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Change in the uptake and cellular Si:N ratio in diatoms responding to the ambient Si:N ratio and growth phase

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Abstract

A change in the Si:N ratio of diatom cells during growth was examined for Chaetoceros socialis and Thalassiosira sp., with different initial silicate to nitrate (Si:N) ratios in the media. During exponential growth, C. socialis assimilated silicate and nitrate with a molar ratio of 0.5, independent of the ratio in the media, but after the depletion of nitrate, silicate continued to be taken up, and the Si:N ratio in the stationary phase increased to 2 as a function of the Si:N ratios in the media. In contrast, the ratio of silicate to nitrate taken up by Thalassiosira sp. increased with an increase in the Si:N ratio in the media. The Si:N ratio in the cells during the stationary phase increased in response to an increase in this ratio in the media. The Si:chl a ratio also increased with the increase in the initial Si:N ratio in the media, while the N:chl a ratio did not change to a great extent, indicating the changes in the cellular Si:N ratio was derived from changes in the Si content of the cells. These results indicated that the cellular Si:N ratio changed with the Si:N ratio in the medium, and the Si:N uptake ratio during the growth phase was different depending on diatom species. Thus, the dominance of different diatom species may affect nutrient composition and dynamics in the ocean.

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Acknowledgements

The author expresses special thanks to Dr. P.J. Harrison for reading a draft of the manuscript and for helpful comments. This manuscript was greatly improved by the comments of anonymous reviewers.

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  1. Graduate School of Fisheries Science, Hokkaido University, Hakodate, Japan

    I. Kudo

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  1. I. Kudo
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Correspondence to I. Kudo.

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Communicated by T. Ikeda, Hakodate

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Kudo, I. Change in the uptake and cellular Si:N ratio in diatoms responding to the ambient Si:N ratio and growth phase. Marine Biology 143, 39–46 (2003). https://doi.org/10.1007/s00227-003-1063-2

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  • DOI: https://doi.org/10.1007/s00227-003-1063-2

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