Marine Biology

, Volume 149, Issue 2, pp 213–226 | Cite as

Genetic structure and phylogeography of the lined shore crab, Pachygrapsus crassipes, along the northeastern and western Pacific coasts

  • Bryan J. CassoneEmail author
  • Elizabeth G. Boulding
Research Article


Marine invertebrates with high larval dispersal capacity typically exhibit low degrees of population differentiation, which reflects both contemporary and historical processes. We sampled 346 individuals from seven populations of the lined shore crab, Pachygrapsus crassipes Randall, along the northeastern Pacific Coast and Korea during summer 2003. DNA sequence analysis of 613 bp of the mitochondrial COI gene showed that overall gene diversity (h) was high (0.92±0.01), whereas overall nucleotide diversity (π) was low (0.009±0.005). A total of 154 mtDNA haplotypes were identified; however, 114 were present in only one individual. Analysis of molecular variance revealed significant genetic structuring at Point Conception, CA, USA, that is likely due to the oceanographic circulation patterns, which result in asymmetrical migration of haplotypes. However, genetic variation among eastern Pacific populations was generally low, probably because of high contemporary gene flow and recent common ancestry of haplotypes. Mismatch analysis and nested clade analysis suggested that the population history of this region is characterized by two contiguous northwards range expansions, which are congruent with Late Pleistocene glacial cycles. Highly significant genetic differentiation was detected between eastern Pacific populations and Korea, indicating transpacific gene flow is restricted. Time of divergence between the two transpacific lineages was estimated between 0.8 and 1.2 Myrs ago. The small, recently founded population of P. crassipes at Bamfield, BC, Canada, did not appear to have undergone a founder effect.


Mismatch Distribution Point Conception Nest Clade Analysis Sudden Population Expansion Effective Female Population Size 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



We wish to thank C. Schubart for his P. transversus samples, as well as T. Crease, T. Zahradnik, R. McCarthy, and J. Ackerman for their valuable insight on various aspects of this study. We also greatly thank H.-J. Lee for collecting samples from Korea, and the directors and staff of Bamfield Marine Station, Oregon Institute of Marine Biology, Bodega Bay Marine Lab, and CICESE for field assistance. This work was supported by NSERC Discovery and PREA grants to E.G. Boulding. All experiments complied with the current laws of Canada.


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Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  1. 1.Department of Biological SciencesUniversity of Notre DameNotre DameUSA
  2. 2.Department of Integrative BiologyUniversity of GuelphGuelphCanada

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