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Marine Biology

, Volume 160, Issue 10, pp 2547–2560 | Cite as

Differential effect of ultraviolet exposure (UVR) in the stress response of the Dinophycea Gymnodinium sp. and the Chlorophyta Dunaliella tertiolecta: mortality versus survival

  • Josée Nina BouchardEmail author
  • Candela García-Gómez
  • M. Rosario Lorenzo
  • María SegoviaEmail author
Original Paper

Abstract

Dunaliella tertiolecta (Chlorophyta) and Gymnodinium sp. (Dinophyceae) cells were exposed to ultraviolet radiation (UVR) (PAR, UVA and UVB: PAB) for 6 days either continuously or during a photoperiod. Both UVR treatments were harmful to Gymnodinium but exposure to continuous PAB had the most dramatic effects. Although a number of lesions/damage could have happened during the first few hours of exposure to UVR, in less than 24 h, Gymnodinium lost its ability to detoxify ROS efficiently, photoinhibition occurred, thymine dimers formed in the DNA, caspase-like enzymatic activities DEVDase sharply increased and cells died as determined by SYTOX-green staining. Superoxide dismutase activity did not significantly change with time, and although the catalase activity augmented in both treatments, cells still suffered from the UVR stress. Clearly, UVR was fatal to the dinoflagellate. For the chlorophyte, however, cell numbers increased regardless of the UVR treatment and mortality remained low (<20 %). F v/F m showed an initial decrease but then remained constant for both light treatments. After 6 days of continuous PAB exposure, however, signs of stress (thymine dimers, oxidative stress) paralleled a drop in catalase activity. Results obtained here demonstrate that the dinoflagellate Gymnodinium was much more sensitive and was harmed more rapidly by UVR exposure than the chlorophyte D. tertiolecta. The increased tolerance to UVR exposure of the chlorophyte may provide advantages over other more sensitive phytoplankton species within the photic zone. We provide strong support in the present study for repair being an important component of UV resistance in this species.

Keywords

Reactive Oxygen Species Dinoflagellate Catalase Activity Light Treatment PSII Reaction Centre 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

The present work was supported by Research Grants CTM/MAR10-17216 from the Ministry for Science and Innovation (MICCIN) and P08-03800 from the Regional Science Research Programme (Junta de Andalucía) Spain to MS. JNB was funded to carry out a short-stay postdoctoral-grant at MS Laboratory by the Regional Science Research Programme (Junta de Andalucía, Spain). CG and MRL were funded by PhD grants associated to the Research Grants mentioned above. We are grateful to Patrick Neale for thorough discussions and suggestions.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  1. 1.National Oceanography CentreUniversity of SouthamptonSouthamptonUK
  2. 2.Department of Ecology, Faculty of SciencesUniversity of MálagaMálagaSpain
  3. 3.Algenol BiofuelsFort MyersUSA

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