Marine Biology

, Volume 148, Issue 1, pp 25–34 | Cite as

Measurement of chlorophyll fluorescence reveals mechanisms for habitat niche separation of the intertidal seagrasses Thalassia hemprichii and Halodule uninervis

  • Chiu-Yueh Lan
  • Wen-Yuan Kao
  • Hsing-Juh LinEmail author
  • Kwang-Tsao Shao
Research Article


In Taiwan, Thalassia hemprichii dominates the upper intertidal zone, whereas Haloduleuninervis occupies the lower intertidal zone. We tested the hypothesis that T. hemprichii is better adapted to high irradiance and more resistant to air exposure than H. uninervis. The photosynthetic efficiency, damage, and extent of recovery were determined by measuring chlorophyll fluorescence using pulse amplitude modulated fluorometry. Both species growing in tidal pools, in response to high irradiance alone, revealed a small depression in maximal quantum yield of photosystem II (Fv/Fm) at noon. The second experiment compared the effect of air exposure alone and the combined effect of air exposure with high irradiance by interposing a shading screen on both species, growing in the intertidal zone over a diurnal cycle. Values of Fv/Fm of both the shaded and irradiated T. hemprichii remained high at low tide. However, H. uninervis exhibited a marked depression following air exposure and a synergistic depression under the combined effect. The experimental manipulations of exposure time demonstrated that the tolerance of T. hemprichii to the combined effect was longer and the recovery from air exposure following re-submersion was better than those of H. uninervis. Both species were more susceptible to the combined effect in the dry season than in the wet season. Our results suggest that air exposure is more important than high irradiance in constraining the distribution of H. uninervis in the upper intertidal zone. This was confirmed by transplantation experiments in which a rapid decline of H. uninervis was observed after transplantation into the upper intertidal zone. In the lower intertidal zone, measurements of the response of the photosynthetic electron transport rate to irradiance demonstrated that the transplanted T. hemprichii exhibited a sun-type response and H. uninervis a shade-type response.


Chlorophyll Fluorescence Diurnal Cycle Intertidal Zone Photosynthetic Efficiency High Irradiance 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This study was supported by the National Science Council under grant number NSC90-2621-B-005-008 and Kenting National Park under grant number 092-301020100G-004. This manuscript was improved by the comments of Prof. S. Beer and one anonymous reviewer.


  1. Adams JB, Bate GC (1994) The tolerance to desiccation of the submerged macrophytes Ruppia cirrhosa (Petagna) Grande and Zostera capensis Setchell. J Exp Mar Biol Ecol 183:53–62CrossRefGoogle Scholar
  2. Beer S, Björk M (2000) Measuring rates of photosynthesis of two tropical seagrasses by pulse amplitude modulated (PAM) fluorometry. Aquat Bot 66:69–76CrossRefGoogle Scholar
  3. Beer S, Vilenkin B, Weil A, Veste M, Susel L, Eshel A (1998) Measuring photosynthetic rates in seagrasses by pulse amplitude modulated (PAM) fluorometry. Mar Ecol Prog Ser 174:293–300CrossRefGoogle Scholar
  4. Björk M, Weil A, Semesi S, Beer S (1997) Photosynthetic utilization of inorganic carbon by seagrasses from Zanzibar, East Africa. Mar Biol 129: 363–366CrossRefGoogle Scholar
  5. Björk M, Uku J, Weil A, Beer S (1999) Photosynthetic tolerances to desiccation of tropical intertidal seagrasses. Mar Ecol Prog Ser 191:121–126CrossRefGoogle Scholar
  6. Buchanan JB, Kain JM (1971) Measurement of the physical and chemical environment. In: Holme NA, McIntyre AD (eds.), Methods for the Study of Marine Benthos. IBP Handbook no. 16. Blackwell Scientific Publications, Oxford, UK, pp 30–58Google Scholar
  7. Dawes CJ (1998) Marine Botany, 2nd edn. Wiley, New YorkGoogle Scholar
  8. Dawson SP, Dennison WC (1996) Effects of ultraviolet and photosynthetically active radiation on five seagrass species. Mar Biol 125:629–638CrossRefGoogle Scholar
  9. Fourqurean JW, Zieman JC, Powell GVN (1992) Relationships between porewater nutrients and seagrasses in a subtropical carbonate environment. Mar Biol 114:57–65Google Scholar
  10. Fourqurean JW, Powell GVN, Kenworthy WJ, Zieman JC (1995) The effects of long-term manipulation of nutrient supply on competition between the seagrasses Thalassia testudinum and Halodule wrightii in Florida Bay. Oikos 72:349–358CrossRefGoogle Scholar
  11. Gallegos ME, Merino M, Rodriquez A, Marbà N, Duarte CM (1994) Growth patterns and demography of pioneer Caribbean seagrasses Halodule wrightii and Syringodium filiforme. Mar Ecol Prog Ser 109:99–104CrossRefGoogle Scholar
  12. Hsieh HL (1995) Spatial and temporal patterns of polychaete communities in a subtropical mangrove swamp: influences of sediment and microhabitat. Mar Ecol Prog Ser 127:157–167CrossRefGoogle Scholar
  13. Iverson RL, Bittaker HF (1986) Seagrass distribution and abundance in eastern Gulf of Mexico coastal waters. Estuar Coast Shelf Sci 22:577–602CrossRefGoogle Scholar
  14. Jassby AD, Platt T (1976) Mathematical formulation of the relationship between photosynthesis and light for phytoplankton. Limnol Oceanogr 21:540–547CrossRefGoogle Scholar
  15. Jupp BP, Durako MJ, Thayer GW, Schilak L (1996) Distribution, abundance, and species composition of seagrasses at several sites in Oman. Aquat Bot 53:199–213CrossRefGoogle Scholar
  16. Krause GH, Weis E (1991) Chlorophyll fluorescence and photosynthesis: the basics. Annu Rev Plant Physiol Plant Mol Biol 42:313–349CrossRefGoogle Scholar
  17. Leuschner C, Landwehr S, Mehlig U (1998) Limitation of carbon assimilation of intertidal Zostera noltii and Z marina by desiccation at low tide. Aquat Bot 62:171–176CrossRefGoogle Scholar
  18. Lin HJ, Shao KT (1998) Temporal changes in the abundance and growth of intertidal Thalassia hemprichii seagrass beds in southern Taiwan. Bot Bull Acad Sin 39:191–198Google Scholar
  19. Mukai H (1993) Biogeography of the tropical seagrasses in the western Pacific. Aust J Mar Freshw Res 44:1–17Google Scholar
  20. Pai SC, Yang CC, Riley JP (1990) Formation kinetics of the pink azo dye in the determination of nitrite in natural waters. Anal Chem Acta 232:345–349CrossRefGoogle Scholar
  21. Powell GVN, Fourqurean JW, Kenworthy WJ, Zieman JC (1991) Bird colonies cause seagrass enrichment in a subtropical estuary: observational and experimental evidence. Estuar Coast Shelf Sci 32:567–579CrossRefGoogle Scholar
  22. Rose CD, Dawes CJ (1999) Effects of community structure on the seagrass Thalassia testudinum. Mar Ecol Prog Ser 184:83–95CrossRefGoogle Scholar
  23. Seddon S, Cheshire AC (2001) Photosynthetic response of Amphibolis antarctica and Posidonia australis to temperature and desiccation using chlorophyll fluorescence. Mar Ecol Prog Ser 220:119–130CrossRefGoogle Scholar
  24. Silva J, Santos R (2003) Daily variation patterns in seagrass photosynthesis along a vertical gradient. Mar Ecol Prog Ser 257:37–44CrossRefGoogle Scholar
  25. Sokal RR, Rohlf FJ (1995) Biometry, 3rd edn. WH Freeman, New YorkGoogle Scholar
  26. Stapel J, Munumtun R, Hemminga MA (1997) Biomass loss and nutrient redistribution in and Indonesian Thalassia hemprichii seagrass bed following seasonal low tide exposure during day light. Mar Ecol Prog Ser 148:251–262CrossRefGoogle Scholar
  27. Strickland JD, Parsons TR (1972) A practical handbook of seawater analysis, 2nd edn. Fisheries Research Board Canada, OttawaGoogle Scholar
  28. Tilman D (1985) The resource-ratio hypothesis of plant succession. Am Nat 125:827–852CrossRefGoogle Scholar
  29. Whittaker RH, Feeney PP (1971) Allelochemics: chemical interactions between species. Science 171:757–770CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Chiu-Yueh Lan
    • 1
  • Wen-Yuan Kao
    • 2
  • Hsing-Juh Lin
    • 3
    Email author
  • Kwang-Tsao Shao
    • 4
  1. 1.Department of Life SciencesNational Chung Hsing UniversityTaiwanRepublic of China
  2. 2.Department of Life ScienceNational Taiwan UniversityTaiwanRepublic of China
  3. 3.Department of Life SciencesNational Chung Hsing UniversityTaiwanRepublic of China
  4. 4.Research Center for BiodiversityAcademia SinicaTaiwanRepublic of China

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