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Genetic divergence between two morphologically similar varieties of the kuruma shrimp Penaeus japonicus

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Abstract

The kuruma shrimp Penaeus japonicus is widely distributed throughout the Indo-West Pacific. Two morphologically similar varieties, I and II, are recognized from the South China Sea. The two varieties are characterized by different color banding patterns on the carapace, but there are no distinct differences in morphometric traits between them based on measurement of 13 characters. Sequence data and restriction profiles of the mitochondrial genes reveal that these two varieties represent distinct clades, with sequence divergences of about 1% (473 bp) in 16S rRNA, 6–7% (504 bp) in cytochrome oxidase I, and 16–19% (470 bp) in the control region. Analysis of amplified fragment length polymorphism confirms that the two varieties are genetically distinct. We also investigated the geographical distribution of the two varieties in the western Pacific by analyzing specimens collected from Japan and Singapore. Shrimps from Japan and Singapore have been found to belong to varieties I and II, respectively, suggesting that the two varieties have different geographical distribution. Phylogenetic study reveals that the two varieties are more closely related to each other than to the other phylogenetically related Penaeus species. Results from this study suggest the occurrence of two cryptic species in the kuruma shrimp P. japonicus.

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Acknowledgements

Sincere thanks are extended to P.K.L. Ng (National University of Singapore), K. Tanaka (Kyorin University, Japan), Y.H. Yung (The Chinese University of Hong Kong), J. Wong and P.H. Wong (Princess Margaret Hospital, Hong Kong) for collecting specimens. We are indebted to T.Y. Chan (National Taiwan Ocean University) for examination of the specimens and discussion on this project. We also thank J. Tong (Institute of Hydrobiology, Chinese Academy of Sciences), Y.K. Tam, C.P. Li, and W. Lau (The Chinese University of Hong Kong) for technical assistance, and C.K. Wong (The Chinese University of Hong Kong) and an anonymous reviewer for constructive comments on the manuscript. The work described in this article was fully supported by a grant from the Research Grants Council, Hong Kong Special Administrative Region (HKSAR), China (Project no. CUHK4157/01 M). The experiments complied with the current laws of HKSAR.

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Authors and Affiliations

  1. Department of Biology, The Chinese University of Hong Kong, Shatin, Hong Kong, China

    K. H. Tsoi, Z. Y. Wang & K. H. Chu

  2. Fisheries College, Jimei University, 361021, Xiamen, Fujian, China

    Z. Y. Wang

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  1. K. H. Tsoi
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  2. Z. Y. Wang
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Correspondence to K. H. Chu.

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Communicated by M.S. Johnson, Crawley

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Tsoi, K.H., Wang, Z.Y. & Chu, K.H. Genetic divergence between two morphologically similar varieties of the kuruma shrimp Penaeus japonicus. Marine Biology 147, 367–379 (2005). https://doi.org/10.1007/s00227-005-1585-x

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