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Photobiology of endolithic microorganisms in living coral skeletons: 1. Pigmentation, spectral reflectance and variable chlorophyll fluorescence analysis of endoliths in the massive corals Cyphastrea serailia, Porites lutea and Goniastrea australensis

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Abstract

We used microscopy, reflectance spectroscopy, pigment analysis, and photosynthesis-irradiance curves measured with variable fluorescence techniques to characterise the endolithic communities of phototrophic microorganisms in the skeleton of three massive corals from a shallow reef flat. Microscopic observations and reflectance spectra showed the presence of up to four distinct bands of photosynthetic microorganisms at different depths within the coral skeleton. Endolithic communities closer to the coral surface exhibited higher photosynthetic electron transport rates and a green zone dominated by Ostreobium quekettii nearest the surface had the greatest chlorophyll pigment concentration. However, Ostreobium was also present and photosynthetically active in the colourless band between the coral tissue and the green band. The spectral properties and pigment density of the endolithic bands were also found to closely correlate to photosynthetic rates as assessed by fluorometry. All endolithic communities were extremely shade-adapted, and photosynthesis was saturated at irradiances <7 μmol photons m−2s−1.

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Abbreviations

ANOVA:

Analysis of variance

CCD:

Charge coupled device

Chl:

Chlorophyll

E b :

Photoinhibition index

E d :

Downwelling irradiance

E k :

Minimum saturating irradiance

E m :

Irradiance at maximum photosynthetic rate

ETR:

Electron transport rate

F m :

Maximum fluorescence in dark

F m′ :

Maximum fluorescence in light

F o :

Minimum fluorescence in dark

F t :

Minimum fluorescence in light

LED:

Light emitting diode

PAR:

Photosynthetically active radiation

P m :

Photosynthetic capacity at saturating irradiance

P s :

Scaling factor

PSII:

Photosystem II

rETRmax :

Relative maximum electron transport rate

RLC:

Rapid light curve

α:

Initial slope of RLC

β:

Slope of RLC after photoinhibition

φPSII :

Effective quantum yield

References

  • Bak RPM, Laane WPM (1987) Annual black bands in skeletons of reef corals (Scleractinia). Mar Ecol Prog Ser 38:169–175

    Article  Google Scholar 

  • Bellamy N, Risk MJ (1982) Coral gas: oxygen production in Millipora on the Great Barrier Reef. Sci NY 215:1618–1619

    Article  CAS  Google Scholar 

  • Delvoye L (1992) Endolithic algae in living stony corals: algal concentrations under influence of depth-dependent light conditions and coral tissue fluorescence in Agarica agaricites (L.) and Meandrina meandrites (L.) (Scleractina, Anthozoa). Stud Nat Hist Caribb Reg 71:24–41

    Google Scholar 

  • Duerden JE (1902) Boring algae as agents in the disintegration of corals. Bull Am Mus Nat Hist 16:323–332

    Google Scholar 

  • Fine M, Loya Y (2001) Endolithic algae: an alternative source of photoassimilates during coral bleaching. Proc R Soc Lond B 269:1205–1210

    Article  Google Scholar 

  • Fine M, Steindler L, Loya Y (2004) Endolithic algae photoacclimate to increased irradiance during coral bleaching. Mar Freshw Res 55:115–121

    Article  CAS  Google Scholar 

  • Fine M, Meroz-Fine E, Hoegh-Guldberg O (2005) Tolerance of endolithic algae to elevated temperature and light in the coral Montipora monasteriata from the southern Great Barrier Reef. J Exp Biol 208:75–81

    Article  Google Scholar 

  • Fork DC, Larkum AWD (1989) Light harvesting in the green alga Ostreobium sp., a coral symbiont adapted to extreme shade. Mar Biol 103:381–385

    Article  Google Scholar 

  • Genty B, Briantais JM, Baker NR (1989) The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence. Biochim Biophys Acta 990:87–92

    Article  CAS  Google Scholar 

  • Grunwald B, Kühl M (2004) A system for imaging variable chlorophyll fluorescence of aquatic phototrophs. Ophelia 58:79–89

    Article  Google Scholar 

  • Halldal P (1968) Photosynthetic capacities and photosynthetic action spectra of endozoic algae of the massive coral Favia. Bull Mar Biol Lab Woods Hole 134:411–424

    Article  CAS  Google Scholar 

  • Harrison WG, Platt T (1986) Photosynthesis–irradiance relationships in polar and temperate phytoplankton populations. Polar Biol 5:153–164

    Article  Google Scholar 

  • Highsmith RC (1981) Lime-boring algae in hermatypic coral skeletons. J Exp Mar Biol 55:267–281

    Article  Google Scholar 

  • Jeffrey SW (1968) Pigment composition of Siphonales algae in the brain coral Favia. Biol Bull 135:141–148

    Article  CAS  Google Scholar 

  • Koehne B, Elli G, Jennings RC, Wilhelm C, Trissl H-W (1999) Spectroscopic and molecular characterization of a long wavelength absorbing antenna of Ostreobium sp. Biochim Biophys Acta 1412:94–107

    Article  CAS  Google Scholar 

  • Kühl M, Jørgensen BB (1992) Spectral light measurements in microbenthic phototrophic communities with a fiber-optic microprobe coupled to a sensitive diode array detector. Limnol Oceanogr 37:1813–1823

    Article  Google Scholar 

  • Kühl M, Cohen Y, Dalsgaard T, Jørgensen BB, Revsbech NP (1995) The microenvironment and photosynthesis of zooxanthellae in scleractinian corals studied with microsensors for O2, pH and light. Mar Ecol Progr Ser 117:159–172

    Article  Google Scholar 

  • Kühl M, Lassen C, Revsbech NP (1997) A simple light meter for measurements of PAR (400–700 nm) with fiber-optic microprobes: application for P versus I measurements in microbenthic communities. Adv Microb Ecol 13:197–207

    Article  Google Scholar 

  • Kühl M, Glud RN, Borum J, Roberts R, Rysgaard S (2001) Photosynthetic performance of surface associated algae below sea ice as measured with a pulse amplitude modulated (PAM) fluorometer and O2 microsensors. Mar Ecol Progr Ser 223:1–14

    Article  Google Scholar 

  • Kühl M (2005) Optical microsensors for analysis of microbial communities. In: Leadbetter JR (ed) Environmental microbiology. Meth Enzym 397:166–199

    Article  Google Scholar 

  • Le Campion-Alsumard T, Goubic S, Hutchings P (1995) Microbial endoliths in skeletons of live and dead corals: Porites lobata (Moorea, French Polynesia). Mar Ecol Progr Ser 117:149–157

    Article  Google Scholar 

  • Lukas KJ (1974) Two species of the chlorophyte genus Ostreobium from skeletons of Atlantic and Caribbean reef corals. J Phycol 10:331–335

    Google Scholar 

  • MacKinney G (1941) Absorption of light by chlorophyll solutions. J Biochem 140:322–355

    Google Scholar 

  • Magnusson SH, Fine M, Kühl M (2006) Light microclimate of endolithic phototrophs in the scleractian corals Montipora monasteriata and Porites cylindrica. Mar Ecol Progr Ser (in press)

  • Odum HT, Odum EP (1955) Trophic structure and productivity of a windward coral reef community on Eniwetok Atoll. Ecol Monogr 25:291–320

    Article  Google Scholar 

  • Platt T, Gallegos CL, Harrison WG (1980) Photoinhibition of photosynthesis in natural assemblages of marine phytoplankton. J Mar Res 38:687–701

    Google Scholar 

  • Priess K, Le Campion-Alsumard T, Golubic S, Gadel F, Thomassin BA (2000) Fungi in corals: black bands and density-banding of Porites lutea and P. lobata skeleton. Mar Biol 136:19–27

    Article  Google Scholar 

  • Ralph PJ, Gademann R, Larkum AWD, Kühl M (2002) Spatial heterogeneity in active fluorescence and PSII activity of coral tissues. Mar Biol 141:639–646

    Article  CAS  Google Scholar 

  • Ralph PJ, Gademann R (2005) Rapid light curves: a powerful tool for the assessment of photosynthetic activity. Aquat Bot 82:222–237

    Article  CAS  Google Scholar 

  • Risk MJ, Müller HR (1983) Porewater in coral heads: evidence for nutrient regeneration. Limnol Oceanogr 28(5):1004–1008

    Article  Google Scholar 

  • Salih A, Larkum A, Cox G, Kühl M., Hoegh-Guldberg O (2000) Fluorescent pigments in coral are photoprotective. Nature 408:850–853

    Article  CAS  Google Scholar 

  • Schlichter D, Kampmann H, Conrady S (1997) Trophic potential and photoecology of endolithic algae living within coral skeletons. PSZN Mar Ecol 18:299–317

    Article  Google Scholar 

  • Schreiber U, Gademann R, Ralph PJ, Larkum AWD (1997) Assessment of photosynthetic performance of Prochloron in Lissoclinum patella by in situ and in hospite chlorophyll fluorescence measurements. Plant Cell Physiol 38:945–951

    Article  CAS  Google Scholar 

  • Schreiber U, Kühl M, Klimant I, Reising H (1996) Measurement of chlorophyll fluorescence within leaves using a modified PAM fluorometer with a fiber-optic microprobe. Photosynth Res 47:103–109

    Article  CAS  Google Scholar 

  • Shashar N, Stambler N (1992) Endolithic algae within corals—life in an extreme environment. J Exp Mar Biol Ecol 163:277–286

    Article  CAS  Google Scholar 

  • Shashar N, Banaszak AT, Lesser MP, Amrami D (1997) Coral endolithic algae: life in a protected environment. Pac Sci 51:167–173

    Google Scholar 

  • Shibata K, Haxo FT (1969) Light transmission and spectral distribution through epi- and endozoic algal layers in the brain coral, Favia. Biol Bull 136:461–468

    Article  CAS  Google Scholar 

  • Sokal RR, Rohlf FJ (1995) Biometry: the principles and practice of statistics in biological research. 3rd edn. WH Freeman and Co. New York

    Google Scholar 

  • Trissl H-W (2003) Modelling the excitation energy capture in thylakoid membranes. In: Larkum AWD, Douglas SE, Raven JA (eds) Photosynthesis in algae. Kluwer, Dordrecht, pp 245–276

    Chapter  Google Scholar 

  • Ulstrup KE, Ralph PJ, Larkum AWD, Kühl M (2006) Intra-colonial variability in light acclimation of zooxanthellae in coral tissues of Pocillopora damicornis. Mar Biol 149:1325–1335

    Article  Google Scholar 

  • Vooren CM (1981) Photosynthetic rates of benthic algae from the deep coral reef of Curacao. Aquat Bot 10:143–154

    Article  Google Scholar 

Download references

Acknowledgments

This study was supported by UTS Internal Funds, the Australian Research Council (PJR and AWDL) and the Danish Natural Science Research Council (MK). A. Glud is thanked for excellent technical assistance. We wish to thank the staff at Heron Island Research station for their support and assistance in this research. All work was carried out under Queensland National Parks and Wildlife Service collection permit G01/623. This is Contribution No. 2 of the Sydney Harbour Institute of Marine Science.

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Correspondence to P. J. Ralph.

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Communicated by G.F. Humphrey.

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Ralph, P.J., Larkum, A.W.D. & Kühl, M. Photobiology of endolithic microorganisms in living coral skeletons: 1. Pigmentation, spectral reflectance and variable chlorophyll fluorescence analysis of endoliths in the massive corals Cyphastrea serailia, Porites lutea and Goniastrea australensis . Mar Biol 152, 395–404 (2007). https://doi.org/10.1007/s00227-007-0694-0

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