Abstract
Together with diabetic osteoporosis (DOP), diabetes patients experience poor peri-implant osteogenesis following implantation for dentition defects. Zoledronate (ZOL) is widely used to treat osteoporosis clinically. To evaluate the mechanism of ZOL for the treatment of DOP, experiments with DOP rats and high glucose-grown MC3T3-E1 cells were used. The DOP rats treated with ZOL and/or ZOL implants underwent a 4-week implant-healing interval, and then microcomputed tomography, biomechanical testing, and immunohistochemical staining were performed to elucidate the mechanism. In addition, MC3T3-E1 cells were maintained in an osteogenic medium with or without ZOL to confirm the mechanism. The cell migration, cellular actin content, and osteogenic differentiation were evaluated by a cell activity assay, a cell migration assay, as well as alkaline phosphatase, alizarin red S, and immunofluorescence staining. The mRNA and protein expression of adenosine monophosphate-activated protein kinase (AMPK), phosphorylated AMPK (p-AMPK), osteoprotegerin (OPG), receptor activator of nuclear factor kappa B ligand (RANKL), bone morphogenetic protein 2 (BMP2), and collagen type I (Col-I) were detected using real-time quantitative PCRs and western blot assays, respectively. In the DOP rats, ZOL markedly improved osteogenesis, enhanced bone strength and increased the expression of AMPK, p-AMPK, and Col-I in peri-implant bones. The in vitro findings showed that ZOL reversed the high glucose-induced inhibition of osteogenesis via the AMPK signaling pathway. In conclusion, the ability of ZOL to promote osteogenesis in DOP by targeting AMPK signaling suggests that therapy with ZOL, particularly simultaneous local and systemic administration, may be a unique approach for future implant repair in diabetes patients.
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Funding
This study was supported by the National Natural Science Foundation of China (Grant No. 81600844); Science and Technology Project of Hebei Education Department (Grant No. QN2020438); Natural Science Foundation of Hebei Province (Grant No. H2017209114).
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YZ: conceptualization, methodology, investigation, data curation, writing—original draft. SJ: conceptualization, methodology, investigation, data curation, writing—original draft. GW: methodology, data curation, software, visualization. SX: methodology, data curation. ZS: methodology. MQ: data curation. YL: methodology. WB: resources, funding acquisition. WD: conceptualization, writing—review & editing, supervision, project administration, funding acquisition.
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Wei Dong received grants from the Science and Technology Project of Hebei Education Department (Grant No. QN2020438) and the Natural Science Foundation of Hebei Province (Grant No. H2017209114). Wenjuan Bi received grants from the National Natural Science Foundation of China (Grant No. 81600844). Yan Zhang, Shunyi Jia, Guochen Wen, Shanen Xie, Zhiqiang Song, Mengchun Qi and Yongqiang Liang declare that they have no conflict of interest to report.
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The study was approved by the Animal Experimental Ethical Committee of North China University of Science and Technology (No. LX2021106) and performed in accordance with the regulations and codes of practice for laboratory animal management and ethical requirements.
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Zhang, Y., Jia, S., Wen, G. et al. Zoledronate Promotes Peri-Implant Osteogenesis in Diabetic Osteoporosis by the AMPK Pathway. Calcif Tissue Int 113, 329–343 (2023). https://doi.org/10.1007/s00223-023-01112-0
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DOI: https://doi.org/10.1007/s00223-023-01112-0