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The digestive protease, chymotrypsin, as an indicator of nutritional condition in larval red drum (Sciaenops ocellatus)

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Abstract

Laboratory-reared red drum (Sciaenops ocellatus) larvae were used to evaluate the potential of chymotrypsin as an indicator of nutritional condition in marine fish larvae. The response of chymotrypsin activity to food deprivation and reductions in nutrient intake was determined. Enzyme activity declined rapidly to undetectable levels in food-deprived larvae 6–18 days old. Larvae fed poor-quality live prey (starved rotifers, Brachionus plicatilis) exhibited reductions in growth (18%) and enzyme activity (84%) relative to larvae fed high-quality prey (enriched rotifers). Potential sources of variation in chymotrypsin activity unrelated to nutritional status, including diel periodicity, and exogenous enzymes sources were examined. A diel pattern in chymotrypsin activity was detected with an 8.7-fold increase in activity occurring from low to high points during a 24-h period. Highest activity levels occurred late in the day (1600 hours) and lowest activity in the morning prior to feeding (0800 hours). The estimated contribution of exogenous enzymes from prey in the digestive tract to measurements of larval enzyme activity was small, reaching a maximum of 4.1% on day 18 in well-fed larvae. Results indicate that exogenous enzymes will not lead to the misclassification of larvae in poor condition. A relationship between chymotrypsin activity and standard length was established for well-fed and food-deprived larvae that could potentially be used to determine the nutritional condition of wild-caught larvae.

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Acknowledgements

The authors wish to thank Juan Pablo Lazo, Sharon Herzka, Cynthia Faulk, and Rafael Perez for assistance and helpful suggestions. This work was supported by grants from the Sid W. Richardson Foundation and the Texas Higher Education Coordinating Board Advanced Research Program. The experiments presented here comply with current laws of the United States.

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  1. University of Texas at Austin Marine Science Institute, 750 Channel View Drive, Port Aransas, TX 78373, USA

    S. L. Applebaum & G. J. Holt

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  1. S. L. Applebaum
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  2. G. J. Holt
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Correspondence to G. J. Holt.

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Communicated by P.W. Sammarco, Chauvin

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Applebaum, S.L., Holt, G.J. The digestive protease, chymotrypsin, as an indicator of nutritional condition in larval red drum (Sciaenops ocellatus). Marine Biology 142, 1159–1167 (2003). https://doi.org/10.1007/s00227-003-1041-8

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  • DOI: https://doi.org/10.1007/s00227-003-1041-8

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