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European Food Research and Technology

, Volume 244, Issue 5, pp 861–871 | Cite as

Screening of mushrooms bioactivity: piceatannol was identified as a bioactive ingredient in the order Cantharellales

  • Efstathios P. Vasdekis
  • Athanassios Karkabounas
  • Ioannis Giannakopoulos
  • Dimitrios Savvas
  • Marilena E. Lekka
Original Paper
  • 142 Downloads

Abstract

Wild edible mushroom species are appreciated for consumption due to their high nutritional value. The aim of the present study was to examine in vitro beneficial bioactivity of mushroom extracts and to investigate the molecular identity of the active ingredients. In this regard, methanol extracts of 29 different wild edible mushroom species, that are traditionally consumed by residents in the National Park of North Pindos in North-Western Greece, were examined for antioxidant, antiproliferative, cytotoxic, and pro-apoptotic activities towards a human lung adenocarcinoma cell line A549 by the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay and by flow cytometry. Certain mushroom species exhibited high antioxidant activity, which was related to their high content in total phenols and flavonoids. Methanol extracts of Cantharellus cibarius, Cantharellus cinereus, Craterellus cornucopioides and Hydnum repandum, which belong to the order Cantharellales, exhibited high cytotoxicity and induced apoptosis–necrosis to A549 cells. High Performance Liquid Chromatography (HPLC) coupled with Mass Spectrometry analysis revealed as an active ingredient piceatannol ((E)-4-[2-(3,5-dihydroxyphenyl)ethenyl]1,2-benzenediol-3,3′,4,5′-tetrahydroxy-trans-stilbene). Piceatannol, according to our best knowledge, is identified for the first time in wild edible mushrooms. Experiments with authentic piceatannol confirmed the potent antiproliferative activity of this compound. Tested mushrooms are promising sources of bioactive compounds.

Keywords

Mushrooms Cantharellales Antiproliferative activity Cytotoxic and pro-apoptotic activities Piceatannol 

Notes

Acknowledgements

The authors would like to thank the Unit of Environmental, Organic and Biochemical high-resolution analysis-ORBITRAP-LC–MS of the University of Ioannina for providing access to the facilities. The authors would like to thank the Unit for technical infrastructure, characterization and testing of bioactive substances of the University of Ioannina for providing access to the facilities. The authors would like to thank the OPENSCREEN-GR network for providing access to the facilities.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Compliance with ethics requirements

This article does not contain any studies with human or animal subjects.

References

  1. 1.
    Wasser SP (2002) Medicinal mushrooms as a source of antitumor and immunomodulating polysaccharides. Appl Microb Biotec 60:258–274CrossRefGoogle Scholar
  2. 2.
    Zervakis G, Dimou D, Polemis Ε (2004) Fungal diversity and conservation in the Mediterranean area: recent advances in the inventory of Greek macromycetes. Mycol Balc 1:31–34Google Scholar
  3. 3.
    Barros L, Baptista P, Correia DM, Morais JS, Ferreira ICFR. (2007) Effects of conservation treatment and cooking on the chemical composition and antioxidant activity of Portuguese wild edible mushrooms. J Agric Food Chem 55:4781–4788CrossRefGoogle Scholar
  4. 4.
    Cheung PCK (2008) Nutritional value and health benefits of mushrooms. In: Mushrooms as functional foods, John Wiley and Sons, Inc., Hoboken, pp 71–109CrossRefGoogle Scholar
  5. 5.
    Mattila P, Konko K, Eurola M, Pihlava J-M, Astola J, Vahteristo L, Hietaniemi V, Kumpulainen J, Valtonen M, Piironen V (2001) Contents of vitamins, mineral elements, and some phenolic compounds in cultivated mushrooms. J Agric Food Chem 49:2343–2348CrossRefGoogle Scholar
  6. 6.
    Cheung LM, Cheung PCK, Ooi VEC (2003) Antioxidant activity and total phenolic of edible mushroom extracts. Food Chem 81:249–255CrossRefGoogle Scholar
  7. 7.
    Mau J, Huang P, Huang S, Chen C (2004) Antioxidant properties of methanolic extracts from Grifola frondosa, Morchella esculenta and Termitomyces albuminosus mycelia. Food Chem 87:111–118CrossRefGoogle Scholar
  8. 8.
    Barros L, Falcao S, Baptista P, Freire C, Vilas-Boas M, Ferreira ICFR. (2008) Antioxidant activity of Agaricus sp. mushrooms by chemical, biochemical and electrochemical assays. Food Chem 111:61–66CrossRefGoogle Scholar
  9. 9.
    Mizuno T (1999) The extraction and development of antitumor active polysaccharides from medicinal mushrooms in Japan. Int J Med Mushrooms 1:9–29CrossRefGoogle Scholar
  10. 10.
    Reshetnikov SV, Wasser SP, Tan KK (2001) Higher Basidiomycota as a source of antitumor and immunostimulating polysaccharides. Int J Med Mushrooms 3:361–394Google Scholar
  11. 11.
    King F, King T, Godson D, Manning L (1956) The chemistry of extractives from hardwoods. Part XXVIII. The occurrence of 3:4:3ʹ:5ʹ-tetrahydroxy- and 3:4:5:3΄:5-pentahydroxy-stilbene in Vouacapoua species. J Chem Soc 4477–4480Google Scholar
  12. 12.
    Ferrigni NR, Mclaughlin JL, Powell RG, Smith CR (1984) Isolation of piceatannol as the antileukaemic principle from the seeds of Euphorbia lagascae. J Nat Prod 47:347–352CrossRefGoogle Scholar
  13. 13.
    Bavaresco L, Fregoni M, Trevisan M, Mattivi F, Vrhovsek U, Flachetti R (2002) The occurrence of the stilbene piceatannol in grapes. Vitis 41:133–136Google Scholar
  14. 14.
    Rimando AM, Kalt W, Magee JB, Dewey J, Ballington J (2004) Resveratrol, pterostilbene and piceatannol in Vaccinium berries. J Agr Food Chem 52:4713–4719CrossRefGoogle Scholar
  15. 15.
    Ku KL, Chang PS, Cheng YC, Lien CY (2005) Production of stilbenoids from the callus of Arachis hypogaea. A novel source of the anticancer compound piceatannol. J Agr Food Chem 53:3877–3881CrossRefGoogle Scholar
  16. 16.
    Piotrowska H, Kucinska M, Murias M (2012) Biological activity of piceatannol: leaving the shadow of resveratrol. Mutat Res 750:60–82CrossRefGoogle Scholar
  17. 17.
    Kuo PL, Hus YL (2008) The grape and wine constituent piceatannol inhibits proliferation of human bladder cancer cells via blocking cell cycle progression and inducing Fas/membrane bound Fas ligand-mediated apoptotic pathway. Mol Nutr Food Res 52:408–418CrossRefGoogle Scholar
  18. 18.
    Ko YJ, Kim HH, Kim EJ, Katakura Y, Lee WS, Ryu CH (2013) Piceatannol inhibits mast cell-mediated allergic inflammation. Int J Mol Med 31:951–958CrossRefGoogle Scholar
  19. 19.
    Kahkonen MP, Hopia AI, Vuorela HJ, Rauha J, Pihlaja K, Kujala TS, Heinonen M (1999) Antioxidant activity of plant extracts containing phenolic compounds. J Agric Food Chem 47:3954–3962CrossRefGoogle Scholar
  20. 20.
    Mossman T (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 65:55–63CrossRefGoogle Scholar
  21. 21.
    Engeland M, Ramaekers FCS, Schutte B, Reutelingsperger CP (1996) A novel assay to measure loss of plasma membrane asymmetry during apoptosis of adherent cells in culture. Cytometry 24:131–139CrossRefGoogle Scholar
  22. 22.
    Sanchez-Moreno C, Larrauri JA, Saura-Calixto F (1998) A procedure to measure the antiradical efficiency of polyphenols. J Sci Food Agric 76:270–276CrossRefGoogle Scholar
  23. 23.
    Slinkard K, Singleton VL (1977) Total phenol analysis, automation and comparison with manual methods. Am J Enol Vitic 28:49–55Google Scholar
  24. 24.
    Zhishen J, Mengcheng T, Jianming W (1999) The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem 64:555–559CrossRefGoogle Scholar
  25. 25.
    Meyers KJ, Watkins CB, Pritts MP, Liu RH (2003) Antioxidant and antiproliferative activities of strawberries. J Agric Food Chem 51:6887–6892CrossRefGoogle Scholar
  26. 26.
    Yfanti P, Batistatou A, Manos G, Lekka ME (2015) The aromatic plant Satureja horvatii ssp. macrophylla Induces apoptosis and cell death to the A549 cancer cell line. Am J Plant Sci 6:2092–2103CrossRefGoogle Scholar
  27. 27.
    Kim MY, Seguin P, Ahn JK, Kim JJ, Chun SC, Kim EH, Seo SH, Kang EY, Kim SL, Park YJ, Ro HM, Chung IM (2008) Phenolic compound concentration and antioxidant activities of edible and medicinal mushrooms from Korea. J Agric Food Chem 56:7265–7270CrossRefGoogle Scholar
  28. 28.
    Keckes S, Gasic U, Cirkovic-Velickovic T, Milojkovic-Opsenica D, Natic M, Tesic Z (2013) The determination of phenolic profiles of Serbian unifloral honeys using ultra-high-performance liquid chromatography/high resolution accurate mass spectrometry. Food Chem 138:32–40CrossRefGoogle Scholar
  29. 29.
    Barros L, Ferreira M-J, Queiros B, Ferreira ICFR., Baptista P (2007) Total phenols, ascorbic acid, b-carotene and lycopene in Portuguese wild edible mushrooms and their antioxidant activities. Food Chem 103:413–419CrossRefGoogle Scholar
  30. 30.
    Barros L, Duenas M, Ferreira IC, Baptista P, Santos-Buelga C (2009) Phenolic acids determination by HPLC-DAD-ESI⁄MS in sixteen different Portuguese wild mushrooms species. Food Chem Toxicol 47:1076–1079CrossRefGoogle Scholar
  31. 31.
    Vaz J, Barros L, Martins A, Santos-Buelga C, Vasconcelos H, Ferreira I (2011) Chemical composition of wild edible mushrooms and antioxidant properties of their water soluble polysaccharidic and ethanolic fractions. Food Chem 126:610–616CrossRefGoogle Scholar
  32. 32.
    Elmastas M, Isildak O, Turkekul I, Temur N (2007) Determination of antioxidant activity and antioxidant compounds in wild edible mushrooms. J Food Comp Anal 20:337–345CrossRefGoogle Scholar
  33. 33.
    Tsai S-Y, Tsai H-L, Mau J-L (2007) Antioxidant properties of Agaricus blazei, Agrocybe cylindracea and Boletus edulis. LWT 40:1392–1402CrossRefGoogle Scholar
  34. 34.
    Vidovic S, Mujic I, Zekovic Z, Lepojevic Z, Tumbas V, Mujic A (2010) Antioxidant properties of selected Boletus mushrooms. Food Biophys 5:49–58CrossRefGoogle Scholar
  35. 35.
    Heleno SA, Barros L, Sousa MJ, Martins A, Ferreira ICFR. (2010) Tocopherols composition of Portuguese wild mushrooms with antioxidant capacity. Food Chem 119:1443–1450CrossRefGoogle Scholar
  36. 36.
    Puttaraju NG, Venkateshaiah SU, Dharmesh SM, Urs SMN, Somasundaram R (2006) Antioxidant activity of indigenous edible mushrooms. J Agric Food Chem 54:9764–9772CrossRefGoogle Scholar
  37. 37.
    Gursoy N, Sarikurkcu C, Cengiz M, Solak MH (2016) Antioxidant activities, metal contents, total phenolics and flavonoids of seven Morchella species. Food Chem Toxicol 47:2381–2388CrossRefGoogle Scholar
  38. 38.
    Tahidul I, Xiaoming Y, Baojun X (2016) Phenolic profiles, antioxidant capacities and metal chelating ability of edible mushrooms commonly consumed in China. LWT 72:423–431CrossRefGoogle Scholar
  39. 39.
    Woldegiorgis AZ, Abate D, Haki GD, Ziegler GR (2014) Antioxidant property of edible mushrooms collected from Ethiopia. Food Chem 157:30–36CrossRefGoogle Scholar
  40. 40.
    Palacios Ι, Lozano Μ, Moro C, D’Arrigo M, Rostagno M, Martνnez J, Garcνa-Lafuente A, Guillamon E, Villares A (2011) Antioxidant properties of phenolic compounds occurring in edible mushrooms. Food Chem 128:674–678CrossRefGoogle Scholar
  41. 41.
    Josiana A (2010) Wild mushrooms Clitocybe alexandri and Lepista inversa: In vitro antioxidant activity and growth inhibitor of human tumour cell line. Food Chem Toxicol 48:2881–2884CrossRefGoogle Scholar
  42. 42.
    Ren D, Jiao Y, Yang X, Yuan L, Guo J, Zhao Y (2015) Antioxidant and antitumor effects of polysaccharides from the fungus Pleurotus abalonus. Chem Biol Interact 237:166–674CrossRefGoogle Scholar
  43. 43.
    Larrosa M, Tomas-Barberan FA, Espin JC (2004) The grape and wine polyphenol piceatannol is a potent inducer of apoptosis in human SK-Mel-28 melanoma cells. Eur J Nutr 43:275–284CrossRefGoogle Scholar
  44. 44.
    Rossi M, Caruso F, Opazo C, Salciccioli J (2008) Crystal and molecular structure of piceatannol; scavenging features of resveratrol and piceatannol on hydroxyl and peroxyl radicals and docking with transthyretin. J Agric Food Chem 56:10557–10566CrossRefGoogle Scholar
  45. 45.
    Kim Y, Park C, Lee J, Kim G, Lee W, Choi Y, Ryu C (2008) Induction of apoptosis by piceatannol in human leukemic U937 cells through down-regulation of Bcl-2 and activation of caspases. Oncol Rep 19:961–967Google Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2017

Authors and Affiliations

  1. 1.Department of Chemistry, Laboratory of BiochemistryUniversity of IoanninaIoanninaGreece
  2. 2.Department of Crop Science, Laboratory of Vegetable CropsAgricultural University of AthensAthensGreece

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