Abstract
The morphological expressions of imposex in gastropods have been classified into seven stages (S0–S6) with three types (a, b and c). This typical imposex scheme, however, fails to cover some new findings such as specimens with the proximal vas deferens section passing by the vaginal opening (VDSP) and aborted egg capsules. In the present study, Cantharus cecillei was sampled from the coastal waters of China from May to November in 2003. This species showed all the morphological expressions in the typical imposex scheme with the exception of S1b and S2b. In addition, specimens with VDSP occurred in all stages other than stage 4, and were documented for the first time with scanning electron micrographs or in histological sections. Besides VDSP, which was observed exteriorly, excrescent tissue, e.g. the vas deferens, also originated in the ventral channel or the inner wall of the pallial oviduct was extruded. Based on these new findings, the typical imposex scheme was modified to obtain one valid for C. cecillei. Types a*, b* and c* were added as new types corresponding to types a, b and c, respectively. S4* was also rearranged to correspond to S4; S4*, thus, no longer represented the end of imposex development. The updated scheme was not only valid for describing diverse expressions of imposex development in C. cecillei and Nassarius reticulatus, but could also be generalized to define various forms of VDSP in other species. Histological observation indicated that egg capsules would be blocked by the tissues of the vas deferens section or the extrusion of the pallial oviduct wall. This new mechanism, leading to the sterilization of imposex-affected females, was first conclusively revealed and described in the present scheme.
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Acknowledgements
We thank J. Oehlmann for comments and J.H. Sheng for histological sections. This study was supported by a Grant-in-Aid from the National Natural Sciences Foundation of China (40176029), Developing Marine Economy Project of Guangdong (A200005F02) and Natural Science Fund of Guangdong (000792). The experiments comply with the current laws of China where the experiments were performed.
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Communicated by T. Ikeda, Hakodate
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Shi, H., Huang, C., Yu, X. et al. An updated scheme of imposex for Cantharus cecillei (Gastropoda: Buccinidae) and a new mechanism leading to the sterilization of imposex-affected females. Marine Biology 146, 717–723 (2005). https://doi.org/10.1007/s00227-004-1475-7
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DOI: https://doi.org/10.1007/s00227-004-1475-7