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Identification of sperm proteins as candidate biomarkers for the analysis of reproductive isolation in Mytilus: a case study for the enkurin locus

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Abstract

Sperm proteins of the marine sessile mussels of the Mytilus edulis species complex are models to investigate reproductive isolation and speciation. This study aimed at identifying sperm proteins and their corresponding genes. This was aided by the use of monoclonal antibodies that preferentially bind to yet unknown sperm molecules. By identifying their target molecules, this approach identified proteins with relevance to Mytilus sperm function. This procedure identified 16 proteins, for example, enkurin, laminin, porin and heat shock proteins. The potential use of these proteins as genetic markers to study reproductive isolation is exemplified by analysing the enkurin locus. Enkurin evolution is driven by purifying selection, the locus displays high levels of intraspecific variation and species-specific alleles group in distinct phylogenetic clusters. These findings characterize enkurin as informative candidate biomarker for analyses of clinal variation and differential introgression in hybrid zones, for example, to understand determinants of reproductive isolation in Baltic Mytilus populations.

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Acknowledgments

We thank Karina Roth (University Potsdam, Germany) and Dr. Marius Ader (DFG-Center for Regenerative Therapies Dresden, Dresden University of Technology, Germany) for support in performing protein analysis. The project was supported by Deutsche Forschungsgemeinschaft (DFG, STU 519/2-1) and Paul Ungerer-Stiftung (Frankfurt/Main, Germany).

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Authors and Affiliations

  1. Senckenberg Dresden, Museum of Zoology (Museum für Tierkunde), Königsbrücker Landstrasse 159, 01109, Dresden, Germany

    Manuela Bartel & Heiko Stuckas

  2. Unit of Bioinformatics, Institute of Biochemistry and Biology, University of Potsdam, Karl-Liebknecht Strasse 24-25, 14476, Potsdam, Germany

    Stefanie Hartmann

  3. Proteome Factory AG, Magnusstrasse 11, 12498, Berlin, Germany

    Karola Lehmann

  4. DFG-Center for Regenerative Therapies Dresden, Retinal Regeneration, Dresden University of Technology, Cluster of Excellence, Fetscherstrasse 105, 01307, Dresden, Germany

    Kai Postel

  5. Departamento de Bioquímica, Genética e Immunología, Facultad de Biología, Universidad de Vigo, Vigo, Spain

    Humberto Quesada

  6. Institute of Clinical Molecular Biology, Christian-Albrechts-University Kiel, Universitätsklinikum Schleswig Holstein, Campus Kiel, Schittenhelmstr. 12, 24105, Kiel, Germany

    Eva E. R. Philipp

  7. Junior Research Group Antibody Technology, Institute of Biochemistry and Biology, University of Potsdam, Karl-Liebknecht Strasse 24-25, 14476, Potsdam, Germany

    Katja Heilmann & Burkhard Micheel

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  1. Manuela Bartel
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  2. Stefanie Hartmann
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  3. Karola Lehmann
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  4. Kai Postel
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  6. Eva E. R. Philipp
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  8. Burkhard Micheel
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  9. Heiko Stuckas
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Corresponding author

Correspondence to Heiko Stuckas.

Additional information

Communicated by T. Reusch.

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Bartel, M., Hartmann, S., Lehmann, K. et al. Identification of sperm proteins as candidate biomarkers for the analysis of reproductive isolation in Mytilus: a case study for the enkurin locus. Mar Biol 159, 2195–2207 (2012). https://doi.org/10.1007/s00227-012-2005-7

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  • DOI: https://doi.org/10.1007/s00227-012-2005-7

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