Applied Microbiology and Biotechnology

, Volume 102, Issue 5, pp 2041–2050 | Cite as

Current state and perspectives in hydrogen production by Escherichia coli: roles of hydrogenases in glucose or glycerol metabolism

  • Toshinari Maeda
  • Kien Trung Tran
  • Ryota Yamasaki
  • Thomas K. Wood


Escherichia coli has been a robust host strain for much biological research, in particular, research in metabolic engineering, protein engineering, and heterologous gene expression. In this mini review, to understand bacterial hydrogen production by E. coli, the effect of glucose and glycerol metabolism on hydrogen production is compared, and the current approaches to enhance hydrogen production from glycerol as a substrate are reviewed. In addition, the argument from past to present on the functions of E. coli hydrogenases, hydrogenase 1, hydrogenase 2, hydrogenase 3, and hydrogenase 4 is summarized. Furthermore, based on the literature that the E. coli formate-hydrogen lyase is essential for bacterial hydrogen production via recombinant hydrogenases, research achievements from the past regarding heterologous production of hydrogenase are rethought.


Hydrogen Glycerol metabolism Glucose metabolism Heterologous expression Escherichia coli 


Funding information

This study was funded by the Japan Society for the Promotion of Science (16K12650).

Compliance with ethical standards

Conflict of interest

The authors declare that there are no conflicts of interest.

Ethical approval

This article does not contain any studies performed with human participants or with animals by any of the authors.


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© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Biological Functions Engineering, Graduate School of Life Science and Systems EngineeringKyushu Institute of TechnologyKitakyushuJapan
  2. 2.Vinmec Research Institute of Stem Cell and Gene TechnologyHanoiVietnam
  3. 3.Department of Chemical EngineeringPennsylvania State UniversityUniversity ParkUSA

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