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Strontium and Bone Nanostructure in Normal and Ovariectomized Rats Investigated by Scanning Small-Angle X-Ray Scattering

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Abstract

The effect of SrCl2 treatment on bone nanostructure in a rat ovariectomy model was studied using scanning small-angle X-ray scattering (sSAXS). Twelve 6-month-old female Wistar rats were used. Six animals were ovariectomized (+ovx) and six were left intact after sham surgery (−ovx). Six animals, three +ovx and three −ovx, were treated with 4 mmol SrCl2 (aq)/kg/day (+Sr), whereas the remaining six received placebo (−Sr) for 140 days. Rats were labeled with flourochromes at days 7, 126, and 136. Femoral cross sections were studied using fluorescence microscopy, scanning electron microscopy including energy-dispersive X-ray analysis, and sSAXS. The SAXS data comprised about 5,500 measurements and provided information about mineral crystal thickness and orientation in new and old bone. The newly formed bone contained higher levels of Sr2+ in +Sr than in −Sr animals, indicating that the Sr2+ was incorporated into the new bone. Mineral plates were significantly thicker in old bone, 2.62 nm (95% CI 2.58–2.66), than in new bone, 2.41 nm (95% CI 2.36–2.46). Surprisingly, mineral plates in new bone were significantly thicker (2.52 [95% CI 2.47–2.57] nm vs. 2.41 [95% CI 2.36–2.46] nm, P = 0.017) in +ovx rats than in −ovx rats. However, no significant effect of SrCl2 on mineral plate thicknesses in new bone was observed. The statistical model yielded estimates of the difference in bone mineral plate thickness induced by Sr. The estimated effect of Sr was −0.09 (95% CI −0.21 to 0.03) and 0.02 (95% CI −0.10 to 0.14) nm for new bone in −ovx and +ovx rats, respectively.

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Acknowledgements

We thank Jacques Chevallier at the Institute of Physics and Astronomy, University of Aarhus, for his kind technical assistance. H. B. thanks the Danish Natural Sciences Research Council for funding through a Skou research associate professor fellowship. Hanne Broch at the Institute of Anatomy is gratefully acknowledged for handling the animals.

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Authors and Affiliations

  1. Department of Endocrinology and Metabolism C, Aarhus University Hospital, Århus, Denmark

    Mathias H. Bünger & Bente L. Langdahl

  2. Interdisciplinary Nanoscience Center, University of Aarhus, Århus, Denmark

    Mathias H. Bünger, Toke K. Hansen, Søren Sørensen, Flemming Besenbacher, Jan S. Pedersen & Henrik Birkedal

  3. Department of Connective Tissue Biology, Institute of Anatomy, University of Aarhus, Århus, Denmark

    Hans Oxlund & Jesper S. Thomsen

  4. Department of Chemistry, University of Aarhus, 140 Langelandsgade, 8000, Århus C, Denmark

    Toke K. Hansen, Søren Sørensen, Jan S. Pedersen & Henrik Birkedal

  5. Department of Biostatistics, University of Aarhus, Århus, Denmark

    Bo M. Bibby

  6. Department of Physics and Astronomy, University of Aarhus, Århus, Denmark

    Flemming Besenbacher

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  1. Mathias H. Bünger
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Correspondence to Henrik Birkedal.

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Bünger, M.H., Oxlund, H., Hansen, T.K. et al. Strontium and Bone Nanostructure in Normal and Ovariectomized Rats Investigated by Scanning Small-Angle X-Ray Scattering. Calcif Tissue Int 86, 294–306 (2010). https://doi.org/10.1007/s00223-010-9341-8

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