Genetic diversity of blackspot seabream (Pagellus bogaraveo) populations off Spanish Coasts: a preliminary study
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Genetic diversity among four natural samples of Blackspot seabream (Pagellus bogaraveo, Brünnich, 1768) from different fishing grounds exploited by Spanish fisheries was analyzed through the use of 12 microsatellite markers. The samples were captured off the Spanish coasts from the Mediterranean Sea to the Cantabrian Sea within the same continental slope. High levels of genetic diversity were revealed for every population and every locus was polymorphic at the 0.95 level. The average number of alleles, average heterozygosity and PIC were found to be 15.75, 0.833 and 0.818, respectively. In general, population differentiation was not detected in these samples. Through AMOVA, a low level of variation between regions (Mediterranean vs. Atlantic samples) was observed, though this was not significant. A larger percentage of total variation was observed inside the ‘within populations’ class. Thus, AMOVA did not reveal any significant population substructure. Moreover, no correlation was found between geographical and FST estimates and the observed results did not allow the improvement of a model of isolation by distance. The high homogeneity revealed by means of these markers could indicate the absence of physical frontiers between the geographical areas analyzed in this survey, especially between Atlantic and Mediterranean areas.
KeywordsMicrosatellite Locus Continental Slope Allelic Richness Atlantic Area Spanish Coast
This study is part of a multidisciplinary project partially funded by JACUMAR. J.A. Piñera’s work is supported by a Ficyt grant from the “Gobierno del Principado de Asturias”.
We would like to thank Yaisel Borrell, Miguel Corujo, Agustín Fernández and David Bernardo for their friendship and their constant help.
- Estoup A, Angers B (1998) Microsatellites and minisatellites for molecular ecology: theoretical and empirical consideration. In: Carvalho GR (ed) Advances in molecular ecology. IOS Press, pp. 55–86Google Scholar
- Excoffier L, Smouse PE, Quattro JM (1995) Analysis of molecular variance inferred from metric distances among DNA haplotypes: application to human mitochondrial DNA restriction data. Genetics 131:479–491Google Scholar
- FAO (2004) The States of world Fisheries and Aquaculture (SOFIA). Available on web: http://www.fao.org/documents/show_cdr.asp?url_file=/DOCREP/007/y5600e/y5600e00.htm
- García de León FJ, Dallas JF, Chatain B, Cannone M, Versini JL, Bonhomme F (1995) Development and use of microsatellite markers in sea bass Dicentrarchus labrax (Linnaeus, 1758) (Perciformes: Serranidae). Mol Mar Biol Biotechnol 4:62–68Google Scholar
- Goudet J (2001) FSTAT, a program to estimate and test gene diversities and fixation indices (version 2.9.3). Available from http://www.unil.ch/izea/softwares/fstat.html
- Hedgecock D (1986) Is gene flow from pelagic larval dispersal important in adaptation and evolution of marine invertebrates? Bull Mar Sci 39:550–564Google Scholar
- Hureau JC, Monod Th (1973) Check-list of the fishes of the north-eastern Atlantic and Mediterranean. Unesco Publication, Paris, pp 426–443Google Scholar
- Orrell TM, Carpenter KE (2004) A phylogeny of the fish family Sparidae (porgies) inferred from mitochondrial sequence data. Mol Phylogenet Evol (in press)Google Scholar
- Schneider S, Roessli D, Excoffier L (2000) Arlequin: software for population genetics data analysis. Ver. 2000. Genetics and Biometry Lab. Dept of Anthropology, University of GenevaGoogle Scholar
- Swofford DL, Selander B (1989) BIOSYS-1: a FORTRAN program for the comprehensive analysis of electrophoretic data in population genetics and systematics. J Hered 72:282–302Google Scholar
- Zardoya R, Castillo R, Grande C, Favre-Krey L, Caetano S, Marcato S, Krey G, Patarnello T (2004) Differential population structuring of two closely related fish species, the mackerel (Scomber scombrus) and the chub mackerel (Scomber japonicus), in the Mediterrenean Sea. Mol Ecol 13:1785–1798CrossRefGoogle Scholar