Abstract
Amphipols (APols) have become important tools for the stabilization, folding, and in vitro structural and functional studies of membrane proteins (MPs). Direct crystallization of MPs solubilized in APols would be of high importance for structural biology. However, despite considerable efforts, it is still not clear whether MP/APol complexes can form well-ordered crystals suitable for X-ray crystallography. In the present work, we show that an APol-trapped MP can be crystallized in meso. Bacteriorhodopsin (BR) trapped by APol A8-35 was mixed with a lipidic mesophase, and crystallization was induced by adding a precipitant. The crystals diffract beyond 2 Å. The structure of BR was solved to 2 Å and found to be indistinguishable from previous structures obtained after transfer from detergent solutions. We suggest the proposed protocol of in meso crystallization to be generally applicable to APol-trapped MPs.
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Acknowledgments
Particular thanks are due to Fabrice Giusti (UMR 7099) for synthesizing the amphipols used in the present work. The diffraction experiments were performed at the beamline ID23-1 of the European Synchrotron Radiation Facility (ESRF), Grenoble, France. We are grateful to the ESRF beamline staff for assistance. This work was supported by the program “Chaires d’excellence, édition 2008’’ of the Agence Nationale de la Recherche France, by the Commissariat à l’Énergie Atomique (Institut de Biologie Structurale), by the Helmholtz Gemeinschaft (Research Centre Jülich) Special Topic of Cooperation 5.1 specific agreement, by a Marie Curie grant (Seventh Framework Programme-PEOPLE-2007-1-1-Initial Training Networks, project Structural Biology of Membrane Proteins), by a European Commission Seventh Framework Programme grant for the European Drug Initiative on Channels and Transporters consortium (HEALTH-201924), by the Centre National pour la Recherche Scientifique, by University Paris–7, and by the “Initiative d’Excellence” program of the French State (Grant “DYNAMO”, ANR-11-LABX-0011-01). Vitaly Polovinkin is deeply thankful to the Fondation Nanosciences for financial support. Part of this work was supported by the German Ministry of Education and Research (PhoNa-Photonic Nanomaterials). Protein expression, crystallization experiments and data treatment were supported by Russian Scientific Foundation (project 14-14-00995). We acknowledge support of this work by the Russian Foundation for Basic Research (Research project 13-04-01700), by the Russian program “5Top100” and by the Ministry of Education and Science of the Russian Federation. This work was supported by ONEXIM, Russia.
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V. Polovinkin and I. Gushchin have contributed equally to this work.
An erratum to this article is available at http://dx.doi.org/10.1007/s00232-017-9949-y.
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Polovinkin, V., Gushchin, I., Sintsov, M. et al. High-Resolution Structure of a Membrane Protein Transferred from Amphipol to a Lipidic Mesophase. J Membrane Biol 247, 997–1004 (2014). https://doi.org/10.1007/s00232-014-9700-x
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DOI: https://doi.org/10.1007/s00232-014-9700-x