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Time-course uptake and elimination of benzo(a)pyrene and its damage to reproduction and ensuing reproductive outputs of Pacific oyster, Crassostrea gigas

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Abstract

The time-course of uptake and elimination of benzo(a)pyrene (BaP) for the Pacific oyster, Crassostrea gigas and reproduction damage and reproductive outputs were studied. Sexually immature C. gigas broodstock were fed for 28 days with live algae grown in four BaP solutions of 0, 50, 500, and 5,000 μg L−1 (hereafter, control, 50, 500, and 5,000 oysters) and were subsequently conditioned to maturation by a feeding with BaP-free live algae under temperature manipulation for another 28 days. The 5,000 μg L−1 oysters gained a steady state concentration, around 30,000 ng g−1 d.w. for digestive gland, a week earlier compared to the 500 μg L−1 oysters. The earlier gain or longer persistence of the steady state concentration influenced elimination of BaP, with an eliminating trend for 500 μg L−1 oysters, while no elimination for 5,000 μg L−1 oysters. The maternal persistence of the steady state concentration resulted in significant damages in the reproductive success and their reproductive outputs in terms of the hatching rate and larval growth, survival, and settlement. The 50 μg L−1 oysters remained far below the steady state concentration, and showed a manifest eliminating behavior during the subsequent BaP-free 28 day maturation period. The reproductive success and initial larval events of 50 μg L−1 oysters were comparable to those of control. However, the damage potential of the 50 μg L−1 oysters might be more significant if their maternal exposure continued beyond 28 days, since the accumulation profile at this dose was linear.

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Acknowledgments

The research was funded by National Fisheries Research and Development Institute, Ministry of Maritime Affairs and Fisheries, South Korea.

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Authors and Affiliations

  1. Department of Biology, Pusan National University, Busan, 609-735, South Korea

    Eun Jung Choy & Chang-Keun Kang

  2. National Fisheries Research and Development Institute, Busan, 619-902, South Korea

    Qtae Jo & Hyo-Bang Moon

  3. Department of Aquatic Life Medicine, Pukyong National University, Busan, 608-737, South Korea

    Ju-Chan Kang

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  1. Eun Jung Choy
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Correspondence to Qtae Jo.

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Communicated by S. Nishida, Tokyo.

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Choy, E.J., Jo, Q., Moon, HB. et al. Time-course uptake and elimination of benzo(a)pyrene and its damage to reproduction and ensuing reproductive outputs of Pacific oyster, Crassostrea gigas . Mar Biol 151, 157–165 (2007). https://doi.org/10.1007/s00227-006-0464-4

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