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Purification, characterization, and action mechanism of plantaricin DL3, a novel bacteriocin against Pseudomonas aeruginosa produced by Lactobacillus plantarum DL3 from Chinese Suan-Tsai

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Abstract

A novel bacteriocin produced by Lactobacillus plantarum DL3 isolated from Suan-Tsai, a traditional Chinese fermented cabbage, was designated as plantaricin DL3. It was purified by ethyl acetate extraction followed by gel filtration and high-performance liquid chromatography. The molecular weight of plantaricin DL3 was determined as 2149 Da by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF–MS) analysis. The amino acid sequence of plantaricin DL3 was predicted to be VGPGAINAGTYLVSRELFER by MALDI-TOF–MS/MS. This bacteriocin exhibited broad-spectrum antibacterial activity against Gram-positive and Gram-negative bacteria, especially Pseudomonas aeruginosa, high thermal stability (15 min, 121 °C) and narrow pH stability (pH 2.5–5.5). The mechanism of action of this bacteriocin was responsible for the disruption of cell wall, accompanied with the leakage of proteins. These results suggested that plantaricin DL3 has potential applications in the control of Pseudomonas spp. in aquatic products.

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References

  1. Gram L, Dalgaard P (2002) Fish spoilage bacteria—problems and solutions. Curr Opin Biotechnol 13(3):262–266

    Article  CAS  Google Scholar 

  2. Liu H, Zhang L, Yi H, Han X, Chi C (2016) Identification and characterization of plantaricin Q7, a novel plantaricin produced by Lactobacillus plantarum Q7. LWT Food Sci Technol 71:386–390

    Article  CAS  Google Scholar 

  3. Al-Haddad KSH, Al-Qassemi RAS, Robinson RK (2005) The use of gaseous ozone and gas packaging to control populations of Salmonella infantis and Pseudomonas aeruginosa on the skin of chicken portions. Food Control 16(5):405–410

    Article  CAS  Google Scholar 

  4. Lu H, Shao X, Cao J, Ou C, Pan D (2016) Antimicrobial activity of eucalyptus essential oil against Pseudomonas in vitro and potential application in refrigerated storage of pork meat. Int J Food Sci Technol 51(4):994–1001

    Article  CAS  Google Scholar 

  5. Ghanbari M, Jami M, Domig KJ, Kneifel W (2013) Seafood biopreservation by lactic acid bacteria—a review. LWT Food Sci Technol 54(2):315–324

    Article  CAS  Google Scholar 

  6. Wan Norhana MN, Poole SE, Deeth HC, Dykes GA (2010) The effects of temperature, chlorine and acids on the survival of Listeria and Salmonella strains associated with uncooked shrimp carapace and cooked shrimp flesh. Food Microbiol 27(2):250–256

    Article  CAS  Google Scholar 

  7. Matamoros S, Pilet MF, Gigout F, Prévost H, Leroi F (2009) Selection and evaluation of seafood-borne psychrotrophic lactic acid bacteria as inhibitors of pathogenic and spoilage bacteria. Food Microbiol 26(6):638–644

    Article  CAS  Google Scholar 

  8. Todorov SD, Holzapfel W, Nero LA (2015) Characterization of a novel bacteriocin produced by Lactobacillus plantarum ST8SH and some aspects of its mode of action. Ann Microbiol 66(3):949–962

    Article  Google Scholar 

  9. Zhao R, Duan G, Yang T, Niu S, Wang Y (2015) Purification, characterization and antibacterial mechanism of bacteriocin from Lactobacillus Acidophilus XH1. Trop J Pharm Res 14(6):989

    Article  CAS  Google Scholar 

  10. Liu G, Song Z, Yang X, Gao Y, Wang C, Sun B (2016) Antibacterial mechanism of bifidocin A, a novel broad-spectrum bacteriocin produced by Bifidobacterium animalis BB04. Food Control 62:309–316

    Article  CAS  Google Scholar 

  11. Bali V, Panesar PS, Bera MB, Kennedy JF (2014) Bacteriocins: recent trends and potential applications. Crit Rev Food Sci 56(5):817–834

    Article  Google Scholar 

  12. Zhao S, Han J, Bie X, Lu Z, Zhang C, Lv F (2016) Purification and characterization of plantaricin JLA-9: a novel bacteriocin against Bacillus spp. produced by Lactobacillus plantarum JLA-9 from Suan-Tsai, a traditional Chinese fermented cabbage. J Agric Food Chem 64(13):2754–2764

    Article  CAS  Google Scholar 

  13. Hu M, Zhao H, Zhang C, Yu J, Lu Z (2013) Purification and characterization of plantaricin 163, a novel bacteriocinp produced by Lactobacillus plantarum 163 isolated from traditional Chinese fermented vegetables. J Agric Food Chem 61(47):11676–11682

    Article  CAS  Google Scholar 

  14. Fang Z, Zhao HF, Bai FL, Dziugan P, Liu Y, Zhang BL (2014) Purification and characterisation of the bacteriocin produced by Lactobacillus plantarum, isolated from Chinese pickle. Czech J Food Sci 32(5):430–436

    Google Scholar 

  15. Song DF, Zhu MY, Gu Q (2014) Purification and characterization of Plantaricin ZJ5, a new bacteriocin produced by Lactobacillus plantarum ZJ5. PLoS One 9(8):e105549

    Article  Google Scholar 

  16. Ström K, Sjögren J, Broberg A, Schnürer J (2002) Lactobacillus plantarum MiLAB 393 produces the antifungal cyclic dipeptides cyclo(L-Phe-L-Pro) and cyclo(L-Phe-trans-4-OH-L-Pro) and 3-phenyllactic acid. Appl Environ Microb 68(9):4322–4327

    Article  Google Scholar 

  17. Chahad OB, El Bour M, Calo-Mata P, Boudabous A, Barros-Velazquez J (2012) Discovery of novel biopreservation agents with inhibitory effects on growth of food-borne pathogens and their application to seafood products. Res Microbiol 163(1):44–54

    Article  CAS  Google Scholar 

  18. Liu G, Ren L, Song Z, Wang C, Sun B (2015) Purification and characteristics of bifidocin A, a novel bacteriocin produced by Bifidobacterium animals BB04 from centenarians’ intestine. Food Control 50:889–895

    Article  CAS  Google Scholar 

  19. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72(1–2):248–254

    Article  CAS  Google Scholar 

  20. Schägger H, Jagow GV (1987) Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal Biochem 166(2):368

    Article  Google Scholar 

  21. Kumarathasan P, Mohottalage S, Goegan P, Vincent R (2005) An optimized protein in-gel digest method for reliable proteome characterization by MALDI-TOF-MS analysis. Anal Biochem 346(1):85–89

    Article  CAS  Google Scholar 

  22. Zhu X, Zhao Y, Sun Y, Gu Q (2014) Purification and characterisation of plantaricin ZJ008, a novel bacteriocin against Staphylococcus spp. from Lactobacillus plantarum ZJ008. Food Chem 165:216–223

    Article  CAS  Google Scholar 

  23. Wen LS, Philip K, Ajam N (2016) Purification, characterization and mode of action of plantaricin K25 produced by Lactobacillus plantarum. Food Control 60:430–439

    Article  CAS  Google Scholar 

  24. Chen YS, Wang YC, Chow YS, Yanagida F, Liao CC, Chiu CM (2014) Purification and characterization of plantaricin Y, a novel bacteriocin produced by Lactobacillus plantarum 510. Arch Microbiol 196(3):193

    Article  CAS  Google Scholar 

  25. Atrih A, Rekhif N, Moir AJG, Lebrihi A, Lefebvre G (2001) Mode of action, purification and amino acid sequence of plantaricin C19, an anti-Listeria bacteriocin produced by Lactobacillus plantarum C19. Int J Food Microbiol 68(1–2):93–104

    Article  CAS  Google Scholar 

  26. Todorov SD, Dicks LMT (2005) Effect of growth medium on bacteriocin production by Lactobacillus plantarum ST194BZ, a strain isolated from Boza. Food Technol Biotech 43(2):165–173

    CAS  Google Scholar 

  27. Sommerer N, Centeno D, Rossignol M (2007) Peptide mass fingerprinting: identification of proteins by MALDI-TOF. Methods Mol Biol 355(355):219–234

    CAS  Google Scholar 

  28. Gong HS, Meng XC, Wang H (2010) Plantaricin MG active against Gram-negative bacteria produced by Lactobacillus plantarum KLDS1.0391 isolated from “Jiaoke”, a traditional fermented cream from China. Food Control 21(1):89–96

    Article  CAS  Google Scholar 

  29. Powell JE, Witthuhn RC, Todorov SD, Lmt D (2007) Characterization of bacteriocin ST8KF produced by a kefir isolate Lactobacillus plantarum ST8KF. Int Dairy J 17(3):190–198

    Article  CAS  Google Scholar 

  30. Holo H, Jeknic Z, Daeschel M, Stevanovic S, Nes IF (2001) Plantaricin W from Lactobacillus plantarum belongs to a new family of two-peptide lantibiotics. Microbiol 147(3):643–651

    Article  CAS  Google Scholar 

  31. Castro MP, Palavecino NZ, Herman C, Garro OA, Campos CA (2011) Lactic acid bacteria isolated from artisanal dry sausages: characterization of antibacterial compounds and study of the factors affecting bacteriocin production. Meat Sci 87(4):321–329

    Article  CAS  Google Scholar 

  32. Magnusson J, Schnurer J (2001) Lactobacillus coryniformis subsp. coryniformis strain Si3 produces a broad-spectrum proteinaceous antifungal compound. Appl Environ Microbiol 67(1):1–5

    Article  CAS  Google Scholar 

  33. Campos CA, Rodríguez Ó, Calo-Mata P, Prado M, Barros-Velázquez J (2006) Preliminary characterization of bacteriocins from Lactococcus lactis, Enterococcus faecium and Enterococcus mundtii strains isolated from turbot (Psetta maxima). Food Res Int 39(3):356–364

    Article  CAS  Google Scholar 

  34. Bajpai VK, Sharma A, Baek K-H (2013) Antibacterial mode of action of Cudrania tricuspidata fruit essential oil, affecting membrane permeability and surface characteristics of food-borne pathogens. Food Control 32(2):582–590

    Article  CAS  Google Scholar 

  35. Yi L, Dang J, Zhang L, Wu Y, Liu B, Lü X (2016) Purification, characterization and bactericidal mechanism of a broad spectrum bacteriocin with antimicrobial activity against multidrug-resistant strains produced by Lactobacillus coryniformis XN8. Food Control 67:53–62

    Article  CAS  Google Scholar 

  36. Yoneyama F, Imura Y, Ohno K, Zendo T, Nakayama J, Matsuzaki K, Sonomoto K (2009) Peptide-Lipid Huge Toroidal Pore, a new antimicrobial mechanism mediated by a Lactococcal Bacteriocin, Lacticin Q. Antimicrob Agents Chemother 53(8):3211–3217

    Article  CAS  Google Scholar 

  37. Li Y-Q, Han Q, Feng J-L, Tian W-L, Mo H-Z (2014) Antibacterial characteristics and mechanisms of ɛ-poly-lysine against Escherichia coli and Staphylococcus aureus. Food Control 43:22–27

    Article  CAS  Google Scholar 

  38. Zhao L, Zhang H, Hao T, Li S (2015) In vitro antibacterial activities and mechanism of sugar fatty acid esters against five food-related bacteria. Food Chem 187:370–377

    Article  CAS  Google Scholar 

  39. Sitohy MZ, Mahgoub SA, Osman AO (2012) In vitro and in situ antimicrobial action and mechanism of glycinin and its basic subunit. Int J Food Microbiol 154(1–2):19–29

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This study was supported by the Fundamental Research Funds for the Central Universities (No. 2015ZCQ-SW-05) and the Research Project from Science & Technology Department of Liaoning Province of China (No. 2015103020).

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Author notes

  1. Bolin Zhang and Fengling Bai contributed equally to this work and should be considered co-correspondence authors.

Authors and Affiliations

  1. College of Biological Science and Biotechnology, Beijing Forestry University, No.35 Tsinghua East Road Haidian District, Beijing, 100083, People’s Republic of China

    Xinran Lv, Yu Jie, Bolin Zhang & Hongfei Zhao

  2. College of Food Science and Engineering, Bohai University, 19 Keji Road, Songshan New District, Jinzhou City, 121013, Liaoning Province, China

    Xinran Lv, Yang Lin, Mengtong Sun, Fengling Bai & Jianrong Li

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  1. Xinran Lv
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  2. Yang Lin
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  3. Yu Jie
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  5. Bolin Zhang
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Correspondence to Bolin Zhang or Fengling Bai.

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Lv, X., Lin, Y., Jie, Y. et al. Purification, characterization, and action mechanism of plantaricin DL3, a novel bacteriocin against Pseudomonas aeruginosa produced by Lactobacillus plantarum DL3 from Chinese Suan-Tsai. Eur Food Res Technol 244, 323–331 (2018). https://doi.org/10.1007/s00217-017-2958-3

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  • DOI: https://doi.org/10.1007/s00217-017-2958-3

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