Skip to main content

Phase-retrieval-based synchrotron X-ray micro-tomography for 3D structural characterization and quantitative analysis of agalloch eaglewood

Abstract

The propagation-based phase-contrast computed micro-tomography (PPCT) dominates the nondestructive three-dimensional inner structure measurement in biological and soft material research, especially for the synchrotron-based application. It is essential for botanic assessments in plant physiology and eco-physiology to quantitatively visualize microstructures and tiny density variations in wood tissues. An experimental study of synchrotron-based X-ray PPCT combined with the presented phase-attenuation-duality phase retrieval (PR) algorithm was implemented with the agalloch eaglewood specimens at different stages. Due to the striking contrast-to-noise ratio and density resolution of the PR-PPCT technique, the experimental results successfully visualized the three-dimensional morphological characterization of different tissues of agalloch eaglewood specimens, and the feature distribution of eaglewood resin was quantitatively segmented and measured for statistic analysis of component volume and size of eaglewood growth. It was demonstrated that the PR-PPCT technique has a great potential for 3D visualization and quantitative analysis of structural characteristics of agalloch eaglewood, associated with resin formation and distribution in specimens, and it is helpful for the wood science and application.

This is a preview of subscription content, access via your institution.

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8
Fig. 9

References

  1. Barden A (2000) Heart of the matter: agarwood use and trade and CITES implementation for Aquilaria malaccensis. Traffic International, Cambridge. ISBN 1-85850-177-6

    Google Scholar 

  2. Broad S (1995) Agarwood harvesting in Vietnam. Traffic Bull 88:15–96

    Google Scholar 

  3. Chen H-Q, Wei J-H, Yang J-S, Zhang Z, Yang Y, Gao Z-H, Sui C, Gong B (2012) Chemical constituents of agarwood originating from the endemic genus Aquilaria plants. Chem Biodivers 9(2):236–250

    CAS  Article  PubMed  Google Scholar 

  4. Cnudde V, Boone MN (2013) High-resolution X-ray computed tomography in geosciences: a review of the current technology and applications. Earth Sci Rev 123:1–17

    Article  Google Scholar 

  5. Davis JR, Lerdin A, Wells P, Ilic J (1991) X-ray microtomography of wood. J Inst Wood Sci 12:259–261

    Google Scholar 

  6. Groso A, Abela R, Stampanoni M (2006) Implementation of a fast method for high resolution phase contrast tomography. Opt Express 14:8103–8110

    CAS  Article  PubMed  Google Scholar 

  7. Guigay JP, Langer M, Boistel R, Cloetens P (2007) Mixed transfer function and transport of intensity approach for phase retrieval in the Fresnel region. Opt Lett 32:1617–1619

    Article  PubMed  Google Scholar 

  8. Gureyev TE, Pogany A, Paganin DM, Wilkins SW (2004) Linear algorithms for phase retrieval in the Fresnel region. Opt Commun 231:53–70

    CAS  Article  Google Scholar 

  9. Gureyev TE, Paganin DM, Myers GR, Nesterets YI, Wilkins SW (2006) Phase-and-amplitude computer tomography. Appl Phys Lett 89:034102

    Article  Google Scholar 

  10. Lancaster C, Espinoza E (2012) Evaluating agarwood products for 2-(2-phenylethyI) chromones suing direct analysis in real time time-of-flight mass spectrometry. Rapid Commun Mass Spectrom 26(23):2649–2656

    CAS  Article  PubMed  Google Scholar 

  11. Lang S, Zanette I, Dominietto M, Langer M, Rack A, Schulz G, Le Duc G, David C, Mohr J, Pfeiffer F, Muller B, Weitkamp T (2014) Experimental comparison of grating- and propagation-based hard X-ray phase tomography of soft tissue. J Appl Phys 116:154903

    Article  Google Scholar 

  12. Langer M, Cloetens P, Peyrin F (2010) Regularization of phase retrieval with phase-attenuation duality prior for 3-D holotomography. IEEE Trans Image Process 19(9):2428–2436

    Article  PubMed  Google Scholar 

  13. Liu HQ, Ren Y, Guo H, Xue Y, Xie H, Xiao TQ, Wu X (2012) Phase retrieval for hard X-ray computed tomography of samples with hybrid compositions. Chin Opt Lett 10:121101

    Article  PubMed  PubMed Central  Google Scholar 

  14. Liu Y, Nelson J, Holzner C, Andrews JC, Pianetta P (2013) Recent advances in Synchrotron-based hard X-ray phase contrast imaging. J Phys D Appl Phys 46:494001

    Article  Google Scholar 

  15. Liu HQ, Wu XZ, Xiao TQ (2015a) Optimization of reconstructed quality of hard X-ray phase micro-tomography. Appl Opt 54:5610

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  16. Liu HQ, Wu XZ, Xiao TQ (2015b) Technical Note: Synchrotron-based high energy X-ray phase sensitive micro-tomography for biomedical research. Med Phys 42(10):5595–5603

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  17. Liu HQ, Xiao TQ, Xie H, Fu Y, Zhang X, Fan X (2017) Nondestructive material characterization of meteorites with synchrotron-based high energy X-ray phase micro-computed tomography. J Phys D Appl Phys 50:055301

    Article  Google Scholar 

  18. Long C, Li S, Long B, Shi Y, Liu B (2009) Medical plants used by the Yi ethnic group: a case study in central Yunnan. J Ethnobiol Ethnomed 5:13

    Article  PubMed  PubMed Central  Google Scholar 

  19. Maire E, Withers PJ (2014) Quantitative X-ray tomography. Int Mater Rev 59(1):1–12

    CAS  Article  Google Scholar 

  20. Mayo SC, Chen F, Evans R (2010) Micron-scale 3D imaging of wood and plant microstructure using high-resolution X-ray phase-contrast microtomography. J Struct Biol 171:182–188

    CAS  Article  PubMed  Google Scholar 

  21. Myers GR, Gureyev TE, Paganin DM (2007) Stability of phase-contrast tomography. J Opt Soc Am A 24:2516

    Article  Google Scholar 

  22. Naef R (2011) The volatile and semi-volatile constituents of agarwood, the infected heartwood of Aquilaria species: a review. Flavour Fragr J 26(2):73–87

    CAS  Article  Google Scholar 

  23. Ng LT, Chang YS, Kadir AA (1997) A review on agar (gaharu) producing Aquilaria species. J Trop For Prod 2(2):272–285

    Google Scholar 

  24. Paganin D, Mayo SC, Gureyev TE, Miller PR, Wilkins SW (2002) Simultaneous phase and amplitude extraction from a single defocused image of a homogeneous object. J Microsc 206:33–40

    CAS  Article  PubMed  Google Scholar 

  25. Trtik P, Dual J, Keunecke D, Mannes D, Niemz P, Stahli P, Kaestner A, Groso A, Stampanoni M (2007) 3D imaging of microstructure of spruce wood. J Struct Biol 159:46–55

    CAS  Article  PubMed  Google Scholar 

  26. Wilkins SW, Gureyev TE, Gao D, Pogany A, Stevenson AW (1996) Phase contrast imaging using polychromatic hard X-rays. Nature 384:335–338

    CAS  Article  Google Scholar 

  27. Wu X, Liu H (2003) A general theoretical formalism for X-ray phase contrast imaging. J X-ray Sci Technol 11:33–42

    Google Scholar 

  28. Wu X, Liu H, Yan A (2005) X-ray phase-attenuation duality and phase retrieval. Opt Lett 50(4):379–381

    Article  Google Scholar 

  29. Zhou M, Wang H, Suolangjaba Kou J, Yu B (2008) Antinociceptive and anti-inflamatory activities of Aquilaria sinensis Gilg. Leaves extract. J Ethnopharmacol 117:345–350

    Article  PubMed  Google Scholar 

  30. Zhou T, Larsson DH, Stampanoni M, Hertz HM (2014) Comparison of propagation-based X-ray phase-contrast imaging techniques with a liquid-metal-jet source. Proc SPIE 9033(1):131–135

    Google Scholar 

Download references

Acknowledgements

The authors would like to thank the staff of BL13W1 at SSRF. This work was supported by the National Natural Science Foundation of China (Grant Nos. 81660306, 11475248, 11505277).

Author information

Affiliations

Authors

Corresponding authors

Correspondence to Huiqiang Liu or Haigang Liu.

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Liu, H., Lin, F., Lin, J. et al. Phase-retrieval-based synchrotron X-ray micro-tomography for 3D structural characterization and quantitative analysis of agalloch eaglewood. Wood Sci Technol 52, 839–854 (2018). https://doi.org/10.1007/s00226-018-1004-3

Download citation