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Post-settlement energy reserves in Panulirus cygnus: experimental effects of starvation on survival and nutritional condition

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Abstract

Post-settlement survival success in rock (spiny) lobsters is likely to be influenced by the nutritional condition of the non-feeding pueruli at settlement. Consequently, we predict that survival shortly after settlement depends on food resources being replenished rapidly. In the present study the length of the starvation period on survival in post-puerulus juveniles of the western rock lobster Panulirus cygnus was investigated using laboratory experiments. All experimental pueruli were collected from Seven Mile Beach, Western Australia, using sandwich puerulus collectors. Total lipid, lipid class, fatty acid, total protein and total glycogen analyses were conducted on fed and starved post-puerulus juvenile lobsters, from moult into the first juvenile stage to 30 days post-moult. From a total of 165 samples used in this study, 15 post-puerulus juveniles were sampled initially and five post-puerulus juveniles sampled every 5 days thereafter for biochemical analysis. Post-puerulus juveniles survived up to 34 days of starvation. The maximal starvation time for post-puerulus juveniles from which recovery was observed is estimated to be in excess of 22 days. Lipids, in particular phospholipids, are shown to be the most important source of energy catabolised during the starvation period, with greater than 62% reduction in total lipid at day 30. Proteins were also catabolised during starvation, but to a much lesser extent than lipid, decreasing by 34% in total content at day 25. Carbohydrates were present always as a minor constituent, however their content did not change significantly during starvation. Our results suggest that the pueruli collected in December 2004 were generally in sufficiently good condition to survive further starvation after settlement; nutritional levels of the post-puerulus juveniles were enhanced compared to those observed in previous studies. We suggest that the high levels of energy reserves in rock lobster post-pueruli may provide an adaptive advantage to aid the transition from pelagic to benthic living by minimising stress and the consequences of failing to locate immediately suitable food resources.

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References

  • Acosta CA, Butler MJ (1999) Adaptive strategies that reduce predation on Caribbean spiny lobster postlarvae during onshore transport. Limnol Oceanogr 44:494–501

    Article  Google Scholar 

  • Anger K (1989) Growth and exuvial loss during larval and early juvenile development of the hermit crab Pagurus benhardus reared in the laboratory. Mar Biol 103:503–511

    Article  Google Scholar 

  • Anger K (2001) The biology of decapod crustacean larvae. Crustacean issues, 14. AA Balkema, Lisse

  • Anger K, Dawirs RR (1981) Influence of starvation on the larval development of Hyas areneus (Decapoda, Majidae). Helgolander Meeresunters 34:287–311

    Article  Google Scholar 

  • Anger K, Storch V, Anger V, Capuzzo JM (1985) Effects of starvation on moult cycle and hepatopancreas of stage I lobster (Homarus americanus) larvae. Helgolander Meeresunters 39:107–116

    Article  Google Scholar 

  • Barclay MC, Dall W, Smith DM (1983) Changes in lipid and protein during starvation and the moulting cycle in the tiger prawn, Penaeus esculentus Haswell. J Exp Mar Biol Ecol 68:229–244

    Article  CAS  Google Scholar 

  • Benson AA, Lee RF (1975) The role of wax in oceanic food chains. Sci Am 232:77–86

    Article  CAS  Google Scholar 

  • Bligh EG, Dyer WJ (1959) A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37:911–917

    Article  CAS  Google Scholar 

  • Booth JD, Phillips BF (1994) Early life history of spiny lobster. Crustaceana 66:271–294

    Article  Google Scholar 

  • Butler MJ, Herrnkind WF (2000) Puerulus and juvenile ecology. In: Phillips BF, Kittaka J (eds) Spiny lobsters: fisheries and culture. Fishing News Books, Oxford, pp 276–301

    Chapter  Google Scholar 

  • Castell JD (1982) Fatty acid metabolism in crustaceans In: Pruder GD, Langdon CJ, Conklin DE (eds) Proceedings of the second international conference on aquaculture nutrition: biochemical and physiological approaches to shellfish nutrition Louisiana State University Division of Continuing Education, Baton Rouge, pp 124–145

  • Chandumpai A, Dall W, Smith DM (1991) Lipid-class composition of organs and tissues of the tiger prawn Panaeus esculentus during the moulting cycle and during starvation. Mar Biol 108:235–245

    Article  CAS  Google Scholar 

  • Clarke A, Ellis-Evans JC, Sanders MW, Holmes LJ (1989) Patterns of energy storage in Pseudoboeckella poppei (Crustacea, Copepoda) from two contrasting lakes on Signy Island, Antarctica. Hydrobiologia 172:183–191

    Article  Google Scholar 

  • Cockcroft AC (1997) Biochemical composition as a growth predictor in male west-coast rock lobster (Jasus lalandii). Mar Freshw Res 48:845–856

    Article  CAS  Google Scholar 

  • Crisp DJ (1976) The role of the pelagic larva. In: Davies PS (ed) Perspectives in experimental biology and zoology. Pergamon Press, New York, pp 145–155

    Google Scholar 

  • D’Abramo LR (1997) Triacylglycerols and fatty acids. In: D’Abramo LR, Conklin DE, Akiyama DM (eds) Advances in world aquaculture-crustacean nutrition. World Aquaculture Society, Baton Rouge, pp 587

    Google Scholar 

  • Dall W (1974) Indices of nutritional state in the western rock lobster Panulirus longipes (Milne-Edwards). I. Blood tissue constituents and water content. J Exp Mar Biol Ecol 16:167–180

    Article  CAS  Google Scholar 

  • Dall W (1981) Lipid absorption and utilization in the Norwegian lobster, Nephrops norvegicus. J Exp Mar Biol Ecol 50:33–45

    Article  Google Scholar 

  • Dawirs RR (1987) Influence of limited growth and elemental composition (C, H, N) of Carcinus maenas (Decapoda: Portunidae) larvae reared in the laboratory. Mar Biol 93:543–549

    Article  CAS  Google Scholar 

  • de Zwaan A, Zandee DI (1972) Body distribution and seasonal changes in the glycogen content of the common sea mussel Mytilus edulis. Comp Biochem Physiol A 43:53–58

    Article  Google Scholar 

  • Glencross BD, Smith DM (2001) Optimizing the essential fatty acids, eicosapentaenoic and docosahexaenoic acid, in the diet of the prawn, Penaeus monodon. Aquac Nutr 7:101–112

    Article  CAS  Google Scholar 

  • Hagen W, VanVleet ES, Kattner G (1996) Seasonal lipid storage as overwintering strategy of Antarctic krill. Mar Ecol Progress Series 134:85–89

    Article  CAS  Google Scholar 

  • James PJ, Tong LJ (1997) Differences in growth and moult frequency among post-pueruli of Jasus edwardsii fed fresh, aged or frozen mussels. Mar Freshw Res 48:931–934

    Article  Google Scholar 

  • Jeffs AG, Chiswell SM, Booth JD (2001a) Distribution and condition of pueruli of the spiny lobster Jasus edwardsii offshore from north-east New Zealand. Mar Freshw Res 52:1211–1216

    Article  Google Scholar 

  • Jeffs AG, Holland RC (2000) Swimming behaviour of the puerulus of the spiny lobster, Jasus edwardsii (Hutton, 1875) (Decapoda, Palinuridae). Crustaceana 73:847–856

    Article  Google Scholar 

  • Jeffs AG, Nichols PD, Bruce MP (2001b) Lipid reserves used by pueruli of the spiny lobster Jasus edwardsii in crossing the continental shelf of New Zealand. Comp Biochem Physiol A 129:305–311

    Article  CAS  Google Scholar 

  • Jeffs AG, Phleger CF, Nelson MM, Mooney BD, Nichols PD (2002) Marked depletion of polar lipid and non-essential fatty acids following settlement by post-larvae of the spiny lobster Jasus verreauxi. Comp Biochem Physiol A 131:305–311

    Article  Google Scholar 

  • Jeffs AG, Willmott ME, Wells RMG (1999) The use of energy stores in the puerulus of the spiny lobster Jasus edwardsii across the continental shelf of New Zealand. Comp Biochem Physiol A 123:351–357

    Article  Google Scholar 

  • Jernakoff P, Phillips BF, Fitzpatrick JJ (1993) The diet of post-puerulus western rock lobster, Panulirus cygnus George, at Seven Mile Beach, Western Australia. Aust J Mar Freshw Res 44:649–655

    Article  Google Scholar 

  • Jones PL, Obst JH (2000) Effects of starvation and subsequent refeeding on the size and nutrient content of the hepatopancreas of Cherax destructor (Decapoda: Parastacidae). J Crust Biol 20:431–441

    Article  Google Scholar 

  • Kattner G, Wehrtmann IS, Merck T (1994) Interannual variations of lipids and fatty acids during larval development of Crangon spp. in the German Bight, North Sea. Comp Biochem Physiol B 107:103–110

    Article  Google Scholar 

  • Kittaka J (1988) Culture of the palinurid Jasus lalandii from egg stage to puerulus. Nippon Suisan Gakkaishi 54:87–93

    Article  Google Scholar 

  • Koven WM, Kissil GW, Tandler A (1989) Lipid and n-3 requirement of Sparus aurata larvae during starvation and feeding. Aquaculture 79:185–191

    Article  CAS  Google Scholar 

  • Krisman C (1962) A method for the colorimetric estimation of glycogen with iodine. Anal Biochem 4:17–23

    Article  CAS  Google Scholar 

  • Lemmens JWTJ (1994a) Biochemical evidence for absence of feeding in pueruli larvae of the western rock lobster Panulirus cygnus (Decapoda: Palinuridae). Mar Biol 118:383–391

    Article  Google Scholar 

  • Lemmens JWTJ (1994b) The western rock lobster Panulirus cygnus George 1962 (Decapoda: Palinuridae): the effect of temperature and developmental stage on energy requirements of pueruli. J Exp Mar Biol Ecol 180:221–234

    Article  Google Scholar 

  • Lemmens JWTJ (1995) Nucleic acid levels, cellular activity and growth during the puerulus stage of the western rock lobster (Panulirus cygnus George 1962; Decapoda: Palinuridae). J Exp Mar Biol Ecol 194:143–156

    Article  Google Scholar 

  • Liddy GC, Nelson MM, Nichols PD, Phillips BF, Maguire GB (2004) The lipid composition of early stage western rock lobster (Panulirus cygnus) phyllosoma: importance of polar lipid and essential fatty acids. J Shellfish Res 23:265–273

    Google Scholar 

  • Nichols PD, Nichols DS, Bakes MJ (1994) Developments with marine oil products in Australia. AOCS Int News Fats Oils Related Mater 5:254–261

    Google Scholar 

  • Nishida S, Takahashi Y, Kittaka J (1995) Structural changes in the hepatopancreas of the rock lobster, Jasus edwardsii (Crustacea: Palinuridae) during development from the puerulus to post-puerulus. Mar Biol 123:837–844

    Article  Google Scholar 

  • Olsen Y (1999) Lipids and essential fatty acids in aquatic food webs: what can freshwater ecologists learn from mariculture? In: Arts MT, Wainman BC (eds) Lipids in freshwater ecosystems. Springer, New York, pp 161–202

    Chapter  Google Scholar 

  • Pearce A (1997) The nutritional condition of newly settled Jasus edwardsii (Hutton, 1875) (Palinuridae). BSc Hons Thesis, University of Tasmania, Tasmania

  • Phillips BF (1970) A semi-quantitative collector of the puerulus larvae of the western rock lobster Panulirus longipes cygnus George (Decapoda, Palinuridae). Crustaceana 22:147–154

    Article  Google Scholar 

  • Phillips BF, Jeffs AG, Melville-Smith R, Chubb C, Nelson MM, Nichols PD (2006) Changes in lipid and fatty acid composition of late larval and puerulus stages of the spiny lobster (Panulirus cygnus) across the continental shelf of Western Australia. Comp Biochem Physiol B 143:219–228

    Article  Google Scholar 

  • Phillips BF, Melville-Smith R, Rossbach M, Cheng YW, Caputi N, Thomson AW, Mills D, Crear BJ (2003) Towards establishing techniques for large scale harvesting of pueruli and obtaining a better understanding of mortality rates, Fisheries Research Report, FRDC Project 1998/302—Rock Lobster Enhancement & Aquaculture Subprogram, Perth, Australia

  • Phillips BF, Olsen L (1975) Swimming behaviour of the puerulus larvae of the western rock lobster. Aust J Mar Freshw Res 26:415–417

    Article  Google Scholar 

  • Phleger CF, Nelson MM, Mooney BD, Nichols PD, Ritar AJ, Smith GG, Hart PR, Jeffs AG (2001) Lipids and nutrition of the southern rock lobster, Jasus edwardsii, from hatch to puerulus. Mar Freshw Res 52:1475–1486

    Article  CAS  Google Scholar 

  • Querijero BVL, Teshima S, Koshio S, Ishikawa M (1997) Utilization of monounsaturated fatty acid (18:1n-9, oleic acid) by freshwater prawn Macrobrachium rosenbergii (de Man) juveniles. Aquacult Nutr 3:127–139

    Article  CAS  Google Scholar 

  • Ritar AJ, Dunstan GA, Crear BJ, Brown MR (2003) Biochemical composition during growth and starvation of early larval stages of cultured spiny lobster (Jasus edwardsii) phyllosoma. Comp Biochem Physiol A 136:353–370

    Article  Google Scholar 

  • Rosa R, Nunes ML (2003) Seasonal changes in nucleic acids, amino acids and protein content in juvenile Norway lobster (Nephrops norvegicus). Mar Biol 143:565–557

    Article  CAS  Google Scholar 

  • Sasaki GC, Capuzzo JM, Biesiot P (1986) Nutritional and bioenergetic considerations in the development of the American lobster Homarus americanus. Can J Fish Aquat Sci 43:2311–2319

    Article  CAS  Google Scholar 

  • Shieh HS (1969) The biosynthesis of phospholipids in the lobster, Homarus americanus. Comp Biochem Physiol A 30:679–684

    Article  CAS  Google Scholar 

  • Smith GG, Thompson PA, Ritar AJ, Dunstan GA (2003) Effects of starvation and feeding on the fatty acid profiles of stage 1 phyllosoma of the spiny lobster, Jasus edwardsii. Aquacult Res 34:419–426

    Article  CAS  Google Scholar 

  • Speck U, Urich K (1969) Consumption of body constituents during starvation in the crayfish, Orconectes limosus. Z Vgl Physiol 63:410–414

    CAS  Google Scholar 

  • Storch V, Anger K (1983) Influence of starvation and feeding on the hepatopancreas of larval Hyas araneus (Decapoda, Majidae). Helgolander Meeresunters 36:67–75

    Article  Google Scholar 

  • Teshima S, Kanazawa A, Okamoto H (1977) Variation in lipid classes during the molting cycle of the prawn Penaeus japonicus. Mar Biol 39:129–136

    Article  CAS  Google Scholar 

  • Virtue P, Nicol S, Nichols PD (1993) Changes in the digestive gland of Euphausia superba during short-term starvation: lipid class, fatty acid and sterol content and composition. Mar Biol 117:441–448

    CAS  Google Scholar 

  • Volkman JK, Nichols PD (1991) Applications of thin layer chromatography-flame ionization detection to the analysis of lipids and pollutants in marine and environmental samples. J Planar Chromotogr 4:19–26

    CAS  Google Scholar 

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Acknowledgments

We greatly acknowledge the generous financial support provided by the Department of Animal Biology, UWA and CSIRO Marine and Atmospheric Research (Postgraduate Research Scholarship to A.L.). We also acknowledge the invaluable assistance provided by the Western Australian Fisheries Department, which generously provided numerous puerulus with which to conduct all experiments. We would also like to thank Danny Holdsworth, Matt Miller and Ben Mooney for technical support during running of the GC–MS and lipid and fatty acid analyses, and John Carragher and Michael Roberts for providing the glycogen extraction and assay protocol. Thanks to two anonymous referees for helpful comments on the manuscript. Experiments used in this study comply with the current laws of Australia.

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Authors and Affiliations

  1. School of Animal Biology, University of Western Australia, Crawley, Perth, WA, 6009, Australia

    A. J. Limbourn & B. Knott

  2. CSIRO Marine and Atmospheric Research, Wealth from Oceans Flagship, Private Bag No. 5, Wembley, Perth, WA, 6913, Australia

    A. J. Limbourn & R. C. Babcock

  3. Western Australian Fisheries and Marine Research Laboratories, P. O. Box 20, North Beach, Perth, WA, 6920, Australia

    D. J. Johnston

  4. CSIRO Marine and Atmospheric Research, Food Futures Flagship, G. P. O. Box 1538, Hobart, TAS, 7001, Australia

    P. D. Nichols

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  1. A. J. Limbourn
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  2. R. C. Babcock
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  4. P. D. Nichols
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Correspondence to A. J. Limbourn.

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Communicated by S.A. Poulet.

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Limbourn, A.J., Babcock, R.C., Johnston, D.J. et al. Post-settlement energy reserves in Panulirus cygnus: experimental effects of starvation on survival and nutritional condition. Mar Biol 153, 445–456 (2008). https://doi.org/10.1007/s00227-007-0825-7

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