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Biomineralization markers during early shell formation in the European abalone Haliotis tuberculata, Linnaeus

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Abstract

Larval shell formation was investigated in the European abalone Haliotis tuberculata. Stages of mineralization as well as enzymatic and endocrine biomarkers were monitored throughout larval development, from hatching to post-larval stages. Polarized light microscopy and infrared spectroscopy analyses revealed the presence of crystallized calcium carbonate arranged in aragonite polymorphs from the late trochophore stage. A correlation between the main steps of shell formation and enzymatic activities of alkaline phosphatase and carbonic anhydrase was seen. The variations of these biologic activities were related to the onset of mineralization, the rapid shell growth, and the switch from larval to juvenile shell following metamorphosis. Furthermore, a strong increase in the level of calcitonin gene-related molecules was measured in post-larvae, suggesting that endocrine control takes place after metamorphosis. The changes measured for the three biomineralization markers together with mineralogical analysis allowed us to correlate physiologic mechanisms with early steps of abalone shell formation.

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Acknowledgments

We thank B. Marie, F. Marin (University of Bourgogne, Dijon, France), C. Milet (Muséum National d’Histoire Naturelle, Paris, France), and J.-Y. Sire (University of Paris 6) for helpful discussions. We also thank S. Thompson (University of Keele, UK) for the English corrections. FTIR analyses were carried out at the Muséum National d’Histoire Naturelle; we thank Pr. F. Fröhlich (MNHN) for help in FTIR spectra interpretation. This work was supported in part by the European Community: The Atlantic Area Programme BIOTECMAR n° 2008-1/032 and by the Programme PluriFormations “Biomineralization”. B. Gaume was financed by a PhD fellowship from the Ministère de l’Enseignement Supérieur et de la Recherche, in France. The experiments complied with the current French laws.

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Authors and Affiliations

  1. UMR BOREA (Biologie des Organismes et Ecosystèmes Aquatiques), MNHN/CNRS 7208/IRD 207/UPMC, Muséum national d’Histoire naturelle, Station de Biologie Marine, 29900, Concarneau, France

    Béatrice Gaume, Martine Fouchereau-Peron, Marie-Noëlle Helléouet & Stéphanie Auzoux-Bordenave

  2. UMR 7194/USM 204, Centre de Spectroscopie Infrarouge, Département Préhistoire, Muséum National d’Histoire Naturelle, 43 rue Buffon, 75005, Paris, France

    Aïcha Badou

  3. Ecloserie France-Haliotis, Kérazan, 29880, Plouguerneau, France

    Sylvain Huchette

  4. Université Pierre et Marie Curie Paris VI, 4 place Jussieu, 75005, Paris, France

    Stéphanie Auzoux-Bordenave

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  1. Béatrice Gaume
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  2. Martine Fouchereau-Peron
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  6. Stéphanie Auzoux-Bordenave
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Correspondence to Béatrice Gaume.

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Communicated by H. O. Pörtner.

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Gaume, B., Fouchereau-Peron, M., Badou, A. et al. Biomineralization markers during early shell formation in the European abalone Haliotis tuberculata, Linnaeus. Mar Biol 158, 341–353 (2011). https://doi.org/10.1007/s00227-010-1562-x

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