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Marine Biology

, Volume 162, Issue 3, pp 687–697 | Cite as

Shell growth, microstructure and composition over the development cycle of the European abalone Haliotis tuberculata

  • S. Auzoux-BordenaveEmail author
  • C. Brahmi
  • A. Badou
  • M. de Rafélis
  • S. Huchette
Original Paper

Abstract

The shell of the European abalone Haliotis tuberculata is a model for studying mechanisms of mollusc shell formation, but the early steps of shell formation and calcification remain poorly documented. The microstructure and the mineralogical and geochemical composition of larval and juvenile shells were investigated by scanning electron microscopy, infrared spectroscopy and ion microprobe analyses (NanoSIMS). Analyses were performed on shells obtained from controlled fertilisations at the hatchery France-Haliotis (Plouguerneau, France) in July 2009 and 2010 using abalone from Roscoff. Shell cross sections revealed the microstructural arrangement of the developing shell, showing progressive biomineral organisation into two differentiated layers, i.e. the outer granular and the internal nacreous layer. Infrared analysis confirmed that the European abalone shell, at every stage of development, was mostly composed of CaCO3 in the form of aragonite. Variations in trace element composition, i.e. Sr/Ca, were measured in the different stages and correlated with micro-structural changes in the shells. Experimental manganese labelling of live abalones produced cathodoluminescence marks in the growing shell sections. The increase in shell thickness can be used to determine the growth rate of an early adult abalone shell.

Keywords

Trace Element Composition Shell Layer Shell Growth Larval Shell Nacreous Layer 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This work was financed in part by the ATM program “Biomineralisation” of the MNHN funded by the Ministère délégué à l’Enseignement Supérieur et à la Recherche (Paris, France). We thank Pr Anders Meibom for the facilities and assistance for the NanoSIMS analysis ion microprobe (Muséum national d’Histoire naturelle, Paris, France). We are grateful to Claire E. Lazareth (UMR LOCEAN, Bondy, France) for her kind reading of the manuscript.

Supplementary material

227_2015_2615_MOESM1_ESM.pdf (33 kb)
Supplementary material 1 (PDF 32 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • S. Auzoux-Bordenave
    • 1
    • 2
    Email author
  • C. Brahmi
    • 3
  • A. Badou
    • 1
  • M. de Rafélis
    • 4
  • S. Huchette
    • 5
  1. 1.UMR BOREA “Biologie des Organismes et Ecosystèmes Aquatiques”, MNHN/CNRS 7208/IRD 207/UPMCMuséum national d’Histoire naturelle, Station de Biologie Marine de ConcarneauConcarneauFrance
  2. 2.Sorbonne Universités, UPMCUniversité Paris 6ParisFrance
  3. 3.UMR EIOUniversité de Polynésie françaiseFaa’aFrench Polynesia
  4. 4.UMR 7193 “Biominéralisations et Environnements sédimentaires”UPMC-ISTePParisFrance
  5. 5.Ecloserie France-HaliotisPlouguerneauFrance

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