Epithelial immunity in a marine invertebrate: a cytolytic activity from a cuticular secretion of the American horseshoe crab, Limulus polyphemus
The cuticle of the American horseshoe crab, Limulus polyphemus, is largely free of the macroscopic epibionts that rapidly colonize most solid surfaces exposed to sea water. Here, we describe a viscous surface secretion that coats the carapace of the horseshoe crab. We report methods for stimulating production of the substance, identify hemolytic and liposome-permeabilizing activities and provide a partial biochemical characterization. We propose that this secretion functions as an anti-fouling agent protecting the chitinized epithelium of the cuticle from colonization by deleterious epibionts.
KeywordsHemolytic Activity Hemocyte Horseshoe Crab Cytolytic Activity Marine Biological Laboratory
This research was supported by grant 0344360 from the National Science Foundation (to P.B.A.) and SFB 617 from the German Research Council (to M.L.). All of the experiments performed in this study comply with the current laws of the United States of America.
- Armstrong PB (2003) Internal defense against pathogenic invasion: the immune system. In: Shuster CN, Barlow RB, Brockman HJ (eds) The American horseshoe crab. Harvard University Press, Cambridge, pp 288–309Google Scholar
- Fahrenbach WH (1999). In: Harrison FW, Foelix RR (eds) Microscopic anatomy of invertebrates: chelicerate arthropoda. Wiley-Liss, New York, pp 21–115Google Scholar
- Grant D (2001) Living on Limulus. In: Tanacredi JT (ed) Limulus in the limelight: a species 350 million yeas in the Makingand in peril? Kluwer Academic/Plenum Publishers, New York, pp 135–145Google Scholar
- Harlow E, Lane D (1988) Anitbodies: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring HarborGoogle Scholar
- Law SKA, Reid KBM (1995) Complement. Oxford University Press, New YorkGoogle Scholar
- Leibovitz L, Lewbart GA (2003) Diseases and symbionts: vulnerability despite tough shells. In: Shuster CN, Barlow RB, Brockman HJ (eds) The American horseshoe crab. Harvard University Press, Cambridge, pp 245–237Google Scholar
- Shuster CN, Sekiguchi K (2003) Growing up takes about ten years and eighteen stages. In: Shuster CN, Barlow RB, Brockman HJ (eds) The American horseshoe crab. Harvard University Press, Cambridge, pp 103–132Google Scholar
- Stagner JI, Redmond JR (1975) The immunological mechanisms of the horseshoe crab, Limulus polyphemus. Mar Fish Rev 37:11–19Google Scholar