Photosynthetic activity and photoprotection in green and red leaves of the seagrasses, Halophila ovalis and Cymodocea rotundata: implications for the photoprotective role of anthocyanin
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Although anthocyanin accumulation is common among intertidal seagrasses in the tropical bioregions, its physiological role remains to be elucidated. While several works suggested that leaf anthocyanin plays a photoprotective role, others concluded that it compensates for lower capacity of other photoprotective mechanisms. To test the photoprotection hypothesis, we assessed the physiological responses of the seagrasses, Halophila ovalis and Cymodocea rotundata, which exhibit green (anthocyanin poor) and red (anthocyanin rich) plants in the same meadow. Diurnal variations in maximum quantum yield showed similar level of photoinhibition between the green and red leaves. Greater effective quantum yield and chlorophyll b/a ratio detected in the red leaves of H. ovalis suggest shade acclimation. The red leaves of C. rotundata had lower xanthophyll content and de-epoxidation state of the xanthophyll cycle than the green leaves while the red leaves of H. ovalis contained higher xanthophyll content than the green leaves. The red leaves of C. rotundata displayed higher activity of ascorbate peroxidase and lower total reactive oxygen species, whereas, no significant difference in oxidative stress-related parameters between green and red leaves of H. ovalis was detected. Our results demonstrate that although anthocyanin appears to contribute to photoprotection by acting as sunscreen, it does not confer greater tolerance to high irradiance in H. ovalis and C. rotundata and anthocyanic seagrasses are not limited in their capacity for other photoprotective mechanisms. It is concluded that green-leafed and red-leafed seagrasses cope equally well with high light in their natural settings by utilizing different combinations of photoprotective mechanisms.
This project was financially supported by the Research Fund for DPST Graduates with First Placement Fiscal Year 2014 (Grant no. 16/2557) awarded by the Institute for the Promotion of Teaching Science and Technology (IPST). Special acknowledgment goes to Hadrien Verbois, Ekkalak Rattanachot and Sarit Songmuang for very helpful assistance during field trips. We also thank the project mentor, Assoc. Prof. Dr. Anchana Prathep. Finally, the authors wish to thank Dr. Alan Frederick-Geater for assistance with the English proofreading.
Compliance with ethical standards
Conflict of interest
Pimchanok Buapet has received research grant from IPST. The authors declare that there is no conflict of interest.
This article does not contain any studies with animals performed by any of the authors.
- Bischof K, Kräbs G, Wiencke C, Hanelt D (2002) Solar ultraviolet radiation affects the activity of ribulose-1,5-bisphosphate carboxylase-oxygenase and the composition of photosynthetic and xanthophyll cycle pigments in the intertidal green alga Ulva lactuca L. Planta 215:502–509CrossRefGoogle Scholar
- Dattolo E, Ruocco M, Brunet C, Lorenti M, Lauritano C, D’Esposito D, De Luca P, Sanges R, Mazzuca S, Procaccini G (2014) Response of the seagrass Posidonia oceanica to different light environments: insights from a combined molecular and photo-physiological study. Mar Environ Res 101:225–236CrossRefGoogle Scholar
- García-Sánchez M, Korbee N, Pérez-Ruzafa IM, Marcos C, Domínguez B, Figueroa FL, Pérez-Ruzafa A (2012) Physiological response and photoacclimation capacity of Caulerpa prolifera (Forsskål) J.V. Lamouroux and Cymodocea nodosa (Ucria) Ascherson meadows in the Mar Menor lagoon (SE Spain). Mar Environ Res 79:37–47CrossRefGoogle Scholar
- Jambunathan N (2010) Determination and detection of reactive oxygen species (ROS), lipid peroxidation, and electrolyte leakage in plants. Methods Mol Biol 639:292–298Google Scholar
- Kytridis VP, Karageorgou P, Levizou E, Manetas Y (2008) Intra-species variation in transient accumulation of leaf anthocyanins in Cistus creticus during winter: evidence that anthocyanins may compensate for an inherent photosynthetic and photoprotective inferiority of the red-leaf phenotype. J Plant Physiol 165:952–959CrossRefGoogle Scholar
- Marín-Guirao L, Ruiz JM, Sandoval-Gil JM, Bernardeau-Esteller J, Stinco CM, Meléndez-Martínez A (2013) Xanthophyll cycle-related photoprotective mechanism in the Mediterranean seagrasses Posidonia oceanica and Cymodocea nodosa under normal and stressful hypersaline conditions. Aquat Bot 109:14–24CrossRefGoogle Scholar
- Nakano Y, Asada K (1981) Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiol 22:867–880Google Scholar
- Platt T, Gallegos CL, Harrison WG (1980) Photoinhibition of photosynthesis in natural assemblages of marine phytoplankton. J Mar Res 38:687–701Google Scholar
- Procaccini G, Ruocco M, Marín-Guirao L, Dattolo E, Brunet C, D’Esposito D, Lauritano C, Mazzuca S, Serra IA, Bernardo L, Piro A, Beer S, Björk M, Gullström M, Buapet P, Rasmusson LM, Felisberto P, Gobert S, Runcie JW, Silva J, Olivé I, Costa MM, Barrote I, Santos R (2017) Depth-specific fluctuations of gene expression and protein abundance modulate the photophysiology in the seagrass Posidonia oceanica. Sci Rep 7:42890CrossRefGoogle Scholar
- Ragavan P, Saxena A, Mohan PM, Coomar T, Ragavan A (2013) Leaf reddening in seagrasses of Andaman and Nicobar Islands. Trop Ecol 54:269–273Google Scholar