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European Food Research and Technology

, Volume 244, Issue 5, pp 881–891 | Cite as

Antimicrobial activities of bamboo (Phyllostachys heterocycla cv. Pubescens) leaf essential oil and its major components

  • Cui Tao
  • Jian Wu
  • Ya Liu
  • Mei Liu
  • Ruipu Yang
  • Zhaolin Lv
Original Paper
  • 223 Downloads

Abstract

This research aimed to investigate the seasonal variations and antibacterial activities of essential oils (EOs) and their major components from bamboo leaves. The Phyllostachys heterocycla cv. Pubescens leaves have undergone steam distillation to prepare EOs; leaves collected in autumn had the highest amount of EOs (0.33%). Chemical characterization by gas chromatography/mass spectrometry (GC/MS) allowed the identification of 36 compounds. The major compounds (%) were ketones (52.25), alcohols (17.79), carboxylic acids (9.41) and alkane hydrocarbons (7.68), which included 6, 5, 7 and 9 distinct compounds, respectively. Antibacterial activities were registered against Gram-negative bacteria [Escherichia coli (minimum inhibitory concentration [MIC] = 2.25 mg/mL)], Gram-positive bacteria [Bacillus subtilis (MIC = 2.25 mg/mL)] and Saccharomyces cerevisiae. The antibacterial effect of the three major monomeric compounds (tricosane, cedrol and hexadecanoic acid) in bamboo leaf EOs and their mixtures was studied. We showed for the first time that tricosane had antimicrobial activity. We found that cedrol had the strongest antibacterial effect, followed by hexadecanoic acid. Cedrol at 5 mg/mL had the largest inhibition zones of up to 14.93 mm against Flavobacterium SHL45. The antimicrobial effect of the equal mixture ratio of the three monomers was higher than that when tricosane and hexadecanoic acid were tested alone, and was also higher than that of the combination of any two of them.

Keywords

Antibacterial activity Phyllostachys heterocycla cv. Pubescens leaves Essential oils Chemical components 

Notes

Acknowledgements

The authors wish to acknowledge the support from the Science and technology research project of Beijing Forestry University (2015-01) and “National Science-technology Support Plan Projects, research and development of botanical novel green preservative” (Project number: 2015BAD16B01).

Author contributions

J. Wu analyzed data, drafted the manuscript and revised it critically for important intellectual content. ZL. Lv designed the study and supervised the research group. Y. Liu collected test data. M. Liu carried out experiments. C. Tao interpreted the results.

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest. The founding sponsors had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, and in the decision to publish the results.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2017

Authors and Affiliations

  • Cui Tao
    • 1
  • Jian Wu
    • 1
  • Ya Liu
    • 1
  • Mei Liu
    • 1
  • Ruipu Yang
    • 1
  • Zhaolin Lv
    • 2
  1. 1.College of Biological Sciences and BiotechnologyBeijing Forestry UniversityBeijingChina
  2. 2.Testing & Analysis CenterBeijing Forestry UniversityBeijingChina

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