β-Lactoglobulin associative interactions: a small-angle scattering study
- 112 Downloads
β-Lactoglobulin is one of the most notable representatives of the lipocalin family of proteins that are mainly involved in the transportation of small hydrophobic molecules. Therefore, the conformation stability of these proteins represents an important step for several industrial applications. In this study, the β-lactoglobulin (β-lg) conformation stability in solution was studied as a function of the protein concentration, pH, NaCl and temperature when the protein concentration is significantly higher in the buffer solution. Using small-angle X-ray and neutron scattering methods (SANS and SAXS), it was shown that β-lg forms dimeric structures in 50 mM HEPES buffer under various conditions of the medium. At pH 5.9, β-lg preserves its dimeric form in the high protein concentration range of 10–40 mg/mL. However, the conformation swelled with the addition of 50 mM NaCl in the system with 20 mg/mL β-lg concentration. Further increase of NaCl concentration leads to a decrease of the radius of gyration of the β-lg dimer. At pH below 5.9 where the protein is positively charged, β-lg dimer preserves its conformation, and only an increase of the radius of gyration was attested by SAXS as the acidity of the medium increased to pH 3.6. This supports and adds to the findings presented above regarding the stability of β-lg dimer in 50 mM HEPES buffer in D2O. Furthermore, at neutral and alkaline pH, the values of both radius of gyration and maximum particle dimension decrease by increasing the pH of the medium.
Keywordsβ-Lactoglobulin dimer Conformation in solution Small-angle scattering SAXS SANS
This work was supported by a grant framework of the Romanian Plenipotentiary at JINR-Dubna, within the JINR Theme no. 04-4-1121-2015/2020. The authors gratefully acknowledge the beamtime received at IBR-2 pulsed reactor, FLNP, JINR, Dubna, Russian Federation, beamline YuMO small angle neutron scattering spectrometer for the proposals nos. 2015-10-15-23-59-22 and 2016-10-15-20-20-30 and to O. Ivankov, V. Skoj and M. Rulev for providing assistance during the measurements. The experiments were performed on beamline BM29 at the European Synchrotron Radiation Facility (ESRF), Grenoble, France. The authors are grateful to Structural biology Group and Local Contact at the ESRF for providing assistance in using the beamline.
- Báez GD, Moro A, Ballerini GA, Busti PA, Delorenzi NJ (2011) Comparison between structural changes of heat-treated and transglutaminase cross-linked beta-lactoglobulin and their effects on foaming properties. Food Hydrocolloid 25(7):1758–1765. https://doi.org/10.1016/j.foodhyd.2011.02.033 CrossRefGoogle Scholar
- Barbiroli A, Beringhelli T, Bonomi F, Donghi D, Ferranti P, Galliano M, Iametti S, Maggioni D, Rasmussen P, Scanu S, Vilardo MC (2009) Bovine β-lactoglobulin acts as an acid-resistant drug carrier by exploiting its diverse binding regions. Biol Chem 391(1):21–32. https://doi.org/10.1515/bc.2010.008 Google Scholar
- Moitzi Ch, Donato L, Schmitt Ch, Bovetto L, Gillies G, Stradner A (2011) Structure of β-lactoglobulin microgels formed during heating as revealed by small-angle X-ray scattering and light scattering. Food Hydrocolloid 25(7):1766–1774. https://doi.org/10.1016/j.foodhyd.2011.03.020 CrossRefGoogle Scholar
- Pérez OE, David-Birman T, Kesselman E, Levi-Tal S, Lesmes U (2014) Milk protein-vitamin interactions: formation of beta-lactoglobulin/folic acid nano-complexes and their impact on in vitro gastro-duodenal proteolysis. Food Hydrocolloid 38:40–47. https://doi.org/10.1016/j.foodhyd.2013.11.010 CrossRefGoogle Scholar
- Petoukhov MV, Franke D, Shkumatov AV, Tria G, Kikhney AG, Gajda M, Gorba C, Mertens HDT, Konarev PV, Svergun DI (2012) New developments in the ATSAS program package for small-angle scattering data analysis. J Appl Crystallogr 45:342–350. https://doi.org/10.1107/S0021889812007662 CrossRefGoogle Scholar
- van den Akker CC, Schleeger M, Bonn M, Koenderink GH (2014) Structural basis for the polymorphism of β-lactoglobulin amyloid-like fibrils. In: Uversky VN, Lyubchenko YL (eds) Bio-nanoimaging. Academic Press, Boston, pp 333–343. https://doi.org/10.1016/B978-0-12-394431-3.00031-6 CrossRefGoogle Scholar
- Zabelskii DV, Vlasov AV, Ryzhykau YuL, Murugova TN, Brennich M, Soloviov DV, Ivankov OI, Borshchevskiy VI, Mishin AV, Rogachev AV, Round A, Dencher NA, Büldt G, Gordeliy VI, Kuklin AI (2018) Ambiguities and completeness of SAS data analysis: investigations of apoferritin by SAXS/SANS EID and SECSAXS methods. J Phys Conf Ser 994:012017CrossRefGoogle Scholar
- Zhang F, Roosen-Runge F, Skoda MWA, Jacobs MJ, Wolf M, Callow Ph, Frielinghaus H, Pipich V, Prévostf S, Schreibera F (2012) Hydration and interactions in protein solutions containing concentrated electrolytes studied by small-angle scattering. Phys Chem Chem Phys 14(7):2483–2493CrossRefGoogle Scholar