Abstract
In the present study, the effects of differing salinities on some important functional responses of haemocytes from the clam, Chamelea gallina, were investigated. The animals were kept for 7 days at 28‰ (hyposalinity), 34‰ (control) and 40‰ salinity (hypersalinity), and total haemocyte count (THC), haemocyte volume, phagocytosis, lysozyme-like activity (in both haemocyte lysate and cell-free haemolymph) were measured. The survival-in-air test was also performed. Clams kept at 28‰ showed significantly increased THC with respect to animals kept at 34 and 40‰. The analysis of haemocyte size frequency distribution highlighted that in clams kept at 28‰ the haemocyte fraction of about 5 μm in diameter and 50–100 femtolitre in volume increased markedly. Conversely, in animals kept at 40‰ an increase was observed in the haemocyte fraction having about 8–10 μm diameter and 400–500 femtolitre volume. Higher phagocytic activity was recorded in haemocytes from control clams, with respect to that of clams kept at 28 and 34‰. Lysozyme-like activity in haemocyte lysate was shown to increase significantly in animals kept at 28‰ with respect to that of clams kept at 40‰, whereas enzyme activity in cell-free haemolymph from clams kept at 34‰ was significantly higher with respect to that of clams maintained at 40‰. A relationship between phagocytosis and lysozyme secretion is suggested. The resistance to air exposure of clams kept at 28 and 40‰ was shown to decrease significantly; LT50 values fell from 7 days in clams kept at 34‰ to 4 and 5 days in those kept at 28 and 40‰, respectively. Results demonstrated that salinity values far from 34‰ affects the functional responses of haemocytes and reduce the resistance of clams to exposure to air.
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Babarro JMF, de Zwaan A (2002) Influence of abiotic factors on bacterial proliferation and anoxic survival of the sea mussel Mytilus edulis L. J Exp Mar Biol Ecol 273:33–49
Ballarin L, Cima F, Sabbadin A (1994) Phagocytosis in the colonial ascidian Botryllus schlosseri. Dev Comp Immunol 18:467–481
Bernardi Aubry F, Berton A, Bastianini M, Socal G, Acri F (2004) Phytoplankton succession in a coastal area of the NW Adriatic, over a 10 year sampling period (1990–1999). Cont Shelf Res 24:97–115
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal Biochem 72:248–254
Carballal MJ, Lopez C, Azevedo C, Villalba A (1997) In vitro study of phagocytic ability of Mytilus galloprovincialis Lmk. haemocytes. Fish Shellfish Immunol 7:403–416
Cheng TC (1981) Bivalves. In: Ratcliffe NA, Rowley AF (eds) Invertebrate blood cells 1. Academic, London, pp 233–300
Cheng TC, Rodrick GE, Foley DA, Koehler SA (1975) Release of lysozyme from hemolymph cells of Mercenaria mercenaria during phagocytosis. J Invertebr Pathol 25:261–265
Cheng W, Juang F-M, Chen J-C (2004) The immune response of Taiwan abalone Haliotis diversicolor supertexta and its susceptibility to Vibrio parahaemolyticus at different salinity levels. Fish Shellfish Immunol 16:295–306
Chu F-LE, La Peyre JF (1993) Perkinsus marinus susceptibility and defense-related activities in eastern oysters Crassostrea virginica: temperature effects. Dis Aquat Org 16:223–234
Chu F-LE, La Peyre JF, Burreson C (1993) Perkinsus marinus infection and potential defense-related activities of eastern oysters, Crassostrea virginica: salinity effects. J Invertebr Pathol 62:226–232
de Zwaan A, Eertman RHM (1996) Anoxic or aerial survival of bivalves and other euryoxic invertebrates as a useful response to environmental stress. A comprehensive review. Comp Biochem Physiol 113C:299–312
de Zwaan A, Cortesi P, Cattani O (1995) Resistance of bivalves to anoxia as a response to pollution-induced environmental stress. Sci Total Environ 171:121–125
Eertman RHM, de Zwaan A (1994) Survival of the fittest: resistance of mussels to aerial exposure. In: Kramer KJM (ed) Biomonitoring of coastal waters and estuaries. CRC, Boca Raton, pp 269–284
Eertman RHM, Wagenvoort AJ, Hummel H, Smaal AC (1993) “Survival in air” of the blue mussel Mytilus edulis L. as a sensitive response to pollution-induced environmental stress. J Exp Mar Biol Ecol 170:179–195
Fisher WS, Gauthier JD, Winstead JT (1992) Infection intensity of Perkinsus marinus disease in Crassostrea virginica (Gmelin 1791) from the Gulf of Mexico maintained under different laboratory conditions. J Shellfish Res 11:363–369
Ford SE, Tripp MR (1996) Diseases and defense mechanisms. In: Kennedy VS, Newell RIE, Eble AF (eds) The eastern oyster Crassostrea virginica. Maryland Sea Grant, College Park, pp 581–660
Gagnaire B, Frouin H, Moreau K, Thomas-Guyon H, Renault T (2006) Effects of temperature and salinity on haemocyte activities of the Pacific oyster, Crassostrea gigas (Thunberg). Fish Shellfish Immunol 20:536–547
Gauthier JD, Soniat TM, Rogers JS (1990) A parasitological survey of oysters along salinity gradients in coastal Louisiana. J World Aquac Soc 21:105–115
Gehan EA (1965) A generalized Wilcoxon test for comparing arbitrarily singly censored samples. Biometrika 52:203–223
Goulletquer P, Soletchnik P, Le Moine O, Razet D, Geairon P, Faury N, Taillade S (1998) Summer mortality of the Pacific cupped oyster Crassostrea gigas in the Bay of Marennes-Oleron (France). ICES statutory meeting, Mariculture Committee CM 1998/CC, Copenhagen, 14, pp 21
Haskin HH, Ford SE (1982) Haplosporidium nelsoni (MSX) on Delaware Bay seed oyster beds: a host-parasite relationship along a salinity gradient. J Invertebr Pathol 40:388–405
Hauton C, Hawkins LE, Hutchinson S (1998) The use of the neutral red retention assay to examine the effects of temperature and salinity on haemocytes of the European flat oyster Ostrea edulis (L). Comp Biochem Physiol 119B:619–623
Hauton C, Hawkins LE, Hutchinson S (2000) The effects of salinity on the interaction between a pathogen (Listonella anguillarum) and components of a host (Ostrea edulis) immune system. Comp Biochem Physiol 127B:203–212
Kaplan EL, Meier P (1958) Nonparametric estimation from incomplete observations. J Am Stat Assoc 53:457–481
Lacoste A, Malham SK, Cueff A, Jalabert F, Gélébart F, Poulet SA (2001a) Evidence for a form of adrenergic response to stress in the mollusc Crassostrea gigas. J Exp Biol 204:1247–1255
Lacoste A, Malham SK, Cueff A, Poulet SA (2001b) Stress-induced catecholamine changes in the hemolymph of the oyster Crassostrea gigas. Gen Comp Endocrinol 122:181–188
Lacoste A, Malham SK, Cueff A, Poulet SA (2001c) Noradrenaline modulates oyster hemocyte phagocytosis via a β-adrenergic receptor-cAMP signaling pathway. Gen Comp Endocrinol 122:252–259
Lopez C, Carballal MJ, Azevedo C, Villalba A (1997) Enzyme characterisation of the circulating haemocytes of the carpet shell clam, Ruditapes decussatus (Mollusca: Bivalvia). Fish Shellfish Immunol 7:595–608
Matozzo V, Monari M, Foschi J, Papi T, Cattani O, Marin MG (2005). Exposure to anoxia of the clam Chamelea gallina. I: effects on immune responses. J Exp Mar Biol Ecol 325:163–174
Mohandas A, Cheng TC, Cheng JB (1985) Mechanism of lysosomal enzyme release from Mercenaria mercenaria granulocytes: a scanning electron microscope study. J Invertebr Pathol 45:189–197
Monari M, Matozzo V, Foschi J, Marin MG, Cattani O (2005) Exposure to anoxia of the clam, Chamelea gallina. II: modulation of superoxide dismutase activity and expression in haemocytes. J Exp Mar Biol Ecol 325:175–188
Monari M, Matozzo V, Foschi J, Cattani O, Serrazanetti GP, Marin MG (2007) Effects of high temperatures on functional responses of haemocytes in the clam Chamelea gallina. Fish Shellfish Immunol 22:98–114
Navarro JM, Gonzalez CM (1998) Physiological responses of the Chilean scallop Argopecten purpuratus to decreasing salinities. Aquaculture 167:315–327
Oliver LM, Fisher WS (1999) Appraisal of prospective bivalve immunomarkers. Biomarkers 4:510–530
Pipe RK, Coles JA (1995) Environmental contaminants influencing immune function in marine bivalve molluscs. Fish Shellfish Immunol 5:581–595
Reid HI, Soudant P, Lambert C, Paillard C, Birkbeck TH (2003) Salinity effects on immune parameters of Ruditapes philippinarum challenged with Vibrio tapetis. Dis Aquat Org 56:249–258
Shumway SE (1996) Natural environmental factors. In: Kennedy VS, Newell RIE, Eble AF (eds) The eastern oyster Crassostrea virginica. Maryland Sea Grant, College Park, pp 467–513
Wang L-U, Chen J-C (2005) The immune response of white shrimp Litopenaeus vannamei and its susceptibility to Vibrio alginolyticus at different salinity levels. Fish Shellfish Immunol 18:269–278
Wang F-I, Chen J-C (2006) Effect of salinity on the immune response of tiger shrimp Penaeus monodon and its susceptibility to Photobacterium damselae subsp. damselae. Fish Shellfish Immunol 20:671–681
Acknowledgments
This work was supported by grants from the Italian MiPAF to Dr. O. Cattani (contr. n° 6C66). The authors wish to thank Keith Smith for revising the English text.
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Communicated by R. Cattaneo-Vietti, Genova.
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Matozzo, V., Monari, M., Foschi, J. et al. Effects of salinity on the clam Chamelea gallina. Part I: alterations in immune responses. Mar Biol 151, 1051–1058 (2007). https://doi.org/10.1007/s00227-006-0543-6
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DOI: https://doi.org/10.1007/s00227-006-0543-6