Azospirillum brasilense Increases CO2 Fixation on Microalgae Scenedesmus obliquus, Chlorella vulgaris, and Chlamydomonas reinhardtii Cultured on High CO2 Concentrations
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Mutualism interactions of microalgae with other microorganisms are widely used in several biotechnological processes since symbiotic interaction improves biotechnological capabilities of the microorganisms involved. The interaction of the bacterium Azospirillum brasilense was assessed with three microalgae genus, Scenedesmus, Chlorella, and Chlamydomonas, during CO2 fixation under high CO2 concentrations. The results in this study have demonstrated that A. brasilense maintained a mutualistic interaction with the three microalgae assessed, supported by the metabolic exchange of indole-3-acetic acid (IAA) and tryptophan (Trp), respectively. Besides, CO2 fixation increased, as well as growth and cell compound accumulation, mainly carbohydrates, in each microalgae evaluated, interacting with the bacterium. Overall, these results propose the mutualism interaction of A. brasilense with microalgae for improving biotechnological processes based on microalgae as CO2 capture and their bio-refinery capacity.
KeywordsIndole-3-acetic acid Mutualistic interaction Phytohormones Plant growth-promoting bacterium Tryptophan
The authors are thankful to the Gaseous Biofuels Cluster-CEMIE-BIO for their support under the project SENER-CONACyT 247006. Francisco J. Choix acknowledges CONACyT for the support under the Program-Project 2517 Cátedras CONACYT and Diana Fischer for editorial services in English.
Compliance with Ethical Standards
Conflict of Interest
The authors declare that they have no conflict of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
- 15.Choix FJ, de-Bashan LE, Bashan Y (2012a) Enhanced accumulation of starch and total carbohydrates in alginate-immobilized Chlorella spp. induced by Azospirillum brasilense: II. Autotrophic conditions. Enzym. Microb. Technol. 51:294–299. https://doi.org/10.1016/j.enzmictec.2012.07.012 CrossRefGoogle Scholar
- 16.Choix FJ, de-Bashan LE, Bashan Y (2012b) Enhanced accumulation of starch and total carbohydrates in alginate-immobilized Chlorella spp. induced by Azospirillum brasilense: II. Heterotrophic conditions. Enzym. Microb. Technol. 51:300–309. https://doi.org/10.1016/j.enzmictec.2012.07.012 CrossRefGoogle Scholar
- 18.de-Bashan LE, Bashan Y, Moreno M, Lebsky VK, Bustillos JJ (2002) Increased pigment and lipid content, lipid variety, and cell and population size of the microalgae Chlorella spp. when co-immobilized in alginate beads with the microalgae-growth-promoting bacterium Azospirillum brasilense. Can. J. Microbiol. 48:514–521. https://doi.org/10.1139/W02-051 CrossRefPubMedGoogle Scholar
- 20.de- Bashan LE, Magallon P, Antoun H, Bashan Y (2008b) Role of glutamate dehydrogenase and glutamine synthetase in Chlorella vulgaris during assimilation of ammonium when jointly immobilized with the microalgae-growth-promoting bacterium Azospirillum brasilense. J. Phycol. 44:1188–1196. https://doi.org/10.1111/j.1529-8817.2008.00572.x CrossRefPubMedGoogle Scholar
- 21.de -Bashan LE, Mayali X, Bebout BM, Weber PK, Detweiler AM, Hernandez J-P, Prufert-Bebout L, Bashan Y (2016) Establishment of stable synthetic mutualism without co-evolution between microalgae and bacteria demonstrated by mutual transfer of metabolites (NanoSIMS isotopic imaging) and persistent physical association (fluorescent in situ hybridization). Algal Res. 15:179–186. https://doi.org/10.1016/j.algal.2016.02.019 CrossRefGoogle Scholar
- 22.Palacios OA, Gomez-Anduro G, Bashan Y, de -Bashan LE (2016b) Tryptophan, thiamine and indole-3-acetic acid exchange between Chlorella sorokiniana and the plant growth-promoting bacterium Azospirillum brasilense. FEMS Microbiol. Ecol. 92:1–11. https://doi.org/10.1093/femsec/fiw077 CrossRefGoogle Scholar
- 24.Palacios OA, Choix FJ, Bashan Y, de -Bashan LE (2016a) Influence of tryptophan and indole-3-acetic acid on starch accumulation in the synthetic mutualistic Chlorella sorokiniana-Azospirillum brasilense system under heterotrophic conditions. Res. Microbiol. 167:367–379. https://doi.org/10.1016/j.resmic.2016.02.005 CrossRefPubMedGoogle Scholar
- 27.Choix FJ, Polster E, Corona-González RI, Snell-Castor R, Méndez-Acosta HO (2017) Nutrient composition of culture media induces different patterns of CO2 fixation from biogas and biomass production by the microalga Scenedesmus obliquus U169. Bioprocess Biosyst. Eng. 40:1733–1742. https://doi.org/10.1007/s00449-017-1828-5 CrossRefPubMedGoogle Scholar
- 28.Tang D, Han W, Li P, Miao X, Zhong J (2011) Bioresource technology CO2 biofixation and fatty acid composition of Scenedesmus obliquus and Chlorella pyrenoidosa in response to different CO2 levels. Bioresour. Technol. 102:3071–3076. https://doi.org/10.1016/j.biortech.2010.10.047 CrossRefPubMedGoogle Scholar
- 32.Bligh EG, Dyer WJ (1959) A rapid method of total lipid extraction and purification. Can J Biochem Cell Phys 37:911–917Google Scholar
- 38.Omar MNA, Osman MEH, Kasim WA, Abd El-Daim IA (2009) Improvement of salt tolerance mechanisms of barley cultivated under salt stress using Azospirillum brasilense. In: Ashraf M et al (eds) Salinity and water stress. Springer, Netherlands, pp 111–116Google Scholar
- 39.Rozier C, Erban E, Hamzaoui J, Prignet-Combartet C, Comte G, Kopka J, Czarnes S, Legendre L (2016) Xylem sap metabolite profile changes during photostimulation of maize by the plant growth-promoting Rhyzobacterium, Azospirillum lipoferum. Metabolomics (Los Angel) 182. https://doi.org/10.4172/2153-0769.1000182
- 41.Broek VA, Gysegom P, Ona O, Hendrickx N, Prinsen E, Impe JV, Vanderleyden J (2005) Transcriptional analysis of the Azospirillum brasilense indole-3-pyruvate decarboxylase gene and Identification of a cis-acting sequence involved in auxin responsive expression. Mol. Plant-Microbe Interact. 18:311–323. https://doi.org/10.1094/MPMI-18-0311 CrossRefGoogle Scholar