Skip to main content

Advertisement

SpringerLink
Log in

Molecular phylogeography of two Italian sibling species of Calobius (Coleoptera, Hydraenidae, Ochthebiinae) inhabiting Mediterranean marine rock-pools

  • Original Paper
  • Published:
Marine Biology Aims and scope Submit manuscript

Abstract

Marine rock-pools, commonly found along the Mediterranean coasts, are isolated patches of habitat characterised by large spatial and temporal variations. The phylogeography of Calobius quadricollis and C. urbanelliae, two sibling species of moss beetles (Coleoptera, Hydraenidae) inhabiting Italian temporary marine rock-pool ecosystems, were studied using mitochondrial cytochrome c oxidase subunit I (COI). Our data suggest a strong association between the scored genetic variability and the geographical distribution of populations sampled for both species. These analyses provided evidences of episodic species range expansion and fragmentation, recurrent “flush and crash” and bottleneck episodes probably occurring during the Glacial Cycles. The observed phylogeographical pattern is probably related to the historical and biogeographical processes of Mediterranean areas, as well as both to the ephemeral habitat tipology and limited dispersal ability of these beetles.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  • Abellàn P, Gòmez-Zurita J, Millàn A et al (2007) Conservation genetics in hypersaline inland waters: mitochondrial diversity and phylogeography of an endangered Iberian beetle (Coleoptera: Hydraenidae). Conserv Gen 8:79–88

    Article  Google Scholar 

  • Astles KL (1993) Patterns of abundance and distribution of species in intertidal rock pools. J. Mar Biol Ass UK 13:555–569

    Article  Google Scholar 

  • Audisio P (1984) I Kateretidae e i Nitidulidae (Coleoptera) dell’Italia meridionale: distribuzione ed ipotesi sul popolamento. Biogeographia 10:605–623

    Google Scholar 

  • Audisio P, Trizzino M, De Biase A, Mancini E, Antonini G (2009) Molecular and morphological evidence of a new sibling species of Calobius (Coleoptera: Hydraenidae) of the C. quadricollis complex from peninsular Italy. Italian J Zool. doi:10.1080/11250000902845738

    Google Scholar 

  • Bonfield J, Beal K, Jordan M, Cheng Y, Staden R (2005) The Staden Package Manual. Medical Research Council, Laboratory of Molecular Biology, Cambridge

    Google Scholar 

  • Bonfiglio L, Mangano G, Marra AC, Masini F, Pavia M, Petruso D (2002) Pleistocene calabrian and sicilian bioprovinces. Geobios 35:29–39

    Article  Google Scholar 

  • Burton RS (1997) Genetic evidence for persistence of marine invertebrate populations in an ephemeral environment. Evolution 51:993–998

    Article  Google Scholar 

  • Burton RS, Lee B-N (1994) Nuclear and mitochondrial gene geneologies and allozyme polymorphism across a major phylogeographic break in the copepod Tigriopus californicus. Proc Nat Acad Sci USA 91:5197–5201

    Article  CAS  PubMed  Google Scholar 

  • Canestrelli D, Nascetti G (2008) Phylogeography of the pool frog Rana (Pelophylax) lessonae in the Italian peninsula and Sicily: multiple refugia, glacial expansions and nuclear–mitochondrial discordance. J Biogeogr 35:1923–1936

    Article  Google Scholar 

  • Canestrelli D, Cimmaruta R, Costantini V, Nascetti G (2006) Genetic diversity and phylogeography of the apennine yellow-bellied toad Bombina pachypus (Anura: Discoglossidae), with implication for conservation. Mol Ecol 15(12):3741–3754

    Article  CAS  PubMed  Google Scholar 

  • Charlesworth B, Charlesworth D, Barton NH (2003) The effects of genetic and geographic structure on neutral variation. Annu Rev Ecol Evol S 34:99–125

    Article  Google Scholar 

  • Clement M, Posada D, Crandall KA (2000) TCS: a computer program to estimate gene genealogies. Mol Ecol 9:1657–1659

    Article  CAS  PubMed  Google Scholar 

  • Denis F, Ravallec L, Pavillon J, Wormhoudt AV (2009) Genetic differentiation of Atlantic populations of the intertidal copepod Tigriopus brevicornis. Sci Mar 73:579–587

    Article  CAS  Google Scholar 

  • Edmands S (2001) Phylogeography of the intertidal copepod Tigriopus californicus reveals substantially reduced population differentiation at northern latitudes. Mol Ecol 10:1743–1750

    Article  CAS  PubMed  Google Scholar 

  • Excoffier L, Smouse PE, Quattro JM (1992) Analysis of molecular variance inferred from metric distances among DNA haplotypes: Application to human mitochondrial DNA restriction data. Genetics 131:479–491

    CAS  PubMed  Google Scholar 

  • Excoffier L, Laval G, Schneider S (2005) Arlequin ver. 3.0: An integrated software package for population genetics data analysis. Evol Bioinform Online 1:47–50

    CAS  PubMed  Google Scholar 

  • Fu YX (1997) Statistical tests of neutrality of mutations against population growth, hitchhiking and background selection. Genetics 147:915–925

    CAS  PubMed  Google Scholar 

  • Fukatsu T (1999) Acetone preservation: a practical technique for molecular analysis. Mol Ecol 8:1935–1945

    Article  CAS  PubMed  Google Scholar 

  • Ganning B (1967) Laboratory experiments in the ecological work on rockpool animals with special notes on the ostracod Heterocypris salinus. Helgolan Wiss Meer 15:27–40

    Article  Google Scholar 

  • Ganning B (1970) Population dynamics and salinity tolerance of Hyadesia fusca (Lohman) (Acarina, Sarcoptiformes) from brackish water rockpools, with notes on the microenvironment inside Enteromorpha tubes. Oecologia 5:127–137

    Article  Google Scholar 

  • Ganning B (1971) On the ecology of Heterocypris salinus, H. incongruens and Cypridopsis aculeate (Crustacea : Ostracoda) from Baltic brackish-water rockpools. Mar Biol 8:271–279

    Article  Google Scholar 

  • Giraudi C (2004) The Apennine glaciations in Italy. In: Ehlers J, Gibbard PL (eds) Quaternary glaciations—extent and chronology, part I: Europe. Developments in quaternary science, vol 2. Elsevier, Amsterdam

    Chapter  Google Scholar 

  • Gómez A, Lunt DH (2007) Refugia within refugia: patterns of phylogeographic concordance in the Iberian Peninsula. In: Weiss S, Ferrand N (eds) Phylogeography of Southern European Refugia. Springer, London, pp 155–188

    Chapter  Google Scholar 

  • Hewitt GM (1996) Some genetic consequences of ice ages and their role in divergence and speciation. Biol J Linn Soc 58:247–276

    Google Scholar 

  • Hewitt GM (1999) Postglacial re-colonization of European biota. Biol J Linn Soc 68:87–112

    Article  Google Scholar 

  • Hewitt GM (2000) The genetic legacy of the quaternary ice ages. Nature 405:907–913

    Article  CAS  PubMed  Google Scholar 

  • Hewitt GM (2001) Speciation, hybrid zones and phylogeography—or seeing genes in space and time. Mol Ecol 10:537–549

    Article  CAS  PubMed  Google Scholar 

  • Hewitt GM (2004) Genetic consequences of climatic oscillations in the quaternary. Phil Trans R Soc B 359:183–195

    Article  CAS  PubMed  Google Scholar 

  • Hulsmans A, Vanschoenwinkel B, Pyke C, Riddoch BJ, Brendonck L (2008) Quantifying the hydroregime of a temporary pool habitat: a modelling approach for ephemeral rock pools in SE Botswana. Ecosystems 11:89–100

    Article  Google Scholar 

  • Jäch M (1993) Revision of the paleartic species of genus Ochthebius XI. The subgenus Calobius Wollaston, 1854 (Insecta: Coleoptera: Hydraenidae). Reichenbachia 30(7):33–45

    Google Scholar 

  • Knowles LL, Maddison WP (2002) Statistical phylogeography. Mol Ecol 11(12):2623–2635

    Article  PubMed  Google Scholar 

  • Liggins L, Chapple DG, Daugherty CH, Ritchie PA (2008) Origin and post-colonization evolution of the Chatham Islands skink (Oligosoma nigriplantare nigriplantare). Mol Ecol 17:3290–3305

    Article  CAS  PubMed  Google Scholar 

  • Loder TC III, Ganning B, Love JA (1996) Ammonia nitrogen dynamics in coastal rockpools affected by gull guano. J Exp Mar Biol Ecol 196:113–129

    Article  Google Scholar 

  • Metaxas A, Scheibling RE (1993) Community structure and organization of tidepools. Mar Ecol Prog Ser 98:187–198

    Article  Google Scholar 

  • Panchal M, Beaumont M (2007) The automation and evaluation of nested clade phylogeographic analysis. Evolution 61(6):1466–1480

    Article  PubMed  Google Scholar 

  • Petit RJ (2008) On the falsifiability of the nested clade phylogeographic analysis method. Mol Ecol 17:1404

    Article  Google Scholar 

  • Pignatti S (1984) La flora dell’Appennino meridionale: distribuzione attuale ed ipotesi sull’origine. Biogeographia 10:89–100

    Google Scholar 

  • Posada D, Templeton AR (2004) GeoDis: differentiating population structure from history, version 2.2. Brigham Young University, Provo

    Google Scholar 

  • Ramos-Onsins SE, Rozas J (2002) Statistical properties of new neutrality tests against population growth. Mol Biol Evol 19:2092–2100

    CAS  PubMed  Google Scholar 

  • Ranta E (1982) Animal communities in rockpools. Ann Fenn Zool 19:337–348

    Google Scholar 

  • Rawson PD, Burton RS (2006) Molecular evolution at the cytochrome oxidase subunit 2 gene among divergent populations of the intertidal copepod, Tigriopus californicus. J Mol Evol 62:753–764

    Article  CAS  PubMed  Google Scholar 

  • Rozas J, Sanchez-Delbarrio JC, Messeguer X, Rozas R (2003) DNAsp, DNA polymorphism analyses by the coalescent and other methods. Bioinformatics 19:2496–2497

    Article  CAS  PubMed  Google Scholar 

  • Sambrook J, Russell D (2000) Molecular cloning. A laboratory manual, 3rd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, p 999

    Google Scholar 

  • Santucci F, Nascetti G, Bullini L (1996) Hybrid zones between two genetically differentiated forms of the pond frog Rana lessonae in southern Italy. J Evol Biol 9:429–450

    Article  Google Scholar 

  • Sbordoni V, Allegrucci G, Todiscio V (2004) Il genere Dolichopoda in Sardegna: filogenesi molecolare e ipotesi sulla evoluzione del popolamento. Studi Trentini Sci Nat Acta Biol 81:103–111

    Google Scholar 

  • Simon C, Frati F, Beckenbach A et al (1994) Evolution, weighting and phylogenetic utility of mitochondrial gene sequences and a compilation of conserved polymerase chain reaction primers. Ann Entomol Soc Am 87:1–51

    Google Scholar 

  • Taberlet P, Fumagalli L, Wust-Saucy AG, Cosson JF (1998) Comparative phylogeography and postglacial colonization routes in Europe. Mol Evol 7:453–464

    CAS  Google Scholar 

  • Tajima F (1989) Statistical method for testing the neutral mutation hypothesis by DNA polymorphism. Genetics 123:585–595

    CAS  PubMed  Google Scholar 

  • Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol 24:1596–1599

    Article  CAS  PubMed  Google Scholar 

  • Templeton AR (1995) A cladistic analysis of phenotype association with haplotypes inferred from restriction endonuclease mapping or DNA sequencing. V. Analysis of case/control sampling designs: Alzheimer’s disease and the apoprotein E locus. Genetics 140:403–409

    CAS  PubMed  Google Scholar 

  • Templeton AR (1998) Nested clade analysis of phylogeographyc data: testing hypotheses about gene flow and population history. Mol Ecol 7:381–397

    Article  CAS  PubMed  Google Scholar 

  • Templeton AR (2001) Using phylogeographic analyses of gene trees to test species status and processes. Mol Ecol 10:779–791

    Article  CAS  PubMed  Google Scholar 

  • Templeton AR (2004) Statistical phylogeography: methods of evaluating and minimizing inference errors. Mol Ecol 13:789–809

    Article  PubMed  Google Scholar 

  • Templeton AR (2008) Nested clade analysis: an extensively validated method for strong phylogeographic inference. Mol Ecol 17:1877–1880

    Article  PubMed  Google Scholar 

  • Templeton AR, Sing CF (1993) A cladistic analysis of phenotype association with haplotypes inferred from restriction endonuclease mapping. IV. Nested analysis with cladogram uncertainty and recombination. Genetics 134:659–669

    CAS  PubMed  Google Scholar 

  • Templeton AR, Boerwinkle E, Sing CF (1987) A cladistic analysis of phenotype association with haplotypes inferred from restriction endonuclease mapping. I. Basic theory and analysis of alcohol dehydrogenase activity in Drosophila. Genetics 117:343–351

    CAS  PubMed  Google Scholar 

  • Templeton AR, Sing CF, Kessling A, Humphries S (1988) A cladistic analysis of phenotype association with haplotypes inferred from restriction endonuclease mapping. II. The analysis of natural populations. Genetics 120:1145–1154

    CAS  PubMed  Google Scholar 

  • Templeton AR, Crandall KA, Sing CF (1992) A cladistic analysis of phenotype association with haplotypes inferred from restriction endonuclease mapping or DNA sequence data. III. Cladogram estimation. Genetics 132:619–633

    CAS  PubMed  Google Scholar 

  • Underwood AJ, Skilleter GA (1996) Effects of patch-size on the structure of assemblages in rock pools. J Exp Mar Biol Ecol 197:63–90

    Article  Google Scholar 

  • Urbanelli S (2002) Genetic divergence and reproductive isolation in the Ochthebius (Calobius) complex (Coleoptera: Hydraenidae). Heredity 88:333–341

    Article  CAS  PubMed  Google Scholar 

  • Urbanelli S, Porretta D (2008) Evidence of reinforcement of premating isolation between two species of the genus Ochthebius (Coleoptera: Hydraenidae). Evolution 62:1520–1527

    Article  PubMed  Google Scholar 

  • Urbanelli S, Sallicandro P, De Vito E, Colonnelli E, Bullini L (1996) Molecular re-examination of the taxonomy of Ochthebius (Calobius) (Coleoptera: Hydraenidae) from the Mediterranean and Macaronesian Regions. Ann Entomol Soc Am 89:623–636

    CAS  Google Scholar 

  • Willett CS, Ladner JT (2009) Investigations of fine-scale phylogeography in Tigriopus californicus reveal historical patterns of population divergence. BMC Evol Biol 9:139

    Article  PubMed  Google Scholar 

Download references

Acknowledgments

This paper was supported by M.I.U.R.–“University of Rome La Sapienza 60% funds” (Grant “Aspetti genetici e morfometrici della biodiversità animale in aree africane e medio-orientali a basso impatto antropico”). We are indebted with Daniel Whitmore (Centro Nazionale per la Biodiversità Forestale, Verona, Italy) and Marie-Claude Bon (European Biological Control Laboratories, Montpellier, France) for their helpful comments and valuable suggestions on early draft of the paper. We would also to thank our friend Giorgia Coletti for her help in collecting beetles in Southern Italy, Paolo Colangelo and Roberto Feuda (Italy, Rome) for their precious help and suggestions with statistical analyses.

Author information

Authors and Affiliations

  1. Dipartimento di Biologia Animale e Dell’Uomo, ‘Sapienza’, Università di Roma, Via A. Borelli 50, 00185, Rome, Italy

    Gloria Antonini, Paolo Audisio, Alessio De Biase, Giulia Rossetti & Marco Trizzino

  2. Dipartimento di Scienze di Sanità Pubblica, ‘Sapienza’, Università di Roma, Piazzale Aldo Moro 5, 00185, Rome, Italy

    Emiliano Mancini

  3. C. R. ENEA, La Casaccia, Via Anguillarese 301, 00060, S. M. di Galeria, Roma, Italy

    Carlo Tronci

Authors
  1. Gloria Antonini
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Paolo Audisio
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Emiliano Mancini
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Alessio De Biase
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Carlo Tronci
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Giulia Rossetti
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Marco Trizzino
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Marco Trizzino.

Additional information

Communicated by S. Uthicke.

Electronic supplementary material

Below is the link to the electronic supplementary material.

(DOC 57 kb)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Antonini, G., Audisio, P., Mancini, E. et al. Molecular phylogeography of two Italian sibling species of Calobius (Coleoptera, Hydraenidae, Ochthebiinae) inhabiting Mediterranean marine rock-pools. Mar Biol 157, 371–381 (2010). https://doi.org/10.1007/s00227-009-1324-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00227-009-1324-9

Keywords

Search

Navigation