Abstract
Some organisms living in coastal bays in Newfoundland and Labrador have to contend with sub-zero temperatures for most of the year. The goal of this study was to examine the lipid composition of the food web in such an environment in order to obtain information on essential nutrients and trophic relations. In August 2000, plankton, 16 species of macroinvertebrates, and sediments were collected in a shallow, sheltered bay, Gilbert Bay, southern Labrador (52°35′N; 55°50′W). Plankton had higher proportions of ω3 fatty acids (39±5%) and lower proportions of bacterial fatty acids (5±2%), while sediments had 12±5% ω3 fatty acids and 15±9% bacterial fatty acids. Plankton ω3 fatty acids levels and sediment bacterial fatty acid levels were higher than in equivalent samples previously collected further south, in Newfoundland. Benthic macroinvertebrates contained 0.5±0.4% of their wet weight as lipid, of which 45±15% was triacylglycerol. Levels of ω3 fatty acids were high and relatively constant across phyla, accounting for 36±11% of the total fatty acids. Compared to other invertebrates, echinoderms had a unique fatty acid composition, which was characterized by high levels of 20:4ω-6 (9±6%), bacterial fatty acids (8±4%), and ∑20:1+22:1 monoenoic fatty acids (13±6%). Baseline lipid data from plankton and macroinvertebrates showed constantly high levels of polyunsaturated fatty acids, indicating their physiological importance at low temperatures.
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References
Ackman RG (1986) WCOT (capillary) gas-liquid chromatography. In: Hamilton RJ, Rossell JB (eds) Analysis of oils and fats. Elsevier, New York, pp 137–206
Ackman RG, Hooper SN (1973) Non-methylene-interupted fatty acids in lipids of shallow-water marine invertebrates: comparison of two molluscs (Littorina littorina and Luna triseriata) with the sand shrimp (Crangon septemspinosus). Comp Biochem Physiol B 46:153–155
Ackman RG, Kean-Howie J (1995) Fatty acids in aquaculture: are ω-3 fatty acids always important? In: Chorm EL, Sessa DJ (eds) Nutrition and utilization technology in aquaculture. AOCS Press, Champaign, IL, USA, pp 82–104
Albers CS, Kattner G, Hagen W (1996) The composition of wax esters, triacylglycerols and phospholipids in Arctic and Antarctic copepods: evidence of energetic adaptations. Mar Chem 55:347–358
Allen WV (1968) Fatty acid synthesis in the echinoderms: Asterias rubens, Echinus esculentus and Holothuria forskali. J Mar Biol Assoc UK 48:521
Alvarez HM, Pucci OH, Steinbuchel A (1997) Lipid storage compounds in marine bacteria. Appl Microbiol Biotechnol 47:132–139
Arts MT, Ackman RG, Holub BJ (2001) "Essential fatty acids" in aquatic ecosystems: a crucial link between diet and human health and evolution. Can J Fish Aquat Sci 58:122–137
Bell MV, Sargent JR (1985) Fatty acid analyses of phospholglycerides from tissues of the clam Chlamys islandica (Muller) and the starfish Ctenodiscus crispatus (Retzius) from Balsfjorden, northern Norway. J Exp Mar Biol Ecol 87:31–40
Berge JP, Gouygou JP, Dubacq JP, Durand P (1995) Reassessment of lipid composition of the diatom, Skeletonema costatum. Phytochemistry 39:1017–1021
Brusca R, Brusca G (1990) Invertebrates. Sinauer, Sunderland, Mass.
Budge SM, Parrish CC (1998) Lipid biogeochemistry of plankton, settling matter and sediments in Trinity Bay, Newfoundland. II. Fatty acids. Org Geochem 29:1547–1559
Budge SM, Parrish CC (1999) Lipid class and fatty acid composition of Pseudo-nitzschia multiseries and Pseudo-nitzschia pungens and effects of lipolytic enzyme deactivation. Phytochemistry 52:561–566
Budge SM, Parrish CC, Thompson RJ, McKenzie CH (2000) Fatty acids in plankton in relation to bivalve dietary requirements. In: Shahidi F (ed) Seafood in health and nutrition. ScienceTech Publishing, St. John's, Canada, pp 495–520
Budge SM, Parrish CC, McKenzie CH (2001) Fatty acid composition of phytoplankton, settling particulate matter and sediments at a sheltered bivalve aquaculture site. Mar Chem 76:285–303
Canuel EA, Martens CS (1996) Reactivity of recently deposited organic matter: degradation of lipid compounds near the sediment–water interface. Geochim Cosmochim Acta 60:1793–1806
Ciapa B, Allemand D, De Renzis G (1995) Effect of arachidonic acid on Na+/H+ exchange and neutral amino acid transport in sea urchin eggs. Exp Cell Res 218:248–254
Colombo JC, Silverberg N, Gearing JN (1997) Lipid biogeochemistry in the Laurentian Trough. II. Changes in composition of fatty acids, sterols and aliphatic hydrocarbons during early diagenesis. Org Geochem 26:257–274
Cossins AR, Lee JA (1985) The adaptation of membrane structure and lipid composition in cod. In: Gilles R (eds) Circulation, respiration, and metabolism. Springer, Berlin Heidelberg New York
Cossins AR, Friedlander MJ, Prosser CL (1997) Correlations between behavioural temperature adaptations of goldfish and the viscosity and fatty acid composition of their synaptic membranes. J Comp Physiol 120:109–121
Delaunay F, Marty Y, Moal J, Samain JF (1992) Growth and lipid class composition of Pecten maximus (L.) larvae grown under hatchery conditions. J Exp Mar Bio Ecol 163:209–219
De Baar HJW, Farrington JW, Wakeham SG (1983) Vertical flux of fatty acids in the North Atlantic Ocean. J Mar Res 41:19–41
de Young B, Sanderson B (1995) The circulation and hydrography of Conception Bay, Newfoundland. Atmos-Ocean 33:135–162
Dunstan GA, Volkman JV, Barrett SM, Leroi J, Jeffrey SW (1994) Essential polyunsaturated fatty acids from 14 species of diatom (Bacillariophyceae). Phytochemistry 35:155–161
Dunstan GA, Olley J, Ratkowsky DA (1999) Major environmental and biological factors influencing the fatty acid composition of seafood from Indo-Pacific to Antarctic waters. Recent Results Dev Lipid Res 3:63–86
Estevez A, McEvoy LA, Bell JG, Sargent JR (1999) Growth, survival, lipid composition and pigmentation of turbot (Scophthalmus maximus) larvae fed live-prey enriched in arachidonic and eicosapentaenoic acids. Aquaculture 180:321–343
Folch J, Lees M, Sloane SG (1957) A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 22:497–509
Fraser AJ, Sargent JR (1989) Formation and transfer of fatty acids in an enclosed marine food chain comprising phytoplankton, zooplankton and herring (Clupea harengus L.) larvae. Mar Chem 27:1–18
Fulco AJ (1983) Fatty acid metabolism in bacteria. Prog Lipid Res 22:133–160
Grahl-Nielsen O, Mjaavatten O (1991) Dietary influence of fatty acid composition of blubber fat of seals as determined by biopsy: a multivariate approach. Mar Biol 110:59–64
Green JM, Wroblewski JS (2000) Movement patterns of Atlantic cod in Gilbert Bay, Labrador: evidence for bay residency and spawning site fidelity. J Mar Biol 80:1077–1085
Hall JM, Parrish CC, Thompson RJ (2000) Importance of unsaturated fatty acids in regulating bivalve and finfish membrane fluidity in response to changes in environmental temperature. In: Shahidi F (ed) Seafood in health and nutrition. ScienceTech Publishing, St. John's, Canada, pp 435–448
Hall JM, Parrish CC, Thompson RJ (2002) Eicosapentaenoic acid regulates scallop (Placopecten magellanicus) membrane fluidity in response to cold. Biol Bull (Woods Hole) 202:201–203
Hama T (1999) Fatty acid composition of particulate matter and photosynthetic products in subarctic and subtropical Pacific. J Plankton Res 21:1355–1372
Hazel JR, Williams EE, Livermore R, Mozingo N (1991) Thermal adaptation in biological membranes: functional significance of changes in phospholipid molecular species composition. Lipids 26:277–282
Henderson RJ, Forrest DAM, Black KD, Park MT (1997) The lipid composition of sealoch sediments underlying salmon cages. Aquaculture 158:69–83
Joseph JD (1982) Lipid composition of marine and estuarine invertebrates, part II: Mollusca. Prog Lipid Res 21:109–153
Joseph JD (1989) Distribution and composition of lipids in marine invertebrates. In: Ackman RG (eds) Marine biogenic lipids, fats, and oils, vol II. CRC Press, Boca Raton, Fla., pp 49–144
Kattner G, Brockmann UH (1990) Particulate and dissolved fatty acids in an enclosure containing a unialgal Skeletonema costatum (Greve) Cleve culture. J Exp Mar Biol Ecol 141:1–13
Kayama M, Araki S, Sato S (1989) Lipids in marine plants. In: Ackman RG (eds) Marine biogenic lipids, fats, and oils, vol II. CRC Press, Boca Raton, Fla., pp 3–48
Leaf A, Weber P (1988) Cardiovascular effects of n-3 fatty acids. N Engl J Med 318:549–557
Libes S (1992) An introduction to marine biogeochemistry. Wiley, New York
Manly BFJ (1986) Multivariate statistical methods: a primer. Chapman and Hall, London
Mayzaud P, Chanut JP, Ackman RG (1989) Seasonal changes of the biochemical composition of marine particulate matter with special reference to fatty acids and sterols. Mar Ecol Prog Ser 56:189–204
Maziane T, Tsuchiya M (2000) Fatty acids as tracers of organic matter in the sediment and food web of a mangrove/intertidal flat ecosystem, Okinawa, Japan. Mar Ecol Prog Ser 200:49–57
Meijer L, Guerrier P, McClouf J (1984) Arachidonic acid, 12- and 15-hydroxyeicosatetraenoic acids, eicosapentaenoic acid, and phospholipase A2 induce starfish oocyte maturation. Dev Biol 106:368–378
Morris C (2000) Biology of a resident cod (Gadus morhua) population in Gilbert Bay, Labrador. MSc thesis, Memorial University of Newfoundland, St. John's
Morris C, Simms JM, Anderson TC (2002) Biophysical overview of Gilbert Bay: a proposed marine protected area in Labrador. Can Manuscr Rep Fish Aquat Sci 2595:1–25
Morrison WR, Smith LM (1964) Preparation of fatty acid methyl esters and dimethylacetals from lipids with boron fluoride methanol. J Lipid Res 5:600–608
Nadeau M, Cliché G (1998) Predation of juvenile sea scallops (Placopecten magellanicus) by crabs (Cancer irroratus and Hyas sp.) and starfish (Asterias vulgaris, Leptasterias polaris, and Crossaster papposus). J Shellfish Res 17:905–910
Navarro JC, McEvoy LA, Amat F, Sargent JR (1995) Effects of diet on fatty acid composition of body zones in larvae of the sea bass Dicentrarchus labrax: a chemometric study. Mar Biol 124:177–183
Newton IS (2000) Long chain fatty acids in health nutrition. In: Shahidi F (ed) Seafood in health and nutrition. ScienceTech Publishing, St. John's, Canada, pp 15–28
Parrish CC (1987) Separation of aquatic lipid classes by Chromarod thin-layer chromatography with measurement by Iatroscan flame ionization detection. Can J Fish Aquat Sci 44:722–731
Parrish CC (1988) Dissolved and particulate marine lipid classes: a review. Mar Chem 23:17–40
Parrish CC (1998) Lipid biogeochemistry of plankton, settling matter and sediments in Trinity Bay, Newfoundland. I. Lipid classes. Org Geochem 29:1531–1545
Parrish CC, McKenzie CH, MacDonald BA, Hatfield EA (1995) Seasonal studies of seston lipids in relation to microplankton species composition and scallop growth in South Broad Cove, Newfoundland. Mar Ecol Prog Ser 129:151–164
Parrish CC, Yang Z, Lau A, Thompson RJ (1996) Lipid composition of Yoldia hyperborea (Protobranchia), Nephthys ciliata (Nephthyidae) and Artacama proboscidea (Terebellida) living at sub-zero temperatures. Comp Biochem Physiol B 114:59–67
Parrish CC, Wells JS, Yang Z, Dabinett P (1998) Growth and lipid composition of scallop juveniles, Placopecten magellanicus, fed the flagellate Isochrysis galbana with varying lipid composition and the diatom Chaetoceros muelleri. Mar Biol 133:461–471
Parrish CC, Abrajano TA, Budge SM, Helleur RJ, Hudson ED, Pulchan K, Ramos C (2000) Lipid and phenolic biomarkers in marine ecosystems: analysis and applications. In: Wangersky PJ (ed) Marine chemisty. Springer, New York Heidelberg Berlin, pp 193–224
Pomeroy LR, Wiebe WJ, Deibel D, Thompson RJ, Rowe GT, Pakulski JD (1991) Bacterial responses to temperature and substrate concentration during the Newfoundland spring bloom. Mar Ecol Prog Ser 75:143–159
Reuss N, Poulsen LK (2002) Evaluation of fatty acids as biomarkers for a natural plankton community. A field study of a spring bloom and a post-bloom period off West Greenland. Mar Biol 141:423–434
Saito H, Kotani Y (2000) Lipids of four boreal species of calanoid copepods: origin of moneone fats of marine animals at higher trophic levels in the grazing food chain in the subarctic ocean ecosystem. Mar Chem 71:69–82
Sargent JR, Falk-Petersen IB, Calder AG (1983) Fatty acid composition of neutral glycerides from the ovaries of asteroids Ctenodiscus crispatus, Asterias lincki and Pteraster militaris from Balsfjorden, northern Norway. Mar Biol 72:257–264
Sargent JR, Eilertsen HC, Falk-Petersen S, Taasen JP (1985) Carbon assimilation and lipid production in phytoplankton in northern Norwegian fjords. Mar Biol 85:109–116
Sargent JR, Parkes RJ, Mueller-Harvey I, Henderson RJ (1987) Lipid biomarkers in marine ecology. In: Sleigh MA (ed) Microbes in the sea. Horwood, Chichester, UK, pp 119–138
Sargent JR, Bell MV, Henderson RJ, Tocher DR (1989) The lipids. In: Halver J (eds) Fish nutrition. Academic, New York, pp 153–218
Shick JM, Edwards KC, Dearborn JH (1981) Physiological ecology of the deposit-feeding sea star Ctenodiscus crispatus: ciliated surfaces and animal–sediment interactions. Mar Ecol Prog Ser 5:165–184
Silvert W (1992) Assessing environmental impacts of finfish aquaculture in marine waters. Aquaculture 107:67–79
Skerratt JH, Nichols PD, McMeekin TA, Burton H (1995) Seasonal and inter-annual changes in planktonic biomass and community structure in eastern Antarctica using signature lipids. Mar Chem 51:93–113
Stanley DW, Howard RW (1998) The biology of prostaglandins and related eicosanoids in invertebrates: cellular, organismal and ecological actions. Am Zool 38:369–381
Sukenik A, Wahnon R (1991) Biochemical quality of marine unicellular algae with special emphasis on lipid composition. 1. Isochrysis galbana. Aquaculture 97:61–72
Sun MY, Aller RC, Lee C, Wakeham SG (1999) Enhanced degradation of algal lipids by benthic macrofaunal activity: effect of Yoldia limatula. J Mar Res 57:775–804
Thompson PA, Harrison PJ, Whyte JNC (1990) Influence of irradiance on the fatty acid composition of phytoplankton. J Phycol 26:278–288
Thompson PA, Guo MX, Harrison PJ, Whyte JNC (1992) Effects of variation in temperature. II. On the fatty acid composition of eight species of marine phytoplankton. J Phycol 28:488–497
Vogt NB, Moksness E, Sporstoel SP, Knutsen H, Nordenson S, Kolset K (1986) SIMCA principal component analysis of fatty acid patterns in Day-1 and Day-8 cod (Gadus morhua ) and haddock (Melanogrammus aeglefinus ) eggs. Mar Biol 92:173–182
Wakeham SG, Lee C, Farrington JW, Gagosian RB (1984) Biogeochemistry of particulate organic matter in the oceans: results from sediment trap experiments. Deep-Sea Res 31:509–528
Whyte JNC, Bourne N, Ginther NG (1991) Depletion of nutrient reserves during embryogenesis in the scallop Patinopecten yessoensis (Jay). J Exp Mar Biol Ecol 149:67–79
Wroblewski JS, Bailey WL, Russell J (1998) Grow-out cod farming in southern Labrador. Bull Aquacult Assoc Can 98:47–49
Acknowledgements
We wish to thank Dr. J. Wroblewski, Dr. C. McKenzie, Dr. T. Avery, J. Wells, and S. Fraser for help with field sampling, phytoplankton identification, shipping logistics, computer assistance, and nutrient analysis. "Coasts Under Stress", a Canadian collaborative research program, NSERC, and SSHRC funded this work.
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Communicated by J.P. Grassle, New Brunswick
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Copeman, L.A., Parrish, C.C. Marine lipids in a cold coastal ecosystem: Gilbert Bay, Labrador. Marine Biology 143, 1213–1227 (2003). https://doi.org/10.1007/s00227-003-1156-y
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DOI: https://doi.org/10.1007/s00227-003-1156-y