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Rearing Pandalus borealis larvae in the laboratory

II. Routine oxygen consumption, maximum oxygen consumption and metabolic scope at three temperatures

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Abstract

Larvae of the northern shrimp Pandalus borealis (Krøyer) are pelagic. In the Estuary and Gulf of St. Lawrence, Canada, the early stages are found in the upper 25-m of the water column in spring and early summer and are expected to experience a range of water temperatures from as low as 0°C to as high at 6°C. Little is known of the impact of water temperature on metabolic requirements of northern shrimp larvae. In this study, routine respiration (VO2), maximum respiration (electron transport system activity, ETSA) and metabolic scope for growth (MS, ETSA–VO2) of northern shrimp larvae were measured as a function of temperature (3, 5 and 8°C), developmental stage (I–V at 3°C, I–VII at 5°C and 8°C) and growth rate in dry mass. After logarithmic transformation, all three metabolic variables were linearly related to dry mass. The increase in VO2 with body mass was faster at 5°C than at 3 or 8°C, whereas with ETSA this increase was slower. As a result, MS increased more slowly with dry mass at 5°C than at 3 and 8°C. However, MS did not limit growth in this study, since it explained only 39% of the variability in growth. All three metabolic variables as well as growth varied together as a function of temperature and ontogeny. Q10 of all three metabolic variables ranged from 1.6 and 2.2 for stages I–V larvae, except for VO2 at stage I (3.9) and stage III (2.9).

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Acknowledgements

We are very grateful to Jean-Pierre Allard for his able handling of the rearing of P. borealis larvae, to Roberta Miller and Véronique Allard for performing the measurements of routine respiration, and to Nathalie Côté for the determinations of ETSA and total protein contents. We also thank Roberta Miller for reviewing the manuscript. These experiments followed the recommendations of the Canadian Council for the Protection of Animals.

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Authors and Affiliations

  1. Fisheries and Oceans Canada, Maurice Lamontagne Institute, 850 route de la Mer, Mont-Joli, Quebec , G5H 3Z4, Canada

    Denis Chabot & Patrick Ouellet

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  1. Denis Chabot
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  2. Patrick Ouellet
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Correspondence to Denis Chabot.

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Communicated by R.J. Thompson, St. John’s

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Chabot, D., Ouellet, P. Rearing Pandalus borealis larvae in the laboratory. Marine Biology 147, 881–894 (2005). https://doi.org/10.1007/s00227-005-1626-5

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