Marine Biology

, Volume 150, Issue 6, pp 1227–1236 | Cite as

Population differences in nerve resistance to paralytic shellfish toxins in softshell clam, Mya arenaria, associated with sodium channel mutations

  • Laurie B. Connell
  • Scott P. MacQuarrie
  • Betty M. Twarog
  • Melissa Iszard
  • V. Monica Bricelj
Research Article

Abstract

The softshell clam, Mya arenaria, is a commercially important bivalve with wide latitudinal distribution in North America. Populations of clams with a history of repeated exposure to toxic Alexandrium spp. have developed a natural resistance to the paralytic shellfish toxins (PSTs) produced by these algae. An association between PST resistance in individual clams and a single mutation in the saxitoxin (STX) binding region of the α-subunit of the voltage-gated sodium (Na+) channel gene was previously identified. Here we establish that more than one mutation associated with nerve resistance to STX occurred at this locus. Both cDNA from mRNA and genomic DNA sequences from individual clams are identical demonstrating that both alleles are expressed simultaneously. In addition, one resistant allele per individual is sufficient to confer neural resistance to STX even though heterozygous individuals show an intermediate level of resistance to STX in in vitro nerve trunk assays.

Notes

Acknowledgments

We thank the many individuals that assisted in field sample collection: B. Myrand (MAPAQ, Québec), D. Whitaker, J. Kennedy, L. Savina, S. Cunningham, D. Farbes (Massachusetts DMF), G. Gillespie (DFO BC), N. Adams and B. Bill (NOAA NWFSC). We also thank anonymous reviewers for detailed comments that have led to improvement of the final version of this manuscript. This work was supported by a NOAA-ECOHAB #NA 17OP1451 grant to LBC, VMB and V. Trainer. This is IMB/NRC publication #42562.

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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Laurie B. Connell
    • 1
  • Scott P. MacQuarrie
    • 2
  • Betty M. Twarog
    • 3
  • Melissa Iszard
    • 1
  • V. Monica Bricelj
    • 2
  1. 1.School of Marine Sciences University of MaineOronoUSA
  2. 2.Institute for Marine Biosciences National Research CouncilHalifaxCanada
  3. 3.Darling Marine CenterUniversity of MaineWalpoleUSA

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