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Marine Biology

, Volume 161, Issue 8, pp 1883–1893 | Cite as

New genetic markers to identify European resistant abalone to vibriosis revealed by high-resolution melting analysis, a sensitive and fast approach

  • Anne-Leila MeistertzheimEmail author
  • Isabelle Calvès
  • Valérie Roussel
  • Alain Van Wormhoudt
  • Jean Laroche
  • Sylvain Huchette
  • Christine Paillard
Original Paper

Abstract

Increasing temperature of seawater is often associated with increased exposure incidence of disease in field and in aquaculture populations. Numerous episodic mass mortalities of the abalone Haliotis tuberculata have been observed along the northern Brittany coast of France caused by a complex interaction between the host, pathogen and environmental factors. Here, we evaluated the potential of high-resolution melting (HRM) analysis for mutation genotyping and development of genetic markers for resistance to vibriosis in the gastropod species H. tuberculata. Small amplicon assays were developed and revealed genetic polymorphism between surviving and susceptible abalone obtained after two successive infections of aquaculture families in controlled conditions. Together with specific COI haplotypes, we identified particular genotypes in nascent polypeptide-associated complex subunit alpha and ferritin genes linked to the susceptibility or resistance of abalone to vibriosis. Selection of genitors based on these genes may increase the proportion in offspring of resistant individuals of more than 76 %. Finally, HRM assays constitute a very efficient genotyping tool to validate the genetic markers on a representative number of individuals of wild populations and thus identify future resistant genitors for aquaculture or conservation purposes.

Keywords

Ferritin Vibrio FSSW Parentage Assignment Resistant Individual 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

The authors declare no competing financial interests and thank Jeanine Almany for correcting the English. The authors also acknowledge Emilie Boissin for her help for parentage assignment in COLONY. This research program was financially supported by (1) the European program SUDEVAB (Grant Agreement no 222 156), (2) a CPER—FEDER program for the sequencing part and (3) by the EVOLFISH program (financed by the National Agency for Research-VMCS, Paris, France).

Supplementary material

227_2014_2470_MOESM1_ESM.pdf (86 kb)
Supplementary material 1 (PDF 85 kb)
227_2014_2470_MOESM2_ESM.docx (19 kb)
Supplementary material 2 (DOCX 19 kb)
227_2014_2470_MOESM3_ESM.docx (14 kb)
Supplementary material 3 (DOCX 13 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Anne-Leila Meistertzheim
    • 1
    • 2
    Email author
  • Isabelle Calvès
    • 2
  • Valérie Roussel
    • 3
  • Alain Van Wormhoudt
    • 3
  • Jean Laroche
    • 2
  • Sylvain Huchette
    • 4
  • Christine Paillard
    • 2
  1. 1.Centre de Recherches Insulaires et Observatoire de l’Environnement de Polynésie Française (CRIOBE), USR 3278 CNRS/EPHE/UPVD, Laboratoire d’Excellence “CORAIL” Bâtiment CBETMUniversité de PerpignanPerpignan CedexFrance
  2. 2.Laboratoire des Sciences de l’Environnement Marin, UMR 6539 CNRS/UBO/IRD/Ifremer Institut Universitaire Européen de la MerUniversité de Bretagne OccidentalePlouzanéFrance
  3. 3.Station de Biologie Marine, UMR 7208 BOREAMuséum National d’Histoire NaturelleConcarneauFrance
  4. 4.France HaliotisPlouguerneauFrance

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