Skip to main content
SpringerLink
Log in

Bone tissue concentrations of ciprofloxacin released from biodegradable screws implanted in rabbits skull

  • Original Paper
  • Published:
European Journal of Plastic Surgery Aims and scope Submit manuscript

Abstract

Previously, ciprofloxacin-releasing polylactide/polyglycolide 80/20 (SR-PLGA) screws have proven to be biocompatible with sufficient strength. However, there has been no information about the local concentrations of ciprofloxacin in bone tissue after their implantation. To measure bone concentrations of ciprofloxacin, two screws were implanted in each rabbit, one on either side of the sagittal suture (n = 28 rabbits). Follow-up periods were 2, 4, 8, 16, 24, 52 and 78 weeks. From each rabbit, bone blocks containing one screw were retrieved and used to measure drug concentration. Ciprofloxacin concentration at 2 weeks follow-up was 4.4 ± 4.2 μg/g, 14.1 ± 2.7 μg/g at 4 weeks and 7.6 ± 4.5 μg/g at 8 weeks. Measured concentrations were very low at 16 weeks (mean 0.09 ± 0.04 μg/g) and 24 weeks (mean 0.04 ± 0.02 μg/g). Surprisingly, the concentration was higher again at 1 year (mean 1.3 ± 1.0 μg/g). At 1.5 years, concentration had decreased again (mean 0.8 ± 0.6 μg/g). The drug concentration in bone tissue was higher than minimal inhibitory concentration of ciprofloxacin (Staphylococcus aureus 0.1–1.0 μg/g) at 2, 4, and 8 weeks. Ciprofloxacin-releasing SR-PLGA 80/20 screws could be used clinically for osteofixation and infection treatment in cranial bone.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Overbeck J, Winckler S, Meffert R, Törmälä P, Spiegel H, Brug E (1995) Penetration of ciprofloxacin into bone: a new bioabsorbable implant. J Investig Surg 8(3):155–162

    Article  CAS  Google Scholar 

  2. Wei G, Kotoura Y, Oka M, Yamamuro T, Wada R, Hyon S, Ikada Y (1991) A Bioabsorbable delivery system for antibiotic treatment of osteomyelitis. J Bone Jt Surg Br 73B:246–552

    Google Scholar 

  3. Becker P, Smith R, Williams R, Dutkowsky J (1994) Comparison of antibiotic release from polymethylmethacrylate beads and sponge collagen. J Orthop Res 12(5):737–741

    Article  PubMed  CAS  Google Scholar 

  4. Shinto Y, Uchida A, Korkusuz F, Araki N, Ono K (1992) Calcium hydroxyapatite ceramic used as a delivery system for antibiotics. J Bone Jt Surg Br 74(4):600–604

    CAS  Google Scholar 

  5. Jacob E, Setterström J, Bach D, Heath J, McNiesh L, Cierny G (1991) Evaluation of biodegradable ampicillin anhydrate microcapsules for local treatment of experimental staphylococcal osteomyelitis. Clin Orthop Rel Res 267:237–244

    Google Scholar 

  6. Ostermann P, Henry S, Seligson D (1993) The role of local antibiotic therapy in the management of compound fractures. Clin Orthop 295:102–111

    PubMed  Google Scholar 

  7. Nie L, Nicolau D, Tessier P, Kourea H, Browner B, Nightingale C (1998) Use of a bioabsorbable polymer for the delivery of ofloxacin during experimental osteomyelitis treatment. J Orthop Res 16(1):76–79

    Article  PubMed  CAS  Google Scholar 

  8. Calhoun J, Mader J (1997) Treatment of osteomyelitis with a biodegradable antibiotic implant. Clin Orthop 341:206–214

    PubMed  Google Scholar 

  9. Gümüsderelioglu M, Deniz G (2000) Sustained release of mitomycin-C from poly (DL-lactide)/poly (DL-lactide-co-glycolide) films. J Biomater Sci 11(10):1039–1050

    Article  Google Scholar 

  10. Teupe C, Meffert R, Winckler S, Ritzerfeld W, Törmälä P, Brug E (1992) Ciprofloxacin-impregnated poly-l-lactic acid drug carrier. New aspects of a resorbable drug delivery system in local antimicrobial treatment of bone infections. Arch Orthop Trauma Surg 112(1):33–35

    Article  PubMed  CAS  Google Scholar 

  11. Tiainen J, Veiranto M, Suokas E, Törmälä P, Waris T, Ninkovic M, Ashammakhi N (2002) Bioabsorbable ciprofloxacin-containing and plain self-reinforced polylactide–polyglycolide 80/20 screws: pull-out strength properties in human cadaver parietal bones. J Craniofac Surg 13(3):427–433

    Article  PubMed  Google Scholar 

  12. Leinonen S, Suokas E, Veiranto M, Törmälä P, Waris T, Ashammakhi N (2002) Holding power of bioabsorbable ciprofloxacin-containing self-reinforced poly-L/DL-lactide 70/30 bioactive glass 13 miniscrews in human cadaver bone. J Craniofac Surg 13(2):212–218

    Article  PubMed  Google Scholar 

  13. Ashammakhi N (1996) Neomembranes: a concept review with special reference to self-reinforced polyglycolide membranes. J Biomed Mater Res 33(4):297–303

    Article  PubMed  CAS  Google Scholar 

  14. Veiranto M, Suokas E, Törmälä P (2002) In vitro mechanical and drug release properties of bioabsorbable ciprofloxacin containing and neat self-reinforced P(L/DL)LA 70/30 fixation screws. J Mater Sci Mater Med 13(12):1259–1263

    Article  PubMed  CAS  Google Scholar 

  15. Humphrey JS, Mehta S, Seaber AV, Vail TP (1998) Pharmacokinetics of a degradable drug delivery system in bone. Clin Orthop 349:218–224

    Article  PubMed  Google Scholar 

  16. Pietrzak W, Sarver D, Verstynen M (1997) Bioabsorbable polymer science for the practicing surgeon. J Craniofac Surg 8(2):87–91

    Article  PubMed  CAS  Google Scholar 

  17. Ramchandani M, Robinson D (1998) In vitro and in vivo release of ciprofloxacin from PLGA 50:50 implants. J Control Rel 54(2):167–175

    Article  CAS  Google Scholar 

  18. Törmälä P (1992) Biodegradable self-reinforced composite materials; manufacturing structure and mechanical properties. Clin Mater 10(1–2):29–34

    Article  PubMed  Google Scholar 

  19. Kitchell JP, Wise DL (1985) Poly (lactic/glycolic acid) biodegradable drug–polymer matrix systems. Methods Enzymol 112:436–448

    Article  PubMed  CAS  Google Scholar 

  20. Veiranto M, Suokas E, Törmälä P (2002) Controlled release of ciprofloxacin from fixation screws based on poly (lactide-co-glycolide). Society for Biomaterials 28th Annual Meeting, Tampa, Florida, USA, April 24–27, 2002, p 637

  21. Tiainen J, Soini Y, Suokas E, Veiranto M, Törmälä P, Waris T, Ashammakhi N (2004) Tissue reactions to multifunctional bioabsorbable ciprofloxacin-releasing polylactide–polyglycolide 80/20 screws in rabbits’ cranial bone. J Mater Sci Mater Med 17(12):1315–1322

    Article  Google Scholar 

  22. Veiranto M, Suokas E, Ashammakhi N, Törmälä P (2004) Novel bioabsorbable antibiotic releasing bone fracture fixation implants. Adv Exp Med Biol 553:197–208

    PubMed  CAS  Google Scholar 

  23. Tiainen J, Soini Y, Törmälä P, Waris T, Ashammakhi N (2003) Self-reinforced polylactide–polyglycolide 80/20 screws take more than 1  years to resorb in rabbit cranial bone. http://www.tissue-engineering-oc.com/abstracts/abstract_self_reinforced_polylact.html, (accessed on July 5th, 2003)

  24. Tiainen J, Soini Y, Törmälä P, Waris T, Ashammakhi N (2004) Self-reinforced polylactide–polyglycolide 80/20 screws take more than 1 years to resorb in rabbit cranial bone. J Biomed Mater Res 70B(1):49–55

    Article  CAS  Google Scholar 

Download references

Acknowledgments

Research funds from the Research funds from the European Commission (EXPERTISSUES Project NMP3-CT-2004–500283), Technology Development Center in Finland (TEKES, MFM Project 474/31/03) and the Academy of Finland (Center of Excellence 40056/04), and the Ministry of Education (Graduate School of Biomaterials and Tissue Engineering) are greatly appreciated. The authors thank Mrs. Seija Seljanperä, registered nurse, for assistance with surgical operations, Nick Bolton, Ph.D., for checking the language of the manuscript, Ms. Anja Jussila and Ms. Mari Keijola for administrative assistance.

Sources of support: European Commission (EXPERTISSUES Project NMP3-CT-2004–500283), Technology Development Center in Finland (TEKES, MFM Project 474/31/03) and the Academy of Finland (Center of Excellence 40056/04), and the Ministry of Education (Graduate School of Biomaterials and Tissue Engineering).

Author information

Authors and Affiliations

  1. Department of Surgery, Oulu University Hospital, P.O. Box 5000, 90014, Oulu, Finland

    Johanna Tiainen, Outi Kaarela, Timo Waris & Nureddin Ashammakhi

  2. Institute of Biomaterials, Tampere University of Technology, Tampere, Finland

    Minna Veiranto, Pertti Törmälä & Nureddin Ashammakhi

  3. Orthopedic Research Unit, University of Turku, Turku, Finland

    J. K. Koort

  4. Linvatec Biomaterials Ltd., Tampere, Finland

    Esa Suokas

Authors
  1. Johanna Tiainen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Minna Veiranto
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. J. K. Koort
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Esa Suokas
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Outi Kaarela
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Pertti Törmälä
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Timo Waris
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Nureddin Ashammakhi
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Johanna Tiainen.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Tiainen, J., Veiranto, M., Koort, J.K. et al. Bone tissue concentrations of ciprofloxacin released from biodegradable screws implanted in rabbits skull. Eur J Plast Surg 35, 171–175 (2012). https://doi.org/10.1007/s00238-007-0188-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00238-007-0188-9

Keywords

Search

Navigation