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Superoxide dismutase in the marine sponge Cliona celata

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Abstract

The aim of this work is to investigate the activity of the antioxidant enzyme superoxide dismutase in the cosmopolitan sponge Cliona celata (Grant, 1826), since this enzyme has been described as a useful biomarker for marine pollution in other marine invertebrates. The quantification of the catalytic activity for superoxide dismutase is quite complex because its substrate is an unstable free radical. Several methods have been developed for this enzymatic activity determination; most of them are based on inhibition of a redox reaction involving the superoxide radical. In this work, two methods are compared, for crude sponge extracts, as far as repeatability, reproducibility and sensibility are concerned. The adrenaline oxidation method seems to be the most adequate for these determinations. Statistical treatment of the data indicates that the reference value for the specific superoxide dismutase activity in C. celata should be in the interval [0–535.5] U/mg of total protein in wet tissues, for normal populations.

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Acknowledgments

The authors wish to thank Dr. F.X. Cristobo, for the drawing of the maps and Dr. Gonçalo Calado and Dr. Helena Gaspar for the collection of samples. We also wish to thank all the support from Reserva Natural das Berlengas (Instituto da Conservação da Natureza) and Fundação da Ciência e Tecnologia (POCTI/ QUI/45670/2002) for financial support.

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Authors and Affiliations

  1. Departamento de Química e Bioquímica, Centro de Quimica e Bioquimica, Faculdade de Ciências da Universidade de Lisboa, Edifício C8, Campo Grande, 1749-016, Lisbon, Portugal

    Daniela Marques, Ana I. Esteves, Marise Almeida & Madalena Humanes

  2. Instituto de Tecnologia Biomédica, Faculdade de Medicina Dentária da Universidade de Lisboa, 1649-003, Lisbon, Portugal

    Marise Almeida

  3. Institute for Biodiversity and Ecosystem Dynamics (IBED) and Zoological Museum of Amsterdam (ZMA), University of Amsterdam, Mauritskade 57, 1092 AD, Amsterdam, The Netherlands

    Joana Xavier

  4. CIBIO—Pólo Açores, Centro de Investigação em Biodiversidade e Recursos Genéticos, e Departamento de Biologia, Universidade dos Açores, Rua Mãe de Deus, 9501-855, Ponta Delgada, Portugal

    Joana Xavier

Authors
  1. Daniela Marques
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  2. Ana I. Esteves
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  3. Marise Almeida
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  4. Joana Xavier
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  5. Madalena Humanes
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Corresponding author

Correspondence to Madalena Humanes.

Additional information

Communicated by J.-M. Gili.

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Marques, D., Esteves, A.I., Almeida, M. et al. Superoxide dismutase in the marine sponge Cliona celata . Mar Biol 153, 807–813 (2008). https://doi.org/10.1007/s00227-007-0853-3

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  • DOI: https://doi.org/10.1007/s00227-007-0853-3

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