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Marine Biology

, Volume 144, Issue 4, pp 757–767 | Cite as

High genetic diversities and complex genetic structure in an Indo-Pacific tropical reef fish (Chlorurus sordidus): evidence of an unstable evolutionary past?

  • L. K. BayEmail author
  • J. H. Choat
  • L. van Herwerden
  • D. R. Robertson
Research Article

Abstract

Historical sea level fluctuations have influenced the genetic structure and evolutionary history of marine species and examining widespread species across their species’ ranges may elucidate some of these effects. Chlorurus sordidus is a common and widespread parrotfish found on coral reefs throughout the Indo-central Pacific. We used phylogenetic, phylogeographic, and cladistic analyses to examine the genetic composition and population structure of this species across most of its latitudinal range limits. We sequenced 354 bp of the mitochondrial control region I in 185 individuals from nine populations. Populations of C. sordidus displayed high levels of genetic diversity, similar to those recorded for widespread pelagic fish species, but much greater nucleotide diversity values than those previously recorded for other demersal reef fishes. Both phylogenetic and phylogeographic analyses detected strong genetic subdivision at the largest spatial scale (i.e. among oceans). The Pacific Ocean was characterised by weak population genetic structure. Separation of the Hawaiian location from other Pacific and West Indian Ocean sites was evident in phylogenetic analyses, but not from analysis of molecular variance. NCA and isolation-by-distance tests suggested that the genetic structure of this species was the result of multiple contemporary and historical processes, including long-distance colonisation and range expansion arising from fluctuating sea levels, limited current gene flow, and isolation by distance. This pattern is to be expected when historically fragmented populations come into secondary contact. We suggest the patterns of population genetic structure recorded in C. sordidus are caused by large local population sizes, high gene flow, and a recent history of repeated fragmentation and remixing of populations resulting from fluctuating sea levels.

Keywords

Gene Flow Genetic Structure Reef Fish Great Barrier Reef Reef Fish Species 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

We thank three anonymous reviewers for constructive comments on a previous draft of this manuscript. Logistic support for sampling over the nine geographic locations was provided as follows: Amirante island reefs and the granitic reefs of the Seychelles Bank, Seychelles Fisheries Authority, research vessel “L’Amitie”; Rota Island, the University of Guam Marine Laboratory; Hawaii, University of Hawaii Marine Laboratory; PNG, Mahonia Na Dari Research Centre, Kimbe Bay; Abrolhos Islands, West Australia Fisheries (Geraldton); Ningaloo Australian Institute of Marine Science (West Australia); Lizard Island, Lizard Island Research Station, (Australian Museum); Whitsunday Islands, Australian Institute of Marine Science. Funding was provided by the Queensland Government/Smithsonian Institution collaborative research program (JHC, DRR), Smithsonian Tropical Research Institution, Panama, and the James Cook University funding program (ARC small grant). We thank C. Dudgeon, J. Gardiner, and S. Klanten for logistic and intellectual support in the laboratory, M. van Oppen, D. Blair, G. Worheide, and D. Posada for statistical advice, and J. Ackerman, P. Doherty, D. Bellwood, W. Robbins, M. Meekan, and M. McCormick for additional sample collections. The methods employed in this study comply with current Australian law.

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Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  • L. K. Bay
    • 1
    Email author
  • J. H. Choat
    • 1
  • L. van Herwerden
    • 1
  • D. R. Robertson
    • 2
  1. 1.Molecular Ecology and Evolution Laboratory, School of Marine Biology and AquacultureJames Cook UniversityTownsvilleAustralia
  2. 2.Smithsonian Tropical Research Institute (Panama), STRIUSA

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