Abstract
This study represents the first determination of lipids and fatty acids in fish scales. Scales collected from groups of Atlantic salmon reared on fish farms and in experimental tanks were analyzed by chromatography. The complete suite of fatty acids normally found in marine organisms was detected in the scales, with the following fatty acids dominating: 16:0, 18:0, 18:1n9, 20:5n3, 22:6n3 and 24:1n9. Scales contained relatively high levels of furan fatty acids, and the level of cholesterol (2.5–5 mg/g tissue) was much higher than the levels found in the edible parts of marine fishes (0.2–1 mg/g tissue). The fatty acid profile of scales was distinct between groups of salmon originating from different commercial strains reared on the same farm, between salmon groups originating from the same strains but reared at different farms, and between groups of fed and unfed salmon in experimental tanks. Together, these data indicate that the fatty acid composition of fish scales is dependent upon both environmental and genetic factors. The fatty acid composition of fish scales may be used in stock/population identification, for example identification of escaped Atlantic salmon to farm of origin.
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References
Brosche T, Platt D, Vostorowsky O (1985) Methyl enol ethers as artefacts in capillary gas chromatographic profiles of aldehydes dimethyl acetals. J Chromatog 345:219–227
Crozier WW (1993) Evidence of genetic interaction between escaped farmed salmon and wild Atlantic salmon (Salmo salar L.) in a northern Irish river. Aquaculture 113:19–29
Dalsgaard J, St. John M, Kattner G, Müller-Navarra D, Hagen W (2003) Fatty acid trophic markers in the pelagic marine environment. Advan Mar Biol 46:225–340
Glover KA (2008) Genetic characterisation of farmed rainbow trout in Norway: intra- and inter-strain variation reveals potential for genetic assignment of escapees. BMC Genet 9:87
Glover KA, Skilbrei OT, Skaala Ø (2008) Genetic assignment identifies farm of origin for Atlantic salmon Salmo salar escapees in a Norwegian fjord. ICES J Mar Sci 65:912–920
Glover KA, Hansen MM, Skaala Ø (2009) Identifying the source of farmed escaped Atlantic salmon (Salmo salar): Bayesian clustering analysis increases accuracy of assignment. Aquaculture 290:37–46
Grahl-Nielsen O (2005) Fatty acid profiles as natural marks for stock identification. In: Cadrin SX, Friedland KD, Waldman JR (eds) Stock identification methods. Academic Press, New York, pp 239–261
Hindar K, Fleming IA, McGinnity P, Diserud A (2006) Genetic and ecological effects of salmon farming on wild salmon: modelling from experimental results. ICES J Mar Sci 63:1234–1247
Kellogg KA (1999) Salmon on the edge. Trends Ecol Evol 14:45–46
Kvalheim OM, Karstang TV (1987) A General purpose Program for Multivariate Data Analysis. Chemom Intell Lab Syst 2:235–237
Lund RA, Hansen LP (1991) Identification of released and wild Atlantic salmon. Salmo salar L., using scale characters. Aquacult Fish Managem 22:499–508
Meier S, Mjøs S, Joensen H, Grahl-Nielsen O (2006) Validation of a one-step extraction/methylation method for determination of fatty acids and cholesterol in marine tissues. J Chromatog A 1104:291–298
Mjaavatten O, Levings CD, Poon P (1998) Variation in the fatty acid composition of juvenile chinook and coho salmon from Fraser river estuary determined by multivariate analysis; role of environment, and genetic origin. Comp Biochem Physiol B 120:291–309
Napolitano GE, MacDonald BA, Thompson RJ, Ackman RG (1992) Lipid composition of eggs and adductor muscle in giant scallops (Placopecten magellanicus) from different habitats. Mar Biol 113:71–76
Oehlenschläger J (2006) Cholesterol content in seafood, data from the last decade: a review. In: Luten LB, Jacobsen C, Bekaert K, Saebø A, Oehlenschläger J (eds) Seafood research from fish to dish. Wageningen Academic Publishers, Wageningen, pp 41–57
Okada Y, Kaneko M, Okajima H (1996) Hydroxy radical scavenging activity of naturally occurring furan fatty acids. Biol Pharmaceut Bull 19:1607–1610
Olsen Y (1999) Lipids and essential fatty acids in aquatic food webs: what can freshwater ecologists learn from mariculture? In: Arts MT, Wainman BC (eds) Lipids in freshwater ecosystems. Springer, New York, pp 161–202. ISBN 0-387-98505-0
Parrish DL, Behnke RJ, Gephard SR, McCormick SD, Reeves GH (1998) Why aren′t there more Atlantic salmon (Salmo salar)? Can J Fish Aquat Sci 55:281–287
Robertson JC, Hazel JR (1999) Influence of temperature and membrane lipid composition on the osmotic water permeability of teleost gills. Physiol Biochem Zool 72:623–632
Spiteller G (2005) Furan fatty acids: occurence, synthesis, and reactions. Are furan fatty acids responsible for the cardioprotective effects of a fish diet? Lipids 40:755–771
Velansky PV, Kostetsky EY (2008) Lipids of marine cold-water fishes. Russian J Marine Biol 34:51–56
Wold S (1978) Pattern recognition by means of disjoint principal component models. Pattern Recogn 8:127–139
WWF (2001) The status of wild Atlantic salmon: A river by river assessment. Available at http://www.wwf.org.uk/filelibrary/pdf/atlanticsalmon.pdf
Acknowledgments
Rolf Haugarvoll from Lingalaks A. S. is gratefully acknowledged for permitting access to sample scales from salmon on their farms. Ivar-Helge Matre is acknowledged for assisting in setting up the stability experiment at the Matre field station. We thank Morten Tysnes Olstad and Marit Bøe Vaage for assistance in laboratory work, and Dr. Svein Are Mjøs for helping in identification of the furan FAs. The stability experiment was conducted with permission from the Norwegian animal experimentation committee. This study was conducted with finances from a Norwegian Research Council project (project no. 172628/S40) managed by Dr. Øystein Skaala.
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Communicated by M. A. Peck.
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Grahl-Nielsen, O., Glover, K.A. Fatty acids in fish scales. Mar Biol 157, 1567–1576 (2010). https://doi.org/10.1007/s00227-010-1430-8
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DOI: https://doi.org/10.1007/s00227-010-1430-8