To investigate differences in the metabolome between ancient and modern Triticum varieties, a total of 15 varieties each of bread and durum wheat, spelt, emmer, and einkorn were cultivated and harvested at four different locations (Seligenstadt, Hohenheim, Eckartsweier, and Oberer Lindenhof), and their acetonitrile extracts were analyzed by means of UPLC–TOF MSe. Nine marker metabolites were clearly identified using reference or surrogate standard compounds including di- and triacylglycerides as well as galactolipids and enabling the discrimination of wheat species and their growing location.
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This research project was supported by the German Ministry of Economics and Energy (via AiF) and the FEI (Forschungskreis der Ernährungsindustrie e.V., Bonn). Project AiF18355 N. The authors specially thank the State Plant Breeding Institute (University of Hohenheim, Stuttgart) for cultivating and providing the wheat samples used in this study.
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Stark, T.D., Weiss, P., Friedrich, L. et al. The wheat species profiling by non-targeted UPLC–ESI–TOF-MS analysis. Eur Food Res Technol 246, 1617–1626 (2020). https://doi.org/10.1007/s00217-020-03517-9
- Non-targeted metabolomics
- Triticum species