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The correlation between DTI parameters and levels of AQP-4 in the early phases of cerebral edema after hypoxic-ischemic/reperfusion injury in piglets

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Abstract

Background

Brain edema during the early stages of hypoxic-ischemic/reperfusion (HI/R) injury can be determined using diffusion tensor imaging (DTI). The change in ADC values has been correlated with the change in expression of AQP-4.

Objective

To determine cerebral edema at specific time intervals after HI/R injury using DTI modalities and discuss its relationship to the expression of aquaporin-4 (AQP-4).

Materials and methods

Thirty newborn piglets were divided into six groups (2, 6, 12, 24, 48 and 72 h) after HI/R injury. The control group subjected to sham surgery included five piglets. DTI scans and immunohistochemistry of AQP-4 expression were performed on piglet brain. The relationship between DTI parameters (FA and ADC values) and the optical density (OD) of AQP-4 expression was determined.

Results

In the striatum, ADC values dropped and reached their lowest level at 24 h (F = 27.42, P < 0.05). In the subcortical border region, ADC values increased after a transient decrease and peaked at 48 h, demonstrating a significant difference from the control group (F = 50.25, P < 0.05). FA values in the internal capsules and subcortical white matter in HI/R models decreased continuously after HI/R, although no statistically significant difference from the control group was achieved. ADC and OD values of AQP-4 expression were positively correlated (r = 0.875, P < 0.05).

Conclusions

The change in ADC value after HI/R injury correlates with the expression of AQP-4.

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Acknowledgments

This work was supported by the State Natural Sciences Foundation of China (No. 30570541). We appreciate the valuable comments from other members of our laboratories.

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Authors and Affiliations

  1. Department of Radiology, Shengjing Hospital of China Medical University, Shenyang, 110004, Liaoning Province, China

    Hongwei Wang, Xiaoming Wang & Qiyong Guo

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  1. Hongwei Wang
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  2. Xiaoming Wang
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  3. Qiyong Guo
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Correspondence to Xiaoming Wang.

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Wang, H., Wang, X. & Guo, Q. The correlation between DTI parameters and levels of AQP-4 in the early phases of cerebral edema after hypoxic-ischemic/reperfusion injury in piglets. Pediatr Radiol 42, 992–999 (2012). https://doi.org/10.1007/s00247-012-2373-7

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  • DOI: https://doi.org/10.1007/s00247-012-2373-7

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