, Volume 70, Issue 4, pp 271–277 | Cite as

Identification of the major histocompatibility complex class-II DM and DO alleles in a cohort of northern pig-tailed macaques (Macaca leonina)

  • Xiao-Dong Lian
  • Xi-He Zhang
  • Zheng-Xi Dai
  • Yong-Tang Zheng
Short Communication


The northern pig-tailed macaque (Macaca leonina) has been considered as an independent species from the pig-tailed macaque group. We have previously reported that this species macaque has the potential to be a useful animal model in HIV/AIDS pathogenesis and vaccine studies due to its susceptibility to HIV-1. To develop this animal into a potential HIV/AIDS model, we have studied the classical MHC genes of this animal. In this study, the non-classical MHC genes Malo-DM and Malo-DO alleles were first characterized by sequencing and cloning in 12 unrelated northern pig-tailed macaques. A total of 20 full-length sequences identified include 4 Malo-DMA, 5 Malo-DMB, 7 Malo-DOA, and 4 Malo-DOB alleles. Most of these allele sequences were shared between northern pig-tailed macaque and other macaque species in exon 2. The full-length MHC-DM and MHC-DO sequences provide more comprehensive analysis of immunogenetics of northern pig-tailed macaques and increase the value of the macaques in further biomedical studies.


Northern pig-tailed macaque Macaca leonina MHC-DM MHC-DO 



The authors thank Natasja de Groot, NelOtting, and IMGT Non-Human Primate Nomenclature Committee for naming the Malo-DM and Malo-DO alleles.

Author contributions

Xiao-Dong Lian performed the experiments and wrote the paper; Xiao-Dong Lian, Xi-He Zhang, and Zheng-Xi Dai analyzed the data; and Yong-Tang Zheng conceived and designed the experiments.

Funding information

This work was supported by the Key Scientific and Technological Program of China (2017ZX10304402-002), the National Basic Research Program of China (2012CBA01305), the National Natural Science Foundation of China (81373124, 81671627, 81172876, 81471620, 81571606), the Knowledge Innovation Program of CAS (ZDRW-ZS-2016-4-2), and the Natural Science Foundation of Yunnan Province (2014FB181, P0120150150).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  • Xiao-Dong Lian
    • 1
    • 2
  • Xi-He Zhang
    • 1
    • 2
  • Zheng-Xi Dai
    • 1
  • Yong-Tang Zheng
    • 1
    • 3
  1. 1.Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences/Key Laboratory of Bioactive Peptides of Yunnan Province, Kunming Institute of ZoologyChinese Academy of SciencesKunmingChina
  2. 2.University of Chinese Academy of SciencesBeijingChina
  3. 3.Kunming Primate Research Center, Kunming Institute of ZoologyChinese Academy of SciencesKunmingChina

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