogen production by Escherichia coli: roles of hydrogenases in glucose or glycerol metabolism
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Escherichia coli has been a robust host strain for much biological research, in particular, research in metabolic engineering, protein engineering, and heterologous gene expression. In this mini review, to understand bacterial hydrogen production by E. coli, the effect of glucose and glycerol metabolism on hydrogen production is compared, and the current approaches to enhance hydrogen production from glycerol as a substrate are reviewed. In addition, the argument from past to present on the functions of E. coli hydrogenases, hydrogenase 1, hydrogenase 2, hydrogenase 3, and hydrogenase 4 is summarized. Furthermore, based on the literature that the E. coli formate-hydrogen lyase is essential for bacterial hydrogen production via recombinant hydrogenases, research achievements from the past regarding heterologous production of hydrogenase are rethought.
KeywordsHydrogen Glycerol metabolism Glucose metabolism Heterologous expression Escherichia coli
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Conflict of interest
The authors declare that there are no conflicts of interest.
This article does not contain any studies performed with human participants or with animals by any of the authors.
- Bagramyan K, Trchounian A (2003) Structural and functional features of formate hydrogen lyase, an enzyme of mixed-acid fermentation from Escherichia coli. Biochemistry (Mosc) 68(11):1159–1170. https://doi.org/10.1023/B:BIRY.0000009129.18714.a4 CrossRefGoogle Scholar
- Blattner FR, Plunkett G 3rd, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y (1997) The complete genome sequence of Escherichia coli K-12. Science 277(5331):1453–1474. https://doi.org/10.1126/science.277.5331.1453 CrossRefPubMedGoogle Scholar
- Ghosh D, Bisaillon A, Hallenbeck PC (2013) Increasing the metabolic capacity of Escherichia coli for hydrogen production through heterologous expression of the Ralstonia eutropha SH operon. Biotechnol Biofuels 6(1):122. https://doi.org/10.1186/1754-6834-6-122 CrossRefPubMedPubMedCentralGoogle Scholar
- Holtman CK, Pawlyk AC, Meadow ND, Pettigrew DW (2001) Reverse genetics of Escherichia coli glycerol kinase allosteric regulation and glucose control of glycerol utilization in vivo. J Bacteriol 183(11):3336–3344. https://doi.org/10.1128/JB.183.11.3336-3344.2001 CrossRefPubMedPubMedCentralGoogle Scholar
- Kitagawa M, Ara T, Arifuzzaman M, Ioka-Nakamichi T, Inamoto E, Toyonaga H, Mori H (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res 12(5):291–299. https://doi.org/10.1093/dnares/dsi012 CrossRefPubMedGoogle Scholar
- Lee SY, Lee HJ, Park JM, Lee JH, Park JS, Shin HS, Kim YH, Min J (2010) Bacterial hydrogen production in recombinant Escherichia coli harboring a HupSL hydrogenase isolated from Rhodobacter sphaeroides under anaerobic dark culture. Int J Hydrogen Energy 35(3):1112–1116. https://doi.org/10.1016/j.ijhydene.2009.11.068 CrossRefGoogle Scholar
- Menon NK, Chatelus CY, Dervartanian M, Wendt JC, Shanmugam KT, Peck HD Jr, Przybyla AE (1994) Cloning, sequencing, and mutational analysis of the hyb operon encoding Escherichia coli hydrogenase 2. J Bacteriol 176(14):4416–4423. https://doi.org/10.1128/jb.176.14.4416-4423.1994 CrossRefPubMedPubMedCentralGoogle Scholar
- Mishra J, Khurana S, Kumar N, Ghosh AK, Das D (2004) Molecular cloning, characterization, and overexpression of a novel [Fe]-hydrogenase isolated from a high rate of hydrogen producing Enterobacter cloacae IIT-BT 08. Biochem Biophys Res Commun 324(2):679–685. https://doi.org/10.1016/j.bbrc.2004.09.108 CrossRefPubMedGoogle Scholar
- Mohd Yasin NH, Fukuzaki M, Maeda T, Miyazaki T, Che Maail CMH, Ariffin H, Wood TK (2013) Biohydrogen production from oil palm frond juice and sewage sludge by a metabolically-engineered Escherichia coli strain. Int J Hydrog Energy 38(25):10277–10283. https://doi.org/10.1016/j.ijhydene.2013.06.065 CrossRefGoogle Scholar
- Rossmann R, Sawers G, Böck A (1991) Mechanism of regulation of the formate-hydrogenlyase pathway by oxygen, nitrate, and pH: definition of the formate regulon. Mol Microbiol 5(11):2807–2814. https://doi.org/10.1111/j.1365-2958.1991.tb01989.x CrossRefPubMedGoogle Scholar
- Seol E, Sekar BS, Raj SM, Park S (2016) Co-production of hydrogen and ethanol from glucose by modification of glycolytic pathways in Escherichia coli—from Embden-Meyerhof-Parnas pathway to pentose phosphate pathway. Biotechnol J 11(2):249–256. https://doi.org/10.1002/biot.201400829 CrossRefPubMedGoogle Scholar
- Suppmann B, Sawers G (1994) Isolation and characterization of hypophosphite-resistant mutants of Escherichia coli: identification of the FocA protein, encoded by the pfl operon, as a putative formate transporter. Mol Microbiol 11(5):965–982. https://doi.org/10.1111/j.1365-2958.1994.tb00375.x CrossRefPubMedGoogle Scholar
- Trchounian K, Trchounian A (2009) Hydrogenase 2 is most and hydrogenase 1 is less responsible for H2 production by Escherichia coli under glycerol fermentation at neutral and slightly alkaline pH. Int J Hydrog Energy 34(21):8839–8845. https://doi.org/10.1016/j.ijhydene.2009.08.056 CrossRefGoogle Scholar
- Wells MA, Mercer J, Mott RA, Pereira-Medrano AG, Burja AM, Radianingtyas H, Wright PC (2011) Engineering a non-native hydrogen production pathway into Escherichia coli via a cyanobacterial [NiFe] hydrogenase. Metab Eng. https://doi.org/10.1016/j.ymben.2011.01.004