Advertisement

Marine Biology

, Volume 147, Issue 6, pp 1367–1375 | Cite as

Comparison of five methods for estimating growth of Calanus helgolandicus later developmental stages (CV–CVI)

  • L. YebraEmail author
  • R. P. Harris
  • T. Smith
Research Article

Abstract

The activity of the enzymes aminoacyl-tRNA synthetases (AARS) in adult females and males and copepodites stage V of Calanus helgolandicus was studied at the L4 time-series station in the English Channel from June 2002 to December 2003. AARS activity was explored as an index of somatic growth in the laboratory as well as in the field by comparison with other methods of measuring growth: (1) the direct method (Heinle in Chesapeake Sci 7:59–74, 1966), (2) the weight increment (WI) method, (3) the Hirst and Bunker (HB) equation (Limnol Oceanogr 48(5):1988–2010, 2003) and (4) the egg production (EPR) method. AARS activity showed a significant correlation with the direct measurement of growth in the laboratory (R 2=0.55). However, the correlation was lower for growth assessed either with the WI or the HB approaches in the field (R 2=0.05–0.17). Female AARS activity showed a positive correlation with specific EPR during the reproductive season (R 2=0.40) but no relationship was found during the non-reproductive period.

Keywords

Reproductive Season Somatic Growth Individual Biomass Copepod Production Copepodites Stage Versus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

We would like to thank the crew of the Sepia and Squilla for their help with sample collection. We also thank Dr D. Bonnet and Dr A. G. Hirst for their comments on the manuscript. This work was supported by a grant from the Spanish Ministry of Education, Culture and Sport to L. Yebra. Completion was funded by the NERC Marine Productivity Thematic Programme (grant NE/C508418/1). This work is a contribution to the Plymouth Marine Laboratory Core Strategic Research Programme.

References

  1. Bergeron JP, Alayse-Danet AM (1981) Aspartate transcarbamylase de la coquille Saint-Jacques Pecten maximus L. (mollusque lamelibranche): méthode de dosage et variations de l’activité dans le manteau et la gonade. J Exp Mar Biol Ecol 50:99–117CrossRefGoogle Scholar
  2. Biegala IC, Harris RP (1999) Sources of seasonal variability in mesozooplankton aspartate transcarbamylase activity in coastal waters off Plymouth, UK. J Plankton Res 21:2085–2103CrossRefGoogle Scholar
  3. Biegala IC, Harris RP, Bergeron JP (1999) ATCase activity, RNA:DNA ratio, gonad development stage, and egg production in the female copepod Calanus helgolandicus. Mar Biol 135:1–10CrossRefGoogle Scholar
  4. Corkett CJ, McLaren IA, Sevigny J-M (1986) The rearing of the marine calanoid copepods Calanus finmarchicus (Gunnerus), C. glacialis Jaschnov and C. hyperboreus Krøyer with comment on the equiproportional rule. Syllogeus 58:539–546Google Scholar
  5. Green EP, Harris RP, Duncan A (1993) The seasonal abundance of the copepodites stages of Calanus helgolandicus and Pseudocalanus elongatus off Plymouth. J Mar Biol Ass UK 73:109–122CrossRefGoogle Scholar
  6. Heinle DR (1966) Production of the calanoid copepod, Acartia tonsa, in the Patuxent River estuary. Chesapeake Sci 7:59–74CrossRefGoogle Scholar
  7. Hernández-León S, Almeida C, Montero I (1995) The use of aspartate transcarbamylase activity to estimate growth rates in zooplankton. ICES J Mar Sci 52:377–383CrossRefGoogle Scholar
  8. Hirst AG, Bunker A (2003) Growth of marine planktonic copepods: global rates and patterns in relation to chlorophyll a, temperature, and body weight. Limnol Oceanogr 48(5):1988–2010CrossRefGoogle Scholar
  9. Hirst AG, Lampitt RS (1998) Towards a global model of in situ weight-specific growth in marine planktonic copepods. Mar Biol 132:247–257CrossRefGoogle Scholar
  10. Hirst AG, McKinnon AD (2001) Does egg production represent adult female copepod growth? A call to account for body weight changes. Mar Ecol Prog Ser 223:179–199CrossRefGoogle Scholar
  11. Hirst AG, Roff JC, Lampitt RS (2004) A synthesis of growth rates in marine epipelagic invertebrate zooplankton. Adv Mar Biol 44:1–142Google Scholar
  12. Huntley ME, Lopez MDG (1992) Temperature-dependent production of marine copepods: a global synthesis. Am Nat 140:201–242CrossRefPubMedGoogle Scholar
  13. Irigoien X, Harris RP (2003) Interannual variability of Calanus helgolandicus in the English Channel. Fish Oceanogr 12:317–326CrossRefGoogle Scholar
  14. Jones ME (1980) Pyrimidine nucleotide biosynthesis in animals: genes, enzymes, and regulation of UMP biosynthesis. A Rev Biochem 49:253–279CrossRefGoogle Scholar
  15. Kiørboe T, Sabatini M (1995) Scaling of fecundity, growth and development in marine planktonic copepods. Mar Ecol Prog Ser 122:135–145CrossRefGoogle Scholar
  16. Lowry PH, Rosenbrough NJ, Farr AL, Randall RJ (1951) Protein measurement with a Folin phenol reagent. J Biol Chem 193:265–275PubMedGoogle Scholar
  17. Pond D, Harris R, Head R, Harbour D (1996) Environmental and nutritional factors determining seasonal variability and egg viability of Calanus helgolandicus in coastal waters off Plymouth, UK. Mar Ecol Prog Ser 143:45–63CrossRefGoogle Scholar
  18. Postel L, Fock H, Hagen W (2000) Biomass and abundance. In: Harris RP, Wiebe PH, Lenz J, Skjoldal HR, Huntley M (eds) ICES Zooplankton Methodology Manual. Academic, London San Diego, pp 83–192Google Scholar
  19. Rey-Rassat C, Irigoien X, Harris R, Head R, Carlotti F (2002a) Growth and development of Calanus helgolandicus reared in the laboratory. Mar Ecol Prog Ser 238:125–138CrossRefGoogle Scholar
  20. Rey-Rassat C, Irigoien X, Harris R, Head R, Carlotti F (2002b) Egg production rates of Calanus helgolandicus females reared in the laboratory: variability due to present and past feeding conditions. Mar Ecol Prog Ser 238:139–151CrossRefGoogle Scholar
  21. Rey-Rassat C, Bonnet D, Irigoien X, Harris R, Head R, Carlotti F (2004) Secondary production of Calanus helgolandicus in the western English Channel. J Exp Mar Biol Ecol 313:29–46CrossRefGoogle Scholar
  22. Runge JA, Roff JC (2000) The measurement of growth and reproductive rates. In: Harris RP, Wiebe PH, Lenz J, Skjoldal HR, Huntley M (eds) ICES zooplankton methodology manual. Academic, London San Diego, pp 401–454Google Scholar
  23. Rutter WJ (1967) Protein determinations in embryos. In: Wilt FH, Wessels NK (eds) Methods in developmental biology. Academic, London, pp 671–684Google Scholar
  24. Shreeve RS, Ward P (1998) Moulting and growth of the early stages of two species of Antarctic calanoid copepod in relation to differences in food supply. Mar Ecol Prog Ser 175:109–119CrossRefGoogle Scholar
  25. Welschmeyer NA (1994) Fluorometric analysis of chlorophyll-a in the presence of chlorophyll-b and pheopigments. Limnol Oceanogr 39:1985–1992CrossRefGoogle Scholar
  26. Yebra L, Hernández-León S (2004) Aminoacyl-tRNA synthetases activity as a growth index in zooplankton. J Plankton Res 26:351–356CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  1. 1.Plymouth Marine Laboratory, Prospect PlacePlymouthUK

Personalised recommendations