Abstract
On-line determination of respiration rates at steady-state dissolved oxygen tensions was performed in a thermostatted measuring chamber equipped with a galvanic oxygen electrode. The measuring chamber is designed to completely exclude gas bubbles from the liquid phase and is named a mono-phase oxystat. Additions of oxygenated water to maintain steady-state dissolved oxygen tensions are controlled by a computer, and the respiration rate is determined from the rate of these additions. An external loop with a flow cell in a spectrophotometer makes it possible to determine and control the concentration of algal cells in the mono-phase oxystat and allowed simultaneous determination of respiration and filtration rates of filter-feeding invertebrates. Because the system maintains steady-state conditions the experiments do not suffer from time limitations. The response time of the system is negligible compared to the response time of other open systems.
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Received: 7 November 1996 / Accepted: 6 December 1996
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Eriksen, N., Iversen, J. On-line determination of respiration rates of aquatic organisms in a mono-phase oxystat at steady-state dissolved oxygen tensions. Marine Biology 128, 181–189 (1997). https://doi.org/10.1007/s002270050081
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DOI: https://doi.org/10.1007/s002270050081