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Marine Biology

, Volume 160, Issue 10, pp 2699–2709 | Cite as

Bacteriolytic Bacillus species isolated from brackish waters of the Southern Baltic Sea

  • Christiane BrackEmail author
  • Annett Mikolasch
  • Rüdiger Pukall
  • Peter Schumann
  • Marion Köster
  • Frieder Schauer
Original Paper

Abstract

The present study examines antagonistic relationships between different microorganisms inhabiting brackish water and includes a systematic screening for bacteriolytic prokaryotes in the brackish waters of the Southern Baltic Sea (Nordruegenscher Bodden) sampled in July/August 2009. Ten of the 35 marine bacteriolytic isolates belong to the genus Bacillus. Five isolates (B. pumilus, B. subtilis, B. megaterium, B. licheniformis) lysed living microbial cells such as the bacteria Arthrobacter citreus, Arthrobacter protophormiae, Micrococcus luteus, Pseudomonas putida and the yeast Trichosporon mucoides. These and other bacteria (Aeromonas sp., Bacillus subtilis, Chromobacterium violaceum, Citrobacter freundii, Enterobacter aerogenes, Proteus mirabilis, Pseudomonas aeruginosa and Serratia marcescens) were also lysed as autoclaved and pasteurized cells on agar plates. One isolate of Bacillus pumilus showed a distinct bacteriolysis activity against pasteurized cells of A. citreus in liquid culture. Our results suggest that Bacillus species may play a role as opportunistic predators in the marine microbial food web.

Keywords

Arthrobacter Bacillus Species Cellular Fatty Acid Profile Prey Cell Lysis Activity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

The authors thank Anne Reinhard, Brigitte Fricke, Gaby Pötter and Ulrike Steiner for laboratory assistance, Bob Jack for reviewing the manuscript and Sabine Schade (Institute of Microbiology, Greifswald) for the photography of lysis zones. We also thank the government of Mecklenburg-Vorpommern (Germany) for financial support in the form of Landesgraduiertenstipendium.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Christiane Brack
    • 1
    Email author
  • Annett Mikolasch
    • 1
  • Rüdiger Pukall
    • 2
  • Peter Schumann
    • 2
  • Marion Köster
    • 3
  • Frieder Schauer
    • 1
  1. 1.Department of Applied Microbiology, Institute of MicrobiologyUniversity GreifswaldGreifswaldGermany
  2. 2.Leibniz Institute DSMZ—German Collection of Microorganisms and Cell CulturesBraunschweigGermany
  3. 3.Department of Microbial Ecology, Institute of MicrobiologyUniversity GreifswaldInsel HiddenseeGermany

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