Marine Biology

, Volume 148, Issue 1, pp 35–41 | Cite as

Transmission, plasticity and the molecular identification of cyanobacterial symbionts in the Red Sea sponge Diacarnus erythraenus

  • Matan Oren
  • Laura SteindlerEmail author
  • Micha Ilan
Research Article


Cyanobacterial symbionts in the sponge Diacarnus erythraenus from the Red Sea were identified in both adult sponges and their larvae by 16S rDNA sequencing. A single cyanobacterial type was found in all samples. This cyanobacterial type is closely related to other sponge cyanobacterial symbionts. The cyanobacterial rDNA, together with the morphological analysis by electron and fluorescence microscopy, provided evidence for vertical transmission of the symbionts in this sponge. In addition, we show phenotypic plasticity of the symbionts inside the sponge, probably as a result of variability in light availability inside the sponge tissue. Finally, the reproduction of Diacarnus erythraenus is also described.


Sponge Synechococcus Vertical Transmission Marine Sponge Sponge Species 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This work was financially supported by Grant 2000-321 from the United States–Israel Binational Science Foundation (BSF) to M. Ilan, S. Beer and J.R. Pawlik. I. Brickner is thanked for histological preparations, and Y. Delarea for assistance with electron microscopy. S. Beer is thanked for his editorial comments of the manuscript. We thank the staff of the Inter-University Institute in Elat for their help during the course of this study. Samples were collected in compliance with the 2003/16729 permit from the Israel Nature and National Parks Protection Authority.


  1. Bernatzky R, Tanksley SD (1986) Methods for detection of single or low copy sequences in tomato on Southern blots. Plant Mol Biol Rep 4:37–41CrossRefGoogle Scholar
  2. Diaz M, Ward B (1997) Molecular detection and characterization of specific bacterial groups associated to tropical sponges. Proc 8th Internat Coral Reef Symp 2:1399–1402Google Scholar
  3. Gaino E, Burlando B, Buffa P, Sarà M (1987) Ultrastructural study of the mature egg of Tethya citrina Sara and Melone (Polifera, Demospongiae). Gamete Res 16:259–265CrossRefGoogle Scholar
  4. Herre E, Knowlton N, Mueller U (1999) The evolution of mutualisms: exploring the paths between conflict and cooperation. Trends Ecol Evol 14:49–53CrossRefGoogle Scholar
  5. Honda D, Yokota A, Sugiyama J (1999) Detection of seven major evolutionary lineages in cyanobacteria based on the 16S rRNA gene sequence analysis with new sequences of five marine Synechococcus strains. J Mol Evol 48:723–739CrossRefGoogle Scholar
  6. Levi C, Levi P (1976) Embryogenese de Chondrosia reniformis (NARDO) demosponge ovipare, et transmission des bacteries symbiotiques. Ann Sci Nat 18:367–380Google Scholar
  7. Regoli F, Cerrano C, Chierici E, Bompadre S, Bavestrello G (2000) Susceptibility to oxidative stress of the Mediterranean demosponge Petrosia ficiformis: role of endosymbionts and solar irradiance. Mar Biol 137:453–461CrossRefGoogle Scholar
  8. Robertson BR, Tezuka N, Watanabe MM (2001) Phylogenetic analyses of Synechococcus strains (cyanobacteria) using sequences of 16S rDNA and part of the phycocyanin operon reveal multiple evolutionary lines and reflect phycobilin content. Int J Syst Evol Microbiol 51:861–871CrossRefGoogle Scholar
  9. Sanger F (1981) Determination of nucleotide sequence in DNA. Science 214:1205–1210CrossRefGoogle Scholar
  10. Sarà M, Liaci L (1964) Associazione fra la Cianoficea Aphanocapsa feldmanni e alcune Demospongiae. Marine Boll Zool 31:5–65CrossRefGoogle Scholar
  11. Sarà M, Bavestrello G, Cattaneo-Vietti R, Cerrano C (1998) Endosymbiosis in sponges: Relevance for epigenesis and evolution. Symbiosis 25:57–70Google Scholar
  12. Scalera Liaci L, Sciscioli M, Matarrese A (1971) La reproduzione sessuale di alcuni Tetractinomorpha (Porifera). Atti. Soc. Peloritana Scienze Fis Matem Natur 17:235–245Google Scholar
  13. Scalera Liaci L, Sciscioli M, Materrese A (1973) Raffronto tra il comportamento sessuale di alcune Ceractinomorpha. Riv Biol 66:135–153PubMedGoogle Scholar
  14. Sciscioli M, Lepore E, Gherardi M, Scalera Liaci L (1994) Transfer of symbiotic bacteria in the mature oocyte of Geodia cydonium (Porifera, Demospongiae): an ultrastructural study. Cah Biol Mar 35:471–478Google Scholar
  15. Steindler L, Huchon D, Avni A, Ilan M (2005) 16S rRNA phylogeny of sponge-associated cyanobacteria. Appl Environ Microbiol 71:4127–4131CrossRefGoogle Scholar
  16. Thacker RW, Starnes S (2003) Host specificity of the symbiotic cyanobacterium Oscillatoria spongeliae in marine sponges, Dysidea spp. Mar Biol 142:643–648CrossRefGoogle Scholar
  17. Unson MD, Faulkner DJ (1993) Cyanobacterial symbiont biosynthesis of chlorinated metabolites from Dysidea herbacea (Porifera). Experientia 49:349–353CrossRefGoogle Scholar
  18. Usher KM, Kou J, Fromont J, Sutton DC (2001) Vertical transmission of cyanobacterial symbionts in the marine sponge Chondrilla australiensis (Demospongiae). Hydrobiologia 461:15–23CrossRefGoogle Scholar
  19. Usher KM, Fromont J, Sutton DC, Toze S (2004a) The biogeography and phylogeny of unicellular cyanobacterial symbionts in sponges from Australia and the Mediterranean. Microbial Ecol 48:167–177CrossRefGoogle Scholar
  20. Usher KM, Sutton DC, Toze S, Kou J, Fromont J (2005) Inter-generational transmission of microbial symbionts in the marine sponge Chondrilla australiensis (Demospongiae). Mar Fresh Res 56:125–131CrossRefGoogle Scholar
  21. Usher KM, Toze S, Fromont J, Kuo J, Sutton DC (2004b) A new species of cyanobacterial symbiont from the marine sponge Chondrilla nucula. Symbiosis 36:183–192Google Scholar
  22. Wilkinson CR (1979) Nutrient translocation from symbiotic cyanobacteria to coral reef sponges. In: Levi C, Boury-Esnault N (eds) Colloques internationaux du CNRS No 291 - Biologie des spongiaires. Centre National de la Recherche Scientifique, Paris, pp 373–380Google Scholar
  23. Wilkinson CR (1981) Significance of sponge with cyanobacterial symbionts on Davies Reef, Great Barrier Reef Proc 4th internat. Coral Reef Symp Manila 2:705–712Google Scholar
  24. Wilkinson CR (1983) Phylogeny of bacterial and cyanobacterial symbionts in marine sponges. Endocytobiology 2:993–1002Google Scholar
  25. Wilkinson CR, Fay P (1979) Nitrogen fixation in coral reef sponges with symbiotic cyanobacteria. Nature 279:527–529CrossRefGoogle Scholar
  26. Yamamura N (1993) Vertical transmission and evolution of mutualism from parasitism. Popul Biol 44:95–109CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  1. 1.Department of Zoology, George S. Wise Faculty of Life SciencesTel Aviv UniversityTel AvivIsrael
  2. 2.Department of Plant Sciences, George S. Wise Faculty of Life SciencesTel Aviv UniversityTel AvivIsrael

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