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The regulatory role of arginine kinase during larval settlement of the bryozoan Bugula neritina

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Abstract

Larval attachment and metamorphosis, also collectively known as settlement, plays an important role in the life history of marine invertebrates with biphasic life cycle. Bugula neritina can settle on any substrate without substrate selection. This complex transformation usually involves an active selection process, while the larval decision to settle is regulated by both exogenous and endogenous factors. This study focuses on the involvement of arginine kinase (AK), an important enzyme that regulates energy metabolism in marine invertebrates, during larval settlement of the bryozoan Bugula neritina. We reveal that AK was highly expressed in the swimming larvae of B. neritina, while its expression was down-regulated post-attachment. When treated with AK inhibitors, the larval settlement rate was significantly decreased. In addition, the immunostaining results indicated that AK was mainly localized to the neuro-muscular cord in swimming larvae. Overall, our results suggest the involvement of AK in regulating larval settlement of B. neritina.

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Acknowledgements

This work was supported by Research Grants Council of the Hong Kong Special Administrative Region (Grant nos. GRF16101015 and GRF662413) to Pei-Yuan Qian and as well as grants from Scientific and Technical Innovation Council of Shenzhen (Grant nos. 827000012, JCYJ20150625102622556, KQJSCX2017033011020642) and Guangdong Natural Science Foundation (Grant no. 2014A030310230) to Yu Zhang.

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  1. Yu Zhang and Xiao-Xue Yang have contributed equally to this work.

Authors and Affiliations

  1. Shenzhen Key Laboratory of Marine Bioresource and Eco-environmental Science, Guangdong Engineering Research Center for Marine Algal Biotechnology, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, People’s Republic of China

    Yu Zhang

  2. Department of Ocean Science and Division of Life Science, The Hong Kong University of Science and Technology, Hong Kong SAR, People’s Republic of China

    Yu Zhang, Xiao-Xue Yang, Yue-Him Wong & Pei-Yuan Qian

  3. Department of Biotechnology, Akita Prefectural University, Akita, Japan

    Yue-Him Wong

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  1. Yu Zhang
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Correspondence to Pei-Yuan Qian.

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Zhang, Y., Yang, XX., Wong, YH. et al. The regulatory role of arginine kinase during larval settlement of the bryozoan Bugula neritina. Mar Biol 165, 52 (2018). https://doi.org/10.1007/s00227-018-3307-1

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