Abstract
Response surface methodology was used to optimize tea polyphenols-loaded chitosan nanoclusters preparation conditions, including carboxymethyl chitosan concentration, chitosan hydrochloride concentration and amount of tea polyphenols. The responses particle size and entrapment efficiency of nanoclusters were studied. The optimum conditions of carboxymethyl chitosan concentration, chitosan hydrochloride concentration and amount of tea polyphenols were found to be 3.63, 1.19 and 10.94 mg/mL, respectively. The optimized particle size was 301 nm, and entrapment efficacy of nanoclusters was added up to 83.7%. The results demonstrated that Box–Behnken design methodology was an effective way to obtain the optimal formulation of tea polyphenols-loaded chitosan nanoclusters, and the nanoclusters complexation synthesizing through ionic gelation between carboxymethyl chitosan and chitosan hydrochloride was good biomaterials, which could be successfully used to encapsulate tea polyphenols.
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References
Pan X, Niu G, Liu H (2003) Chem Eng Process 42:129–133
Yang CS, Lambert JD, Sang S (2009) Arch Toxicol 83:11–21
Khan N, Mukhtar H (2008) Cancer Lett 269:269–280
Higdon JV, Frei B (2003) Crit Rev Food Sci Nutr 43:89–143
Chen YA, Hsu KY (2009) Arch Pharm Res 32:149–154
Nakagawa K, Ninomiya M, Okubo T, Aoi N, Juneja LR, Kim M, Yamanaka K, Miyazawa T (1999) J Agric Food Chem 47:3967–3973
Henning SM, Niu Y, Liu Y, Lee NH, Hara Y, Thames GD, Minutti RR, Carpenter CL, Wang H, Heber D (2005) J Nutr Biochem 16:610–616
Woitiski CB, Veiga F, Ribeiro A, Neufeld R (2009) Eur J Pharm Biopharm 73:25–33
Barichello JM, Morishita M, Takayama K, Nagai T (1999) Drug Dev Ind Pharm 25:471–476
Hu B, Pan C, Sun Y, Hou Z, Ye H, Zeng X (2008) J Agric Food Chem 56:7451–7458
Qi L, Xu Z, Chen M (2007) Eur J Cancer 43:184–193
Bowman K, Leong KW (2006) Int J Nanomedicine 1:117–128
Chellat F, Tabrizian M, Dumitriu S, Chornet E, Magny P, Rivard CH, Yahia L (2000) J Biomed Mater Res 51:107–116
Zharov VP, Kim JW, Curiel DT, Everts M (2005) Nanomed-nanotechnol 1:326–345
El-Shabouri MH (2002) Int J Pharm 249:101–108
Chen F, Zhang ZR, Huang Y (2007) Int J Pharm 336:166–173
Sayın B, Somavarapu S, Li XW, Thanou M, Sesardic D, Alpar HO, Senel S (2008) Int J Pharm 363:139–148
Qi L, Xu Z (2006) Bioorg Med Chem Lett 16:4243–4245
Borchard G (2001) Adv Drug Deliver Rev 52:145–150
Mansouri S, Lavigne P, Corsi K, Benderdour M, Beaumont E, Fernandes JC (2004) Eur J Pharm Biopharm 57:1–8
Zhang L, Kosaraju SL (2007) Eur Polym J 43:2956–2966
Li F, Wang F, Yu F, Fang Y, Xin Z, Yang F, Xu J, Zhao L, Hu Q (2008) Food Chem 111:165–170
Gao Y, Liu X, Qiao X (2006) Hei Long Jiang Medicine (China) 19: 108–110
Huang J, Huang K, Liu S, Luo Q, Xu M (2007) J Colloid Interface Sci 315:407–414
Sarmento B, Ribeiro AJ, Veiga F, Ferreira DC, Neufeld RJ (2007) J Nanosci Nanotechnol 7:2833–2841
Gazori T, Khoshayand MR, Azizi E, Yazdizade P, Nomani A, Haririan I (2009) Carbohydr Polym 77:599–606
de Moura MR, Aouada FA, Mattoso LHC (2008) J Colloid Interface Sci 321:477–483
Ajun W, Yan S, Li G, Huili L (2009) Carbohydr Polym 75:566–574
Gan Q, Wang T (2007) Colloid Surface B 59:24–34
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This work was supported by the National Natural Science Foundation of China (30871743), the 111 Project of Education Ministry of China (B07030) and the National High Technology Research and Development Program of China (2007AA100403).
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Liang, J., Li, F., Fang, Y. et al. Response surface methodology in the optimization of tea polyphenols-loaded chitosan nanoclusters formulations. Eur Food Res Technol 231, 917–924 (2010). https://doi.org/10.1007/s00217-010-1341-4
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DOI: https://doi.org/10.1007/s00217-010-1341-4