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Between-individual variation in haemocyanin concentrations in the Norway lobster Nephrops norvegicus following exposure to hypoxia and manganese

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Abstract

Environmental hypoxia and the release of reduced manganese (Mn) are often combined stress factors in marine sediments. Previous investigations have shown that hypoxia induces variable responses in the concentration of the respiratory pigment haemocyanin (Hc) of crustaceans. A recent study demonstrated that the magnitude and direction of the changes was dependent on the initial level of Hc in individuals of the Norway lobster Nephrops norvegicus. The changes also took place within 24 h rather than days as suggested by previous studies. In this present study we investigated changes of Hc in individual N. norvegicus in uncontaminated sea water after exposure to and release from realistic hypoxic stress (PO2=6.0 kPa). Furthermore we investigated how concomitant exposure to realistic concentrations of manganese (20 mg l-1) modified the responses we observed. We confirmed a between-individual variation in response and a change in Hc towards an optimum level after hypoxia, but also showed that changes that took place post-hypoxic exposure occurred over a timescale similar to that of the hypoxic response itself. Manganese exposure resulted in no significant changes in Hc whereas Mn exposure combined with hypoxia resulted in a significant decrease (15%) in all individuals independent of initial Hc. N. norvegicus was thus unable to compensate for the effects of hypoxia by synthesis of Hc after exposure to naturally occurring Mn concentrations.

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Acknowledgements

This research was supported by FORMAS (The Swedish Research Council for Environment, Agricultural Sciences and Spatial Planning) no. 40.0182/99) to S.P.B. and an LSF/TMR (no. 13) award to J.I.S.

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Authors and Affiliations

  1. Department of Marine Ecology, Kristineberg Marine Research Station, Göteborg University, 450 34 Fiskebäckskil, Sweden

    S. P. Baden & C. L. J. Håkansson

  2. School of Biological Sciences, University of Plymouth, Drake Circus, Plymouth, PL4 8QQ, UK

    J. I. Spicer

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  1. S. P. Baden
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  2. C. L. J. Håkansson
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  3. J. I. Spicer
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Communicated by L. Hagerman, Helsingør

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Baden, S.P., Håkansson, C.L.J. & Spicer, J.I. Between-individual variation in haemocyanin concentrations in the Norway lobster Nephrops norvegicus following exposure to hypoxia and manganese. Marine Biology 143, 267–273 (2003). https://doi.org/10.1007/s00227-003-1082-z

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